Intestinal cyto differentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme

The inductive action of duodenal mesenchyme on the cytodifferentiation of stomach endoderm in chick embryos was investigated in vitro with electron microscopy and immunofluorescence. Morphologically undifferentiated endoderm of the stomach of a 4-day embryo could differentiate only into a mucous secretory epithelium when cultured in the absence of mesenchyme. However, when cultivated in recombination with 6-day duodenal mesenchyme, most cells of 4-day stomach endoderm differentiated into intestinal absorptive cells possessing striated border and sucrase, and goblet cells, but not into stomach-type mucous secretory cells. In contrast, when 4-day stomach endoderm was cultured recombined with mesenchyme of embryonic digestive organs other than intestine, none of the stomach endoderm cells differentiated into intestinal epithelial cells. The competence of stomach endoderm for intestinal cytodifferentiation decreased rapidly with development, but remained until relatively later stages in the gizzard region. The present investigation demonstrates that duodenal mesenchyme can induce stomach endoderm, which has acquired the potency for self-differentiation into stomach-type epithelium, to cytodifferentiate into intestinal epithelium.

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Expression of Human Decay-Accelerating Factor on Intestinal Epithelium of Transgenic Mice Does Not Facilitate Infection by the Enteral Route

Journal of Virology ◽

10.1128/jvi.03468-14 ◽

2015 ◽

Vol 89 (8) ◽

pp. 4311-4318 ◽

Cited By ~ 7

Author(s):

Jieyan Pan ◽

Lili Zhang ◽

Matthew A. Odenwald ◽

Le Shen ◽

Jerrold R. Turner ◽

...

Keyword(s):

Epithelial Cells ◽

Transgenic Mice ◽

Intestinal Epithelium ◽

Intestinal Epithelial Cells ◽

Apical Cell ◽

Oral Route ◽

Intestinal Epithelial ◽

Human Intestinal Epithelial Cells ◽

Cvb3 Infection

ABSTRACTIn vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal injection, infection by the enteral route is very inefficient. Murine DAF, unlike human DAF, does not bind virus, and we hypothesized that the absence of an accessible receptor on the intestinal surface is an important barrier to infection by the oral route. We generated transgenic mice that express human DAF specifically on intestinal epithelium and measured their susceptibility to infection by a DAF-binding CVB3 isolate. Human DAF permitted CVB3 to bind to the intestinal surfaceex vivoand to infect polarized monolayers of small-intestinal epithelial cells derived from DAF transgenic mice. However, expression of human DAF did not facilitate infection by the enteral route either in immunocompetent animals or in animals deficient in the interferon alpha/beta receptor. These results indicate that the absence of an apical receptor on intestinal epithelium is not the major barrier to infection of mice by the oral route.IMPORTANCECVB3 infection of human intestinal epithelial cells depends on DAF at the apical cell surface, and expression of human DAF on murine intestinal epithelial cells permits their infectionin vitro. However, expression of human DAF on the intestinal surface of transgenic mice did not facilitate infection by the oral route. Although the role of intestinal DAF in human infection has not been directly examined, these results suggest that DAF is not the critical factor in mice.

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A novel two-compartment barrier model for investigating nanoparticle transport in fish intestinal epithelial cells

Environmental Science Nano ◽

10.1039/c5en00226e ◽

2016 ◽

Vol 3 (2) ◽

pp. 388-395 ◽

Cited By ~ 11

Author(s):

Mark Geppert ◽

Laura Sigg ◽

Kristin Schirmer

Keyword(s):

Rainbow Trout ◽

Epithelial Cells ◽

Intestinal Epithelium ◽

Intestinal Epithelial Cells ◽

Intestinal Barrier ◽

Intestinal Epithelial ◽

Nanoparticle Transport ◽

Barrier Model

We introduce a novel in vitro rainbow trout intestinal barrier model and demonstrate its suitability for investigating nanoparticle transport across the intestinal epithelium.

