scholarly journals ALLERGIC INFLAMMATION

1963 ◽  
Vol 118 (4) ◽  
pp. 557-564 ◽  
Author(s):  
Henry Z. Movat ◽  
Neil V. P. Fernando ◽  
Tsuneo Uriuhara ◽  
William J. Weiser
Keyword(s):  
Fine Structure ◽  
Microscopic Examination ◽  
Allergic Inflammation ◽  
Electron Microscopic Examination ◽  
Collagen Fibrils ◽  
Electron Microscopic ◽  
Ultrastructural Evidence ◽  
Antigen Antibody

The fine structure of collagen fibrils at sites of antigen-antibody interaction is described. Following injection of antigen (BSA or ferritin) into the center of the cornea of hyperimmune rabbits, an acidophilic ring of precipitate forms at the periphery of the cornea, where antigen and antibody interact in optimal proportions. The precipitate is soon removed by infiltrating polymorphs, but persists longer in leukopenic animals. Electron microscopic examination of the cornea showed no alteration of the collagen fibrils in the area of antigen-antibody precipitation or in the remaining cornea. Contrary to many claims there was no swelling, fragmentation, or disintegration of the fibrils and they had a normal periodicity. Polymorphs infiltrated the precipitates and phagocytosed the antigen-antibody complexes. There was some ultrastructural evidence that degradation of the complexes took place in the polymorphs.

scholarly journals Splenic Macrophages in Thrombocytopenia

Blood ◽  
1969 ◽  
Vol 33 (2) ◽  
pp. 240-245 ◽  
Author(s):  
B. G. FIRKIN ◽  
R. WRIGHT ◽  
S. MILLER ◽  
E. STOKES
Keyword(s):  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Splenic Tissue ◽  
Electron Microscopic ◽  
Thrombocytopenic Purpura ◽  
Hematologic Disorders ◽  
Ultrastructural Evidence ◽  
Splenic Macrophages ◽  
Intracellular Digestion ◽  
Morphologic Characteristics

Abstract Electron microscopic examination of splenic tissue obtained from patients with a variety of hematologic disorders has shown ultrastructural evidence for platelet breakdown within splenic histiocytes in seven out of eight patients with idiopathic thrombocytopenic purpura (I. T. P.). The morphologic characteristics of this intracellular digestion have been described.

Variation in Eyespot Ultrastructure in Chlamydomonas Reinhardi (ac-31)

1974 ◽  
Vol 15 (3) ◽  
pp. 481-494
Author(s):  
HELEN E. GRUBER ◽  
BENJAMIN ROSARIO
Keyword(s):  
Fine Structure ◽  
Microscopic Examination ◽  
Sodium Acetate ◽  
Minimal Medium ◽  
Electron Microscopic Examination ◽  
Carotenoid Pigments ◽  
Electron Microscopic ◽  
Morphological Variations ◽  
Dense Bodies ◽  
Pale Green

Several morphological variations in eyespot complex fine structure were exhibited in some cells of the pale-green mutant strain ac-31 of Chlamydomonas reinhardi. The cells were grown in minimal medium supplemented with 0.2 % sodium acetate and were harvested by centrifugation and prepared for electron-microscopic examination. Microtubules were seen near the flagella, confirming previous observations. Microtubules were also seen near the eyespot complex. Although a direct connexion between the microtubules of the flagellar and eyespot region was never observed, this does not exclude the possibility that it exists and thus provides a structural and functional connexion between the two organelles. Occasionally irregular curved bodies intimately associated with the eyespot complex of some cells appeared to displace the chloroplast and cell membranes. These bodies often appeared to be nearly covered by a limiting membrane and were found near empty ‘cavities’ in the eyespot plate. Crystalline arrays of dense bodies were observed in some sections. The significance of these bodies is discussed in relation to the functional state of the carotenoid pigments making up the eyespot granules. An hypothesis for the formation of the rod-like structures is presented, based upon the observation of granules which had fused together to form a helix.

scholarly journals The Fine Structure and Biosynthesis of Silk Fibroin

1952 ◽  
Vol 5 (3) ◽  
pp. 366 ◽  
Author(s):  
EH Mercer
Keyword(s):  
Fine Structure ◽  
Silk Fibroin ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Fibre Axis ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
X Ray

Electron microscopic examination of fibrillar fragments produced by the enzymic disintegration of silk fibroin suggests the existence of fine microfibrils about 100 A in diameter extended parallel to the length of the fibre axis. The microfibrils are similar in width to the crystalline micelles deduced from X-ray diffraction.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Artifact-free electron microscopic immunocytochemistry on rat brain tissue

1991 ◽  
Vol 49 ◽  
pp. 272-273
Author(s):  
Veronika Burmeister ◽  
N. Ludvig ◽  
P.C. Jobe
Keyword(s):  
Microscopic Examination ◽  
Free Electron ◽  
Electron Microscopic Examination ◽  
Ultrastructural Localization ◽  
Rabbit Serum ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Phosphate Buffered Saline ◽  
Rat Brain Tissue ◽  
Immune Rabbit

Electron microscopic immunocytochemistry provides an important tool to determine the ultrastructural distribution of various molecules in both normal and pathologic tissues. However, the specific immunostaining may be obscured by artifactual immunoreaction product, misleading the investigator. Previous observations show that shortening the incubation period with the primary antibody from the generally used 12-24 hours to 1 hour substantially reduces the artifactual immunostaining. We now extend this finding by the demonstration of artifact-free ultrastructural localization of the Ca2/calmodulindependent cyclic nucleotide phosphodiesterase (CaM-dependent PDE) immunoreactivity in brain.Anesthetized rats were perfused transcardially with phosphate-buffered saline followed by a fixative containing paraformaldehyde (4%) and glutaraldehyde (0.25%) in PBS. The brains were removed, and 40μm sections were cut with a vibratome. The sections were processed for immunocytochemistry as described by Ludvig et al. Both non-immune rabbit serum and specific CaM-dependent PDE antibodies were used. In both experiments incubations were at one hour and overnight. The immunostained sections were processed for electron microscopic examination.

Ultrastructural studies of Chlamydia psittaci 6BC variant strains. I. Ultrastructure of the surface layers of egg-passaged 6BC strain

1973 ◽  
Vol 19 (8) ◽  
pp. 887-894
Author(s):  
Linda Poffenroth ◽  
J. W. Costerton ◽  
Nonna Kordová ◽  
John C. Wilt
Keyword(s):  
Chick Embryo ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Chlamydia Psittaci ◽  
Gram Negative Bacteria ◽  
Surface Layers ◽  
Electron Microscopic ◽  
Gram Negative ◽  
Ultrastructural Studies ◽  
First Time

Electron microscopic examination of a semipurified Chlamydia psittaci 6BC strain attenuated in chick embryo yolk sac revealed for the first time two morphologically distinct small elementary bodies which differ both in the ultrastructure of their surface layers and in their buoyant densities in sucrose gradients. Also, the morphology of the surface layers of the larger reticulate forms in cell-free systems is described for the first time. Many points of difference between the surface envelopes and internal structure of chlamydial particles and those of Gram-negative bacteria are discussed.

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