scholarly journals Structure and mass analysis of 14S dynein obtained from Tetrahymena cilia.

1988 ◽  
Vol 106 (1) ◽  
pp. 127-132 ◽  
Author(s):  
S P Marchese-Ragona ◽  
J S Wall ◽  
K A Johnson
Keyword(s):  
Microscopic Analysis ◽  
Immunoblot Analysis ◽  
Electron Microscopic ◽  
Average Mass ◽  
Transmission Electron ◽  
Sds Page ◽  
Transmission Electron Microscopic ◽  
Scanning Transmission ◽  
Tail Structure ◽  
Transmission Electron Microscopic Analysis

Scanning transmission electron microscopic analysis revealed that the 14S fraction of Tetrahymena dynein was of a mixture of two types of particles in approximately equal proportions. The 14S dynein molecules were roughly ellipsoid in shape with approximate axes of 9.5 and 14.5 nm. About half of the particles had tails 20-24-nm long. By the integration of electron scattering intensities, particles with tails had an average mass of 510 kD with a SD of 90 kD. The globular heads of both types of particles had an average mass of 330 kD with a SD of 60 kD. The mass of the tail structure was about 180 kD. By SDS-PAGE, the 14S dynein consisted of two high molecular mass polypeptides above 300 kD that could be distinguished by immunoblot analysis.

The number of moults in the egg of sealworm, Pseudoterranova decipiens (Nematoda: Ascaridoidea): an ultrastructural study

1995 ◽  
Vol 52 (S1) ◽  
pp. 156-160 ◽  
Author(s):  
Lena N. Measures ◽  
Henry Hong
Keyword(s):  
Sea Water ◽  
Microscopic Analysis ◽  
Electron Microscopic ◽  
Free Living ◽  
Second Stage ◽  
Ultrastructural Evidence ◽  
Pseudoterranova Decipiens ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Transmission Electron Microscopic Analysis

The number of moults in the egg of sealworm, Pseudoterranova decipiens (Nematoda: Ascaridoidea), and other ascaridoids is contentious. Transmission electron microscopic analysis of eggs and free-living larvae of sealworm confirmed that only one moult occurs in the egg. The first-stage larval (L1) cuticle on embryos was first observed in eggs incubated at 15 °C in sea water on day 5 after eggs were dissected from the uterus of sealworm obtained from the stomach of grey seals. There was no ecdysis of this L1 cuticle. A second cuticle began to form beneath the L1 cuticle between day 5 and 12. The second-stage larval (L2) cuticle continued to develop and on day 12 the L2 larva hatched enclosed within the cuticle of the first stage. The L1 cuticle appeared to be partially resorbed during development of the L2 cuticle. This study provides the first ultrastructural evidence of the number of moults that occur in eggs of sealworm.

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