scholarly journals INHIBITION OF RELEASE OF VACCINIA VIRUS BY N1-ISONICOTINOYL-N2-3-METHYL-4-CHLOROBENZOYLHYDRAZINE

1969 ◽  
Vol 129 (4) ◽  
pp. 795-808 ◽  
Author(s):  
Nobuo Kato ◽  
Hans J. Eggers ◽  
Heinrich Rolly
Keyword(s):  
Chick Embryo ◽  
Vaccinia Virus ◽  
Inhibitory Effect ◽  
Virus Multiplication ◽  
Kidney Cells ◽  
Virus Release ◽  
Single Cycle ◽  
Infected Cells ◽  
Embryo Fibroblasts ◽  
Intracellular Virus

N1-isonicotinoyl-N2-3-methyl-4-chlorobenzoylhydrazine (IMCBH) is a selective inhibitor of vaccinia virus multiplication. In concentrations up to 50 µg/ml, IMCBH causes neither toxic morphologic changes, nor does it inhibit the multiplication of cells. Viruses other than vaccinia are not affected by IMCBH. The virus-inhibitory effect of IMCBH is dependent on the type of host cell used, i.e., the compound is effective in chick embryo fibroblasts and monkey kidney cells but not in L cells. IMCBH does not exhibit any protecting effect on vaccinia virus-infected mice or rabbits. IMCBH interferes with virus release: in single cycle experiments in chick embryo fibroblasts, IMCBH strongly blocks the release of vaccinia virus at concentrations as low as 3 µg/ml, while intracellular virus synthesis is hardly affected. Viral cytopathic changes are completely suppressed by IMCBH within the span of a single cycle infection, although extensive changes eventually occur. By inhibiting virus release from initially infected cells, IMCBH markedly inhibits the multiplication of vaccinia virus in cell cultures infected at low virus/ cell multiplicities. IMCBH does not inhibit the early toxic cytopathic changes induced by large inocula of vaccinia virus in BHK21 cells.

scholarly journals The effects of UK 2054 on the multiplication of influenza viruses

1970 ◽  
Vol 68 (1) ◽  
pp. 151-158 ◽  
Author(s):  
R. D. Barry ◽  
Patricia Davies
Keyword(s):  
Influenza Virus ◽  
Chick Embryo ◽  
Inhibitory Effect ◽  
Virus Multiplication ◽  
Influenza Viruses ◽  
Host Cells ◽  
Virus Infectivity ◽  
Virus Growth ◽  
Virus Particles ◽  
Embryo Fibroblasts

SummaryThe isoquinoline compound UK 2054 prevents the uptake of influenza virus by susceptible cells. Pre-incubation of virus particles with 500μg./ml. UK 2054 at 37°C. for 2 hr. does not reduce virus infectivity. Host cells vary in their responsiveness to the inhibitory effect of UK 2054; virus multiplication is inhibited in chick allantoic cells by lower concentrations than those required to inhibit virus growth in chick embryo fibroblasts. The effectiveness of UK 2054 is reduced by the presence of serum.It is concluded that inhibition of influenza virus multiplication by UK2054 might result from interaction of the inhibitor with both virus and cells. Any direct combination between inhibitor and virus is completely reversible.

scholarly journals Mushrooms as a source of substances with antiviral activity

2014 ◽  
Vol 16 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Martyna Kandefer-Szerszeń ◽  
Zbigniew Kawecki ◽  
Bogusław Sałata ◽  
Maria Witek
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Antiviral Activity ◽  
Vaccinia Virus ◽  
Fungal Species ◽  
Water Extracts ◽  
Boletus Edulis ◽  
Vaccinia Viruses ◽  
Embryo Fibroblasts ◽  
Armillariella Mellea

Water extracts the fructifications of 56 species of fungi were examined as a source of antiviral substances with activity against VS and vaccinia viruses. Extracts from 16 fungal species exhibited the antiviral activity. Water extracts from <i>Boletus edulis</i> active against vaccinia virus and extract from <i>Armillariella mellea</i> active against VS virus are particularly worth nothing. Both of them in applied concentrations were not toxic in chick embryo fibroblasts tissue culture.

Reduced steady-state levels of vaccinia virus-specific early mRNAs in interferon-treated chick embryo fibroblasts

Virology ◽  
1987 ◽  
Vol 158 (1) ◽  
pp. 28-33 ◽  
Author(s):  
J. Grün ◽  
E. Kroon ◽  
B. Zoller ◽  
U. Krempien ◽  
C. Jungwirth
Keyword(s):  
Steady State ◽  
Chick Embryo ◽  
Vaccinia Virus ◽  
Steady State Levels ◽  
Embryo Fibroblasts

scholarly journals THE INHIBITORY EFFECT OF POLYSACCHARIDE ON MUMPS VIRUS MULTIPLICATION

1948 ◽  
Vol 87 (5) ◽  
pp. 385-410 ◽  
Author(s):  
Harold S. Ginsberg ◽  
Walther F. Goebel ◽  
Frank L. Horsfall
Keyword(s):  
Chick Embryo ◽  
Inhibitory Effect ◽  
Virus Multiplication ◽  
Mumps Virus ◽  
Viral Multiplication ◽  
Chemical Studies ◽  
Structural Configurations ◽  
Serological Activity

Polysaccharides derived from type-specific Friedländer bacilli cause inhibition of the multiplication of mumps virus in the allantoic sac of the chick embryo. As little as 5 µg. of polysaccharide is effective as an inhibitor. Inhibition of multiplication is obtained when polysaccharide is injected as long as 4 days after inoculation of virus. Chemical studies have shown that the structural configurations of the polysaccharide responsible for specific serological activity are not identical with those which determine the inhibitory effect relative to mumps virus. The possible mechanisms of the inhibition of viral multiplication by means of polysaccharides are discussed.

Regulation of histone H5 and H1° gene expression under the control of vaccinia virus-specific sequences in interferon-treated chick embryo fibroblasts

Virology ◽  
1991 ◽  
Vol 180 (2) ◽  
pp. 535-542 ◽  
Author(s):  
Joachim Grün ◽  
Ingrid Redmann-Müller ◽  
Doris Blum ◽  
Hans-Joachim Degen ◽  
Detlef Doenecke ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chick Embryo ◽  
Vaccinia Virus ◽  
Embryo Fibroblasts ◽  
Histone H5 ◽  
Specific Sequences

scholarly journals INDUCTION OF SKIN RESISTANCE TO VACCINIA VIRUS IN RABBITS BY VACCINIA-SOLUBLE EARLY ANTIGENS

1973 ◽  
Vol 138 (5) ◽  
pp. 1033-1043 ◽  
Author(s):  
Y. Ueda ◽  
I. Tagaya
Keyword(s):  
Vaccinia Virus ◽  
Skin Resistance ◽  
Neutralizing Antibody ◽  
Skin Lesions ◽  
Virus Multiplication ◽  
Lethal Mutant ◽  
Late Protein ◽  
Infected Cells ◽  
Conditional Lethal Mutant

The immunological role of vaccinia-soluble early antigen was examined in rabbits. The antigens were prepared from HeLa cells infected with a conditional lethal mutant of vaccinia virus, which induces in these cells early antigens including those responsible for surface immunofluorescence of infected cells, but not viral DNA and late protein syntheses. Immunization of rabbits with the antigens in Freund's complete adjuvant induced complement-fixing antibody but neither detectable circulating neutralizing antibody nor any detectable level of inhibitory substance or interferon in the skin of the animals. When immunized animals were inoculated intradermally with vaccinia virus, multiplication of virus in the skin was greatly inhibited, being accompanied by an earlier appearance as well as an accelerated wane of the local reactions. The resistance could not be transferred passively by the serum of immunized animals to normal rabbits. Immunization of rabbits with the antigens without the adjuvant not only failed to inhibit but, contrariwise, enhanced the multiplication of intradermally inoculated vaccinia virus, inducing heavy skin lesions and exalted virus multiplication.

scholarly journals Effect of 5‘-deoxy-5’-isobutylthioadenosine on putrescine uptake and polyamine biosynthesis by chick embryo fibroblasts

1982 ◽  
Vol 204 (3) ◽  
pp. 853-859 ◽  
Author(s):  
F Lawrence ◽  
U Bachrach ◽  
M Robert-Géro
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Inhibitory Effect ◽  
Polyamine Biosynthesis ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

The effect of 5‘-deoxy-5’-S-isobutylthioadenosine (SIBA) on polyamine biosynthesis has been studied by using cultured chick embryo fibroblasts. It has been shown that the drug inhibits the uptake of [14C]putrescine and its conversion into labelled spermidine or spermine. The inhibitory effect is reversed by removing the inhibitor after exposing the cells to the drug for 24 h. SIBA also caused a significant decrease in cellular spermine levels and an accumulation of putrescine. These changes are reversed by removing the inhibitor. SIBA had the same effect on chick embryo fibroblasts transformed by Rous sarcoma virus; a decrease in cellular spermine levels in SIBA-treated cells was observed. In all the experiments SIBA caused a reduction in the spermine/putrescine and spermidine/putrescine ratios. It is suggested that SIBA is not only an inhibitor of transmethylation but also interferes with polyamine biosynthesis, probably by blocking aminopropyltransferase.

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