COMBINED AUTORADIOGRAPHY AND ELECTRON MICROSCOPY OF THIN SECTIONS OF INTESTINAL EPITHELIAL CELLS OF THE MOUSE LABELED WITH H3-THYMIDINE

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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Immunocytochemical localization of ornithine transcarbamylase in rat intestinal mucosa. Light and electron microscopic study.

Journal of Histochemistry & Cytochemistry ◽

10.1177/36.1.3275711 ◽

1988 ◽

Vol 36 (1) ◽

pp. 29-35 ◽

Cited By ~ 11

Author(s):

Y Hamano ◽

H Kodama ◽

M Yanagisawa ◽

Y Haraguchi ◽

M Mori ◽

...

Keyword(s):

Electron Microscopy ◽

Small Intestine ◽

Epithelial Cells ◽

Light Microscopy ◽

Intestinal Mucosa ◽

Intestinal Epithelial Cells ◽

Protein A ◽

Intestinal Epithelial ◽

Electron Microscopic ◽

Small Intestinal

We investigated light and electron microscopic localization of ornithine transcarbamylase (OTC) in rat intestinal mucosa. In the immunoblotting assay of OTC-related protein, a single protein band with a molecular weight of about 36,500 is observed in extracts of liver and small intestinal mucosa but is not observed in those of stomach and large intestine. For light microscopy, tissue slices of the digestive system were embedded in Epon and stained by using anti-bovine OTC rabbit IgG and the immunoenzyme technique. For electron microscopy, slices of these and the liver tissues were embedded in Lowicryl K4M and stained by the protein A-gold technique. By light microscopy, the absorptive epithelial cells of duodenum, jejunum, and ileum stained positively for OTC, but stomach, large intestine, rectum, and propria mucosa of small intestine were not stained. Electron microscopy showed that gold particles representing the antigenic sites for OTC were confined to the mitochondrial matrix of hepatocytes and small intestinal epithelial cells. However, the enzyme was detected in mitochondria of neither liver endothelial cells, submucosal cells of small intestine, nor large intestinal epithelial cells. Labeling density of mitochondria in the absorptive epithelial cells of duodenum, jejunum, and ileum was about half of that in liver cells.

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Intestinal cyto differentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme

Development ◽

10.1242/dev.82.1.163 ◽

1984 ◽

Vol 82 (1) ◽

pp. 163-176

Author(s):

Atsuko Ishizuya-Oka ◽

Takeo Mizuno

Keyword(s):

Electron Microscopy ◽

Epithelial Cells ◽

Intestinal Epithelium ◽

Intestinal Epithelial Cells ◽

Goblet Cells ◽

Secretory Cells ◽

Chick Embryos ◽

Intestinal Epithelial ◽

Self Differentiation

The inductive action of duodenal mesenchyme on the cytodifferentiation of stomach endoderm in chick embryos was investigated in vitro with electron microscopy and immunofluorescence. Morphologically undifferentiated endoderm of the stomach of a 4-day embryo could differentiate only into a mucous secretory epithelium when cultured in the absence of mesenchyme. However, when cultivated in recombination with 6-day duodenal mesenchyme, most cells of 4-day stomach endoderm differentiated into intestinal absorptive cells possessing striated border and sucrase, and goblet cells, but not into stomach-type mucous secretory cells. In contrast, when 4-day stomach endoderm was cultured recombined with mesenchyme of embryonic digestive organs other than intestine, none of the stomach endoderm cells differentiated into intestinal epithelial cells. The competence of stomach endoderm for intestinal cytodifferentiation decreased rapidly with development, but remained until relatively later stages in the gizzard region. The present investigation demonstrates that duodenal mesenchyme can induce stomach endoderm, which has acquired the potency for self-differentiation into stomach-type epithelium, to cytodifferentiate into intestinal epithelium.

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Transmission Electron Microscopy of Microvilli of Intestinal Epithelial Cells in Celiac Disease in Remission and Transient Gluten Enteropathy in Children After a Gluten-Free Diet

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/00005176-199304000-00008 ◽

1993 ◽

Vol 16 (3) ◽

pp. 269-272 ◽

Cited By ~ 5

Author(s):

Antoni Dyduch ◽

Krystyna Karczewska ◽

Henryk Grzybek ◽

Marcin Kamiński

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Celiac Disease ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Gluten Free Diet ◽

Intestinal Epithelial ◽

Gluten Free ◽

Transmission Electron ◽

Gluten Enteropathy

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Membrane-bound vesicles associated with the microvilli in the midgut of the freshwater microcrustacean, Daphnia magna

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076135 ◽

1983 ◽

Vol 41 ◽

pp. 480-481

Author(s):

Patricia L. Jansma

Keyword(s):

Daphnia Magna ◽

Intestinal Epithelial Cells ◽

Uranyl Acetate ◽

Intestinal Epithelial ◽

Chlamydomonas Reinhardii ◽

Thin Sections ◽

Saturated Water ◽

Cacodylate Buffer ◽

Membrane Bound ◽

Ethanol Series

The presence of the membrane bound vesicles or blebs on the intestinal epithelial cells has been demonstrated in a variety of vertebrates such as chicks, piglets, hamsters, and humans. The only invertebrates shown to have these microvillar blebs are two species of f1ies. While investigating the digestive processes of the freshwater microcrustacean, Daphnia magna, the presence of these microvillar blebs was noticed.Daphnia magna fed in a suspension of axenically grown green alga, Chlamydomonas reinhardii for one hour were narcotized with CO2 saturated water. The intestinal tracts were excised in 2% glutaraldehyde in 0.2 M cacodyl ate buffer and then placed in fresh 2% glutaraldehyde for one hour. After rinsing in 0.1 M cacodylate buffer, the sample was postfixed in 2% OsO4, dehydrated with a graded ethanol series, infiltrated and embedded with Epon-Araldite. Thin sections were stained with uranyl acetate and Reynolds lead citrate before viewing with the Philips EM 200.

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