scholarly journals THE BASE OF THE PROXIMAL CONVOLUTED TUBULE CELLS OF RAT KIDNEY

1957 ◽  
Vol 3 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Helmut Ruska ◽  
Dan H. Moore ◽  
John Weinstock
Keyword(s):  
Hydrostatic Pressure ◽  
Capillary Pressure ◽  
Functional Unit ◽  
Rat Kidney ◽  
Three Dimensional ◽  
Blood Stream ◽  
Proximal Convoluted Tubule ◽  
Basal Labyrinth ◽  
Tubule Cells

The three dimensional arrangement of the compartments on the base of proximal convoluted tubule cells of rat kidney is described. An extracellular basal labyrinth is found to be enclosed by these compartments. The compartments with their mitochondria and the basal labyrinth are regarded as forming a functional unit. It is supposed that this basal unit serves for excretion of reabsorbed fluid from the cell into the labyrinth and for the development of hydrostatic pressure in the labyrinth to overcome the capillary pressure and to pass the reabsorbed fluid into the blood stream.

Dependence of ion fluxes on fluid transport by rat proximal tubule

1986 ◽  
Vol 250 (4) ◽  
pp. F680-F689 ◽  
Author(s):  
K. Bomsztyk ◽  
F. S. Wright
Keyword(s):  
Proximal Tubule ◽  
Fluid Transport ◽  
Rat Kidney ◽  
Water Flux ◽  
Fluid Secretion ◽  
Proximal Convoluted Tubule ◽  
Ion Fluxes ◽  
Fluid Absorption ◽  
Fluid Flux ◽  
K Transport

The effects of changes in transepithelial water flux (Jv) on sodium, chloride, calcium, and potassium transport by the proximal convoluted tubule were examined by applying a microperfusion technique to surface segments in kidneys of anesthetized rats. Perfusion solutions were prepared with ion concentrations similar to those in fluid normally present in the later parts of the proximal tubule. Osmolality of the perfusate was adjusted with mannitol. With no mannitol in the perfusates, net fluid absorption was observed. Addition of increasing amounts of mannitol first reduced Jv to zero and then reversed net fluid flux. At the maximal rates of fluid absorption, net absorption of Na, Cl, Ca, and K was observed. When Jv was reduced to zero, Na, Cl, and Ca absorption were reduced and K entered the lumen. Na, Cl, and Ca secretion occurred in association with the highest rates of net fluid secretion. The lumen-positive transepithelial potential progressively increased as the net fluid flux was reduced to zero and then reversed. The results demonstrate that changes in net water flux can affect Na, Cl, Ca, and K transport by the proximal convoluted tubule of the rat kidney. These changes in net ion fluxes are not entirely accounted for by changes in bulk-phase transepithelial electrochemical gradients.

Mechanism of volume replacement and vascular constriction following hemorrhage

1965 ◽  
Vol 208 (1) ◽  
pp. 169-181 ◽  
Author(s):  
F. J. Haddy ◽  
J. B. Scott ◽  
J. I. Molnar
Keyword(s):  
Hydrostatic Pressure ◽  
Capillary Pressure ◽  
Blood Viscosity ◽  
Perfusion Pressure ◽  
Vascular System ◽  
Volume Replacement ◽  
Right Atrial ◽  
Tissue Fluid ◽  
Partial Recovery ◽  
Local Regulation

Experiments in the anesthetized dog suggest that fluid moves into the vascular system following hemorrhage because of fall in capillary hydrostatic pressure subsequent to decrease in aortic and right atrial pressures and increase in precapillary resistance. Postcapillary resistance also rises but not sufficiently to prevent the fall in capillary pressure. The increase in precapillary resistance results from active constriction, passive constriction, and, very transiently, increase in blood viscosity. The active constriction of precapillary vessels is due to a baroreceptor-induced sympathico-adrenal discharge and to some nonadrenal, nonrenal circulating vasoconstrictor substance. The active vasoconstriction is not well maintained in forelimb, intestine, or kidney because of local regulation ("autoregulation") and baroreceptor-induced lessening of the sympathico-adrenal discharge subsequent to movement of tissue fluid into the vascular system. This waning constriction together with a rise in perfusion pressure permits partial recovery of flow. The increase in forelimb postcapillary resistance results mainly from passive constriction. Active constriction subsequent to the sympathico-adrenal discharge is seen only with large hemorrhage.

