scholarly journals Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling

2019 ◽  
Vol 219 (1) ◽  
Author(s):  
Rachel L. Belote ◽  
Sanford M. Simon
Keyword(s):  
Cell Signaling ◽  
Neural Crest ◽  
Human Skin ◽  
Dendritic Spine ◽  
Culture System ◽  
Model System ◽  
Process Information ◽  
New Model ◽  
Secreted Factors ◽  
Dendritic Structures

Melanocytes are the neural crest–derived pigment-producing cells of the skin that possess dendrites. Yet little is known about how melanocyte dendrites receive and process information from neighboring cells. Here, using a co-culture system to interrogate the interaction between melanocyte dendrites and keratinocytes, we show that signals from neighboring keratinocytes trigger local compartmentalized Ca2+ transients within the melanocyte dendrites. The localized dendritic Ca2+ transients could be triggered by two keratinocyte-secreted factors, endothelin and acetylcholine, which acted via specific melanocyte receptors. Furthermore, compartmentalized Ca2+ transients were also generated on discrete dendritic spine-like structures on the melanocytes. These spines were also present in intact human skin. Our findings provide insights into how melanocyte dendrites communicate with neighboring cells and offer a new model system for studying compartmentalized signaling in dendritic structures.

scholarly journals Observation of direct vibrational excitation in gas-surface collisions of CO with Au(111): a new model system for surface dynamics

2013 ◽  
Vol 15 (6) ◽  
pp. 1863-1867 ◽  
Author(s):  
Tim Schäfer ◽  
Nils Bartels ◽  
Kai Golibrzuch ◽  
Christof Bartels ◽  
Hansjochen Köckert ◽  
...  
Keyword(s):  
Vibrational Excitation ◽  
Model System ◽  
Surface Dynamics ◽  
New Model

Attraction and penetration of Musa by the burrowing nematode, Radopholus similis, in an autotrophic bipartite culture system

Nematology ◽  
2021 ◽  
pp. 1-14
Author(s):  
Suganthagunthalam Dhakshinamoorthy ◽  
Erwin J. Galon ◽  
Annemie Elsen ◽  
Dirk De Waele
Keyword(s):  
Culture System ◽  
Model System ◽  
Radopholus Similis ◽  
Susceptible Plant ◽  
Compartment System ◽  
Female Migration ◽  
Equal Distance ◽  
Burrowing Nematode ◽  
Nematode Chemotaxis

Summary Our objective was to discover the stages (pre- or post-infection) in which the resistance to burrowing nematode (Radopholus similis) occurs in two resistant banana (Musa spp.) cultivars. An autotrophic in vitro culture system was used to compare R. similis migration towards, and penetration into, the banana roots. A new two-compartment autotrophic in vitro model system was developed using agar-based medium to examine the migration of R. similis to either the susceptible ‘Grande Naine’ or the resistant ‘Yangambi km5’ (‘Ykm5’), when both the Musa genotypes were present at equal distance. The autotrophic in vitro model system was advantageous, because it supported continuous root growth due to the actively photosynthesising shoots growing in the open air, while the in vitro root conditions make it possible to observe and assess the nematode chemotaxis in the transparent medium. Significantly fewer nematodes migrated towards the resistant ‘Ykm5’ plants when compared to both the susceptible ‘Grande Naine’, and another resistant cultivar, ‘Saba’, at 1 h after infection. This signals a possibility of a lower concentration or different composition of nematode attractants in ‘Ykm5’ root exudates. No significant differences were observed in the percentage of R. similis that migrated towards the roots of the susceptible and resistant banana plants at 3, 4 and 6 h after inoculation. No significant differences were observed in the percentages of female penetration in the resistant and susceptible plant roots at 1 and 2 days after inoculation. The results of the two-compartment system confirmed that when a choice is given to migrate towards the resistant and susceptible genotypes, no differences were observed in the percentage of female migration towards both the genotypes.

scholarly journals Establishment of a murine culture system for modeling the temporal progression of cranial and trunk neural crest cell differentiation

2018 ◽  
Vol 11 (12) ◽  
pp. dmm035097 ◽  
Author(s):  
Maria R. Replogle ◽  
Virinchipuram S. Sreevidya ◽  
Vivian M. Lee ◽  
Michael D. Laiosa ◽  
Kurt R. Svoboda ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Neural Crest ◽  
Culture System ◽  
Neural Crest Cell ◽  
Trunk Neural Crest ◽  
Crest Cell

Nanofaceted Platinum Surfaces:  A New Model System for Nanoparticle Catalysts

2005 ◽  
Vol 109 (49) ◽  
pp. 23543-23549 ◽  
Author(s):  
Vladimir Komanicky ◽  
Andreas Menzel ◽  
Kee-Chul Chang ◽  
Hoydoo You
Keyword(s):  
Model System ◽  
New Model ◽  
Nanoparticle Catalysts ◽  
Platinum Surfaces

A new model system for endometritis: Basic concepts and characterization of phenotypic and functional properties of bovine uterine neutrophils

1996 ◽  
Vol 46 (8) ◽  
pp. 1339-1356 ◽  
Author(s):  
H. Zerbe ◽  
H.-J. Schuberth ◽  
M. Hoedemaker ◽  
E. Grunert ◽  
W. Leibold
Keyword(s):  
Functional Properties ◽  
Model System ◽  
New Model ◽  
Basic Concepts
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