scholarly journals A STUDY OF THE ANTIGENS INVOLVED IN ADENOVIRUS 12 TUMORIGENESIS BY IMMUNODIFFUSION TECHNIQUES

1965 ◽  
Vol 121 (6) ◽  
pp. 955-967 ◽  
Author(s):  
Leonard D. Berman ◽  
Wallace P. Rowe
Keyword(s):  
Tumor Antigen ◽  
Tumor Antigens ◽  
Neutralizing Antibody ◽  
Classical Type ◽  
Agar Gel ◽  
Culture Cells ◽  
Diffusion Technique ◽  
Antibody Titers ◽  
Infectious Cycle ◽  
Gel Diffusion

The use of the agar gel diffusion technique has established the presence of three distinct antigenic reactions in the sera of Ad. 12 tumor-bearing hamsters. Only one of these antigens is directly demonstrable in the tumor. This "tumor" antigen is also formed during early stages of the infectious cycle in tissue culture cells. Other antigens present in the tumor, but only demonstrable indirectly with the use of antibody-containing serum of tumored hamsters, are the classical type-specific C antigen, and a new antigen, termed D. Of ninety-eight Ad. 12 tumored hamster sera, six reacted in gel diffusion with virus and tumor preparations, and thirty-one with tumor only. Sera which reacted in gel diffusion with viral antigen uniformly bad neutralizing antibody and high titers of CF antibody against viral and tumor antigens; however, many sera with comparable antibody titers did not react with the virus in gel diffusion. Sera which reacted in gel diffusion only with tumor antigen also had high CF antibody titers, but there was no correlation with neutralizing antibody.

The Separation of Insect Haemolymph Proteins

1964 ◽  
Vol 96 (1-2) ◽  
pp. 110-110
Author(s):  
B. G. Loughton ◽  
P. Rueffel ◽  
H. Stich ◽  
A. S. West
Keyword(s):  
Amino Acid ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Homologous Proteins ◽  
Agar Gel ◽  
Diffusion Technique ◽  
Starch Gel ◽  
Rapid Characterization ◽  
Gel Diffusion

It has been suggested that information on the phylogenetic relationships of genera and species could be obtained by comparing the amino acid sequence in the homologous proteins of different species. This procedure is extremely difficult and time-consuming.However, a relatively rapid characterization of proteins can be obtained by analysing their mobilities with starch-gel electrophoresis and examination of antigenic diversity by the agar gel diffusion technique of Ouchterlony.

An Agar-Gel Diffusion Technique for demonstrating Antibody Production by Lymph Node Cells in Tissue Culture

Nature ◽  
1962 ◽  
Vol 196 (4850) ◽  
pp. 183-184 ◽  
Author(s):  
RICHARD H. EGDAHL ◽  
HERTHA CRESS ◽  
JOHN A. MANNICK
Keyword(s):  
Tissue Culture ◽  
Lymph Node ◽  
Antibody Production ◽  
Agar Gel ◽  
Diffusion Technique ◽  
Lymph Node Cells ◽  
Gel Diffusion

scholarly journals Arbovirus Identification by an Agar-Gel Diffusion Technique

1970 ◽  
Vol 19 (4) ◽  
pp. 557-564 ◽  
Author(s):  
C. H. Calisher ◽  
K. S. C. Maness
Keyword(s):  
Agar Gel ◽  
Diffusion Technique ◽  
Gel Diffusion

Stability of Five Antibiotics

1980 ◽  
Vol 14 (12) ◽  
pp. 848-850 ◽  
Author(s):  
Elizabeth Chow Tung ◽  
Ernest L. Gurwich ◽  
Joseph A. Sula ◽  
Michael Kodack
Keyword(s):  
Sodium Chloride ◽  
Sodium Bicarbonate ◽  
Normal Saline ◽  
Room Temperature ◽  
Agar Gel ◽  
Diffusion Technique ◽  
The Stability ◽  
Aseptic Conditions ◽  
Erythromycin Lactobionate ◽  
Gel Diffusion

The stability of methicillin, amikacin, erythromycin lactobionate, vancomycin, and ticarcillin in plastic intravenous containers of sodium chloride injection 0.9%, USP and dextrose 5% injection, USP was studied. The study was conducted under aseptic conditions for a period of 24 hours. The samples, drawn at various times, were assayed by a modified agar gel diffusion technique. The study solutions were stored at room temperature. This study revealed that amikacin, methicillin, vancomycin, and ticarcillin are stable for 24 hours in the test solutions. Erythromycin lactobionate is stable for 24 hours in normal saline, but the addition of sodium bicarbonate 4% (Neut®) was necessary to assure stability in dextrose 5 %.

SEROLOGICAL AND PHYSICAL PROPERTIES OF SOME STONE-FRUIT VIRUSES: I. PREPARATIVE AND SEROLOGICAL TECHNIQUES

1959 ◽  
Vol 37 (6) ◽  
pp. 1157-1165 ◽  
Author(s):  
R. S. Willison ◽  
M. Weintraub ◽  
J. H. Tremaine
Keyword(s):  
Particle Size ◽  
Absorption Spectrum ◽  
Sedimentation Rate ◽  
Virus Antigen ◽  
Ultraviolet Absorption Spectrum ◽  
Differential Centrifugation ◽  
Agar Gel ◽  
Diffusion Technique ◽  
Homologous Antiserum ◽  
Gel Diffusion

Strain Y.4 of the cherry yellows virus was purified from cucumber by four methods, which differed mainly at the clarification stage: A, the homogenate buffered at pH 6.5 was frozen; B, leaves were frozen and the expressed sap was buffered at pH 5.0; C, fresh expressed sap was diluted with pH 5.0 buffer; D, fresh expressed sap was dialyzed against.05 M acetate buffer at pH 4.5. One or two cycles of differential centrifugation were applied after clarification. Freezing usually inactivated the virus. Methods C and D yielded infective preparations. Ultraviolet absorption spectrum analysis, and particle size and sedimentation rate determinations, supported by infectivity tests, indicated that, in these infective preparations, the virus replaced most if not all of the components found in comparable extracts from healthy sources. The agar gel diffusion technique was used for precipitin tests. Virus antigen from cucumber sources reacted with homologous antiserum or with antiserum derived from infected cherry petals and vice versa.

IMMUNOCHEMICAL PROPERTIES OF THYROID PROTEINS

1964 ◽  
Vol 42 (8) ◽  
pp. 1169-1176 ◽  
Author(s):  
L. Perelmutter ◽  
N. R. Stephenson
Keyword(s):  
Agar Gel ◽  
Extinction Coefficients ◽  
Diffusion Technique ◽  
Thyroid Glands ◽  
Sufficient Concentration ◽  
Gel Diffusion

Saline extracts obtained from both beef and hog thyroid glands were chromatographed on Sephadex G-200. An ultracentrifugal and immunochemical examination of the fractions showed that subfractions which possessed the 19 S component and a sufficient concentration of the 25 S component gave rise to two precipitin bands by the agar gel diffusion technique. Immunoelectrophoretic studies indicated that these antigens migrated in the region of α2 globulins. In addition, it was found that subfractions which contained both the 19 S and 25 S components had higher extinction coefficients at 280 mμ and lower OD280/OD260 ratios than those in which the 19 S component occurred alone. From these results, it can be suggested that the 19 S and 25 S components have different immunochemical and spectrophotometric properties.

Sign in / Sign up

Export Citation Format

Share Document