Immunolocalization of NHE8 in rat kidney

2005 ◽  
Vol 288 (3) ◽  
pp. F530-F538 ◽  
Author(s):  
Sunita Goyal ◽  
SueAnn Mentone ◽  
Peter S. Aronson
Keyword(s):  
Proximal Tubule ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Surface Membrane ◽  
Proximal Tubules ◽  
Intense Staining ◽  
Proximal Tubule Cells ◽  
Nephron Segment ◽  
Tubule Cells

In situ hybridization studies demonstrated that Na+/H+ exchanger NHE8 is expressed in kidney proximal tubules. Although membrane fractionation studies suggested apical brush-border localization, precise membrane localization could not be definitively established. The goal of the present study was to develop isoform-specific NHE8 antibodies as a tool to directly establish the localization of NHE8 protein in the kidney by immunocytochemistry. Toward this goal, two sets of antibodies that label different NHE8 epitopes were developed. Monoclonal antibody 7A11 and polyclonal antibody Rab65 both specifically labeled NHE8 by Western blotting as well as by immunofluorescence microscopy. The immunolocalization pattern in the kidney seen with both antibodies was the same, thereby validating NHE8 specificity. In particular, NHE8 expression was observed on the apical brush-border membrane of all proximal tubules from S1 to S3. The most intense staining was evident in proximal tubules in the deeper cortex and medulla with a significant but somewhat weaker staining in superficial proximal tubules. Colocalization studies with γ-glutamyltranspeptidase and megalin indicated expression of NHE8 on both the microvillar surface membrane and the coated-pit region of proximal tubule cells, suggesting that NHE8 may be subject to endocytic retrieval and recycling. Although colocalizing in the proximal tubule with NHE3, no significant alteration in NHE8 protein expression was evident in NHE3-null mice. We conclude that NHE8 is expressed on the apical brush-border membrane of proximal tubule cells, where it may play a role in mediating or regulating ion transport in this nephron segment.

Inverse modeling of transient three-dimensional core-scale two-phase flows

2021 ◽  
Author(s):  
Andrea Manzoni ◽  
Aronne Dell'Oca ◽  
Martina Siena ◽  
Alberto Guadagnini
Keyword(s):  
Capillary Pressure ◽  
Random Noise ◽  
Three Dimensional ◽  
Population Based ◽  
Model Parameters ◽  
Reference Scenario ◽  
Two Phase ◽  
De Algorithm ◽  
Continuum Scale ◽  
Available Information

<p>We consider transient three-dimensional (3D) two-phase (oil and water) flows, taking place at the core-scale. In this context, we aim at exploiting the full information content associated with available information of (i) the 3D distribution of oil saturation and (ii) the overall pressure difference across the rock sample, to estimate the set of model parameters. We consider a continuum-scale description of the system behavior upon relying on the widely employed Brooks-Corey model for the characterization of relative permeabilities and on the capillary pressure correlation introduced by Skjaeveland et al. (2000). To provide a transparent way of assessing the results of the inversion, we rely on a synthetic reference scenario. The latter is intended to mimic having at our disposal 3D and section-averaged distributions of (time-dependent) oil saturations of the kind that can be acquired during typical laboratory experiments. These are in turn corrupted by way of a random noise, to address the influence of experimental uncertainties. We focus on diverse scenarios encompassing imbibition and drainage conditions. We employ two population-based optimization algorithms, i.e., (i) the particle swarm optimization (PSO); and (ii) the differential evolution (DE), which enable one to effectively tackle the high-dimensionality parameters space (i.e., 12 dimensions in our setting) we consider. Model calibration results are of satisfactory quality for the majority of the tested scenarios, whereas the DE algorithm is associated with highest effectiveness.</p><p><strong>References</strong></p><p>S.M. Skjaeveland; L.M. Siqveland; A. Kjosavik; W.L. Hammervold Thomas; G.A. Virnovsky (2000). Capillary Pressure Correlation for Mixed-Wet Reservoirs SPE Res Eval & Eng 3 (01): 60–67. https://doi.org/10.2118/60900-PA</p>

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