Mesenteric lymph node stroma cells in the generation of intestinal immune responses

ABSTRACT Recombinant antigens of Cryptosporidium parvum, Cp900 and Cp40 but not Cp15, stimulated C. parvum-specific proliferative immune responses of mesenteric lymph node cells in C57BL/6J mice infected with different isolates (MD, GCH1, UCP, and IOWA) of C. parvum, indicating that both Cp900 and Cp40 are immunodominant targets of cellular immune responses during C. parvum infection.

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Leptin and adiponectin supplementation modifies mesenteric lymph node lymphocyte composition and functionality in suckling rats

British Journal Of Nutrition ◽

10.1017/s0007114517003786 ◽

2018 ◽

Vol 119 (5) ◽

pp. 486-495 ◽

Cited By ~ 10

Author(s):

Blanca Grases-Pintó ◽

Mar Abril-Gil ◽

Maria J. Rodríguez-Lagunas ◽

Margarida Castell ◽

Francisco J. Pérez-Cano ◽

...

Keyword(s):

Lymph Node ◽

Immune Responses ◽

Mesenteric Lymph Node ◽

Early Life ◽

Suckling Period ◽

Mesenteric Lymph Nodes ◽

Trