scholarly journals EFFECT OF RECENT ANTIGEN PRIMING ON ADOPTIVE IMMUNE RESPONSES

1974 ◽  
Vol 139 (1) ◽  
pp. 1-12 ◽  
Author(s):  
J. Sprent ◽  
J. F. A. P. Miller
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Mesenteric Lymph Node ◽  
Antibody Responses ◽  
The Other ◽  
Spleen Cells ◽  
Antigen Concentration ◽  
Short Term ◽  
Mesenteric Lymph ◽  
Circulating Lymphocytes

When thoracic duct lymphocytes (TDL) or mesenteric lymph node (MLN) cells from mice primed 1 day before with either sheep erythrocytes (SRC) or horse erythrocytes (HRC) were transferred together with both SRC and HRC to irradiated mice, antibody responses measured 7 days later could not be detected to the priming antigen but were high to the other antigen. Furthermore, this unresponsiveness of TDL and MLN to the priming antigen could not be abrogated by delaying antigen challenge of the transferred cells for 1–2 wk. Previous work had shown that short-term priming with antigen also induced specific unresponsiveness in spleen cells on adoptive transfer. Unresponsiveness in these cells, however, was only of temporary duration, full recovery in the reactivity of the cells being observed when challenge with the priming antigen on transfer was delayed for 5 or more days. Since the present work showed that such recovery from initial unresponsiveness on transfer was unique to spleen cells and did not apply to TDL or MLN, it appeared that different mechanisms were responsible for the unresponsiveness in the three populations. It is proposed that the unresponsiveness detected in TDL and MLN cells in the present study resulted from a deficiency of antigen-reactive cells, these cells having been recruited to the spleen, i.e., a region of antigen concentration. This concept of antigen-induced selective recruitment of circulating lymphocytes was supported by evidence that 51Cr-labeled heterologous erythrocytes indeed localized largely in the spleen after intravenous injection but not in MLN.

Specific cross-immunity between Trichinella spiralis and Trichuris muris: immunization with heterologous infections and antigens and transfer of immunity with heterologous immune mesenteric lymph node cells

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 381-389 ◽  
Author(s):  
T. D. G. Lee ◽  
R. K. Grencis ◽  
D. Wakelin
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Primary Infection ◽  
Trichinella Spiralis ◽  
The Other ◽  
Trichuris Muris ◽  
Mesenteric Lymph ◽  
Heterologous Challenge ◽  
Lymph Node Cells ◽  
Cross Immunity

SUMMARYInfections with either 300 infective Trichinella spiralis larvae or 400 embryonated eggs of Trichuris muris were effective in eliciting accelerated expulsion of heterologous challenge infections given 20 days after the primary infection. Accelerated expulsion could also be achieved by the administration of soluble crude worm antigen given 12 days prior to heterologous challenge or by adoptive transfer of mesenteric lymph node cells taken from mice infected with the heterologous parasite. Each species is capable of eliciting an accelerated secondary expulsion response in hosts that have been actively or adoptively immunized against the other species and these results are taken to indicate that there is a specific cross-immunity between T. spiralis and T. muris due to shared antigens. It is postulated that these shared antigens are derived from stichocyte granules.

scholarly journals Recombinant Proteins of Cryptosporidium parvum Induce Proliferation of Mesenteric Lymph Node Cells in Infected Mice

2005 ◽  
Vol 73 (8) ◽  
pp. 5245-5248 ◽  
Author(s):  
Inderpal Singh ◽  
Cynthia Theodos ◽  
Saul Tzipori
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Recombinant Proteins ◽  
Cryptosporidium Parvum ◽  
Mesenteric Lymph Node ◽  
Recombinant Antigens ◽  
Cellular Immune Responses ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Cellular Immune

ABSTRACT Recombinant antigens of Cryptosporidium parvum, Cp900 and Cp40 but not Cp15, stimulated C. parvum-specific proliferative immune responses of mesenteric lymph node cells in C57BL/6J mice infected with different isolates (MD, GCH1, UCP, and IOWA) of C. parvum, indicating that both Cp900 and Cp40 are immunodominant targets of cellular immune responses during C. parvum infection.

scholarly journals Leptin and adiponectin supplementation modifies mesenteric lymph node lymphocyte composition and functionality in suckling rats

2018 ◽  
Vol 119 (5) ◽  
pp. 486-495 ◽  
Author(s):  
Blanca Grases-Pintó ◽  
Mar Abril-Gil ◽  
Maria J. Rodríguez-Lagunas ◽  
Margarida Castell ◽  
Francisco J. Pérez-Cano ◽  
...  
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Mesenteric Lymph Node ◽  
Early Life ◽  
Suckling Period ◽  
Mesenteric Lymph Nodes ◽  
Trophic Effect ◽  
Mesenteric Lymph ◽  
Intestinal Immune System ◽  
Intestinal Iga

AbstractAt birth, when immune responses are insufficient, there begins the development of the defence capability against pathogens. Leptin and adiponectin, adipokines that are present in breast milk, have been shown to play a role in the regulation of immune responses. We report here, for the first time, the influence of in vivo adipokine supplementation on the intestinal immune system in early life. Suckling Wistar rats were daily supplemented with leptin (0·7 μg/kg per d, n 36) or adiponectin (35 μg/kg per d, n 36) during the suckling period. The lymphocyte composition, proliferation and cytokine secretion from mesenteric lymph node lymphocytes (on days 14 and 21), as well as intestinal IgA and IgM concentration (day 21), were evaluated. At day 14, leptin supplementation significantly increased the TCRαβ+ cell proportion in mesenteric lymph nodes, in particular owing to an increase in the TCRαβ+ CD8+ cell population. Moreover, the leptin or adiponectin supplementation promoted the early development CD8+ cells, with adiponectin being the only adipokine capable of enhancing the lymphoproliferative ability at the end of the suckling period. Although leptin decreased intestinal IgA concentration, it had a trophic effect on the intestine in early life. Supplementation of both adipokines modulated the cytokine profile during (day 14) and at the end (day 21) of the suckling period. These results suggest that leptin and adiponectin during suckling play a role in the development of mucosal immunity in early life.

Mesenteric lymph node stroma cells in the generation of intestinal immune responses

2009 ◽  
Vol 87 (10) ◽  
pp. 945-951 ◽  
Author(s):  
Oliver Pabst ◽  
Benjamin Wahl ◽  
Günter Bernhardt ◽  
Swantje I. Hammerschmidt
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Mesenteric Lymph Node ◽  
Mesenteric Lymph

scholarly journals CD markers of camel (Camelusdromedarius) intestine naturally infected with Mycobacteriumavium subsp. paratuberculosis: Distinct expression of Madcam-1 and CX3CR1

2020 ◽  
Vol 44 (5) ◽  
pp. 1010-1023
Author(s):  
Saeed AL-RAMADAN ◽  
Kazem AL-MOHAMMED SALEM ◽  
Ibrahim ALSHUBAITH ◽  
Ahmed ALLUWAIMI
Keyword(s):  
Lymph Node ◽  
Immune Responses ◽  
Lamina Propria ◽  
Mesenteric Lymph Node ◽  
Immunohistochemical Staining ◽  
Vital Role ◽  
High Expression ◽  
Macrophage Phenotype ◽  
Mesenteric Lymph ◽  
Cd Markers

Mycobacteriumavium subsp. paratuberculosis (MAP) infection in camel requires extensive research, particularly the immune responses in the intestine. This study aimed to investigate the nature of the cellular populations that are driven by the immunopathological responses in the camel intestine infected with MAP at different ages. Immunohistochemical staining was carried out on tissues obtained from naturally infected young (5–10 years old) and older (12–15 years old) camels. The staining of the tissues, ileum, mesenteric lymph node, jejunum, and supramammary lymph nodes, with anti-CD3+, CD4+, CD8+, CD25+, CD11c+, CD14+, WC1+, CX3CR1, and Madcam-1 monoclonal antibodies revealed high expression of the molecules CD8+, CD25+, CD11c+, CD14+, WC1+, CX3CR1, and Madcam-1 in the ileum and mesenteric lymph node of the infected older camels. The results indicated the recruitment of CD8+lymphocytes, CD14+ macrophages, and CD11c+ dendritic cells to the ileal lamina propria. High expression of CX3CR1 could indicate a vital role for this special macrophage phenotype in the ileal lamina propria in maintaining intestinal homeostasis. Madcam-1 expression could have an essential role in defining the nature of the recruited cells to the site of the infection. Expression of CX3CR1 and Madcam-1 is a novel finding that merits further attention and pursuit to reveal their significance in the immune responses to MAP in the camel’s intestine.

Immune responses of graft mesenteric lymph node in small bowel transplantation

2004 ◽  
Vol 116 (2) ◽  
pp. 269-276 ◽  
Author(s):  
Hideki Kanokogi ◽  
Saiho Ko ◽  
Hiromichi Kanehiro ◽  
Michiyoshi Hisanaga ◽  
Yukihiro Tatekawa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Small Bowel ◽  
Immune Responses ◽  
Mesenteric Lymph Node ◽  
Small Bowel Transplantation ◽  
Mesenteric Lymph

Transfer of immunity toTrichinella spiralisin the mouse with mesenteric lymph node cells: time of appearance of effective cells in donors and expression of immunity in recipients

Parasitology ◽  
1977 ◽  
Vol 74 (3) ◽  
pp. 215-224 ◽  
Author(s):  
D. Wakelin ◽  
Margaret M. Wilson
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Time Course ◽  
Trichinella Spiralis ◽  
Defence Mechanism ◽  
Spleen Cells ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Worm Expulsion

Cells capable of transferring immunity toTrichinella spiralis, i.e. of accelerating adult worm expulsion, were present in the mesenteric lymph nodes of mice infected for 4, 6 or 8 days, but not in mice infected for only 2 days. The time-course of worm expulsion in mice infected on the day of transfer was similar in recipients of day 8 cells, expulsion becoming marked only when the recipients had been infected for at least 6 days. Transfer of cells 4 or 6 days after infection did not result in an accelerated worm expulsion; transfer 1 or 2 weeks before infection did not enhance the level of immunity in recipient mice. In contrast to the results obtained with mesenteric lymph node cells (MLNC) no immunity was trsnsferred when recipients were given spleen cells taken from donors infected for 8 days. It is suggested that MLNC do not cause worm expulsion directly, but cooperate with another component of the host's defence mechanism. Accelerated expulsion in recipients of cells was accompanied by a premature decline in fecundity of female worms. Evidence is presented to show that worm expulsion and impaired reproduction may represent independent aspects of the immune response toT. spiralis.

scholarly journals EFFECT OF RECENT ANTIGEN PRIMING ON ADOPTIVE IMMUNE RESPONSES

1973 ◽  
Vol 138 (1) ◽  
pp. 143-162 ◽  
Author(s):  
J. Sprent ◽  
J. F. A. P. Miller
Keyword(s):  
Immune Responses ◽  
B Lymphocytes ◽  
Transient State ◽  
Antibody Responses ◽  
Partial Recovery ◽  
Cell Transfer ◽  
Spleen Cells ◽  
Experimental Conditions ◽  
Mesenteric Lymph ◽  
Normal Spleen

When spleen, mesenteric lymph node, or Peyer's patch cells from mice primed 24 h before with either sheep erythrocytes (SRC) or horse erythrocytes (HRC) were transferred together with both SRC and HRC to irradiated mice, antibody responses measured 7 days later were very low to the priming antigen but high to the other antigen. This was demonstrated either by measuring numbers of antibody-forming cells in spleen or levels of hemagglutinins in serum. Specific unresponsiveness of the transferred cells was evident in both the 19S and 7S responses. It was observed only when strict experimental conditions were followed: (a) the cell donors had to be primed with not less than 109 erythrocytes given intravenously; (b) the cells had to be transferred between 1 and 2 days after antigen priming; (c) antibody responses in the recipients were measured within 7 days of cell transfer, i.e., partial recovery was evident by 11 days; (d) the transferred cells had to be challenged in the recipients within 1 day after cell transfer: when challenge was delayed for 5 days or longer, responsiveness returned. The failure of cells from recently primed donors to respond to the priming antigen on adoptive transfer could be overcome by supplementing with normal spleen cells, but not with thymus alone or bone marrow alone. This implied that unresponsiveness occurred at the levels of both T and B lymphocytes, and was not due to a suppressive influence exerted by T cells. Further work is in progress to determine the mechanism of this transient state of specific unresponsiveness.

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