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Aggregation Substance Increases Adherence and Internalization, but Not Translocation, of Enterococcus faecalisthrough Different Intestinal Epithelial Cells In Vitro

Infection and Immunity ◽

10.1128/iai.68.10.6044-6047.2000 ◽

2000 ◽

Vol 68 (10) ◽

pp. 6044-6047 ◽

Cited By ~ 49

Author(s):

S. Sartingen ◽

E. Rozdzinski ◽

A. Muscholl-Silberhorn ◽

R. Marre

Keyword(s):

Epithelial Cells ◽

Enterococcus Faecalis ◽

Intestinal Epithelium ◽

Intestinal Epithelial Cells ◽

Bacterial Adherence ◽

Intestinal Epithelial ◽

Aggregation Substance

ABSTRACT The aggregation substance of Enterococcus faecalisincreased bacterial adherence to and internalization by epithelial cells originating from the colon and duodenum but not by cells derived from the ileum. However, enterococcal translocation through monolayers of intestinal epithelium was not observed.

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Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123283 ◽

1992 ◽

Vol 50 (1) ◽

pp. 576-577

Author(s):

Julian P. Heath ◽

Buford L. Nichols ◽

László G. Kömüves

Keyword(s):

Electron Microscopy ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Three Dimensional ◽

Intestinal Epithelial ◽

Cell Surfaces ◽

Basal Surface ◽

Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

Nutrients ◽

10.3390/nu13020328 ◽

2021 ◽

Vol 13 (2) ◽

pp. 328 ◽

Cited By ~ 1

Author(s):

Claudio Salaris ◽

Melania Scarpa ◽

Marina Elli ◽

Alice Bertolini ◽

Simone Guglielmetti ◽

...

Keyword(s):

Immune Response ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Plaque Assay ◽

Immune Response Gene ◽

Intestinal Epithelial ◽

Antiviral Immune Response ◽

Qrt Pcr ◽

Anti Inflammatory

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.

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Berberine Ameliorates Pro-inflammatory Cytokine-Induced Endoplasmic Reticulum Stress in Human Intestinal Epithelial Cells In Vitro

Inflammation ◽

10.1007/s10753-011-9385-6 ◽

2011 ◽

Vol 35 (3) ◽

pp. 841-849 ◽

Cited By ~ 28

Author(s):

Xianhua Hao ◽

Anlong Yao ◽

Jianfeng Gong ◽

Weiming Zhu ◽

Ning Li ◽

...

Keyword(s):

Endoplasmic Reticulum ◽

Epithelial Cells ◽

Endoplasmic Reticulum Stress ◽

Inflammatory Cytokine ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial ◽

Human Intestinal Epithelial Cells

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The effect of berberine in vitro on tight junctions in human Caco-2 intestinal epithelial cells

Fitoterapia ◽

10.1016/j.fitote.2009.02.005 ◽

2009 ◽

Vol 80 (4) ◽

pp. 241-248 ◽

Cited By ~ 36

Author(s):

Lili Gu ◽

Ning Li ◽

Qiurong Li ◽

Qiang Zhang ◽

Chengyang Wang ◽

...

Keyword(s):

Epithelial Cells ◽

Tight Junctions ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial

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TFF3 and EGF Induce Different Migration Patterns of Intestinal Epithelial Cells In Vitro and Trigger Increased Internalization of E-cadherin

Cellular Physiology and Biochemistry ◽

10.1159/000107519 ◽

2007 ◽

Vol 20 (5) ◽

pp. 329-346 ◽

Cited By ~ 32

Author(s):

Uwe Dürer ◽

Roland Hartig ◽

Susanne Bang ◽

Lars Thim ◽

Werner Hoffmann

Keyword(s):

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial ◽

Migration Patterns ◽

E Cadherin

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A σ28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176

Infection and Immunity ◽

10.1128/iai.74.1.769-772.2006 ◽

2006 ◽

Vol 74 (1) ◽

pp. 769-772 ◽

Cited By ~ 35

Author(s):

Scarlett Goon ◽

Cheryl P. Ewing ◽

Maria Lorenzo ◽

Dawn Pattarini ◽

Gary Majam ◽

...

Keyword(s):

Epithelial Cells ◽

Campylobacter Jejuni ◽

Diarrheal Disease ◽

Intestinal Epithelial Cells ◽

Disease Model ◽

Intestinal Epithelial ◽

Model Expression

ABSTRACT A Campylobacter jejuni 81-176 mutant in Cj0977 was fully motile but reduced >3 logs compared to the parent in invasion of intestinal epithelial cells in vitro. The mutant was also attenuated in a ferret diarrheal disease model. Expression of Cj0977 protein was dependent on a minimal flagella structure.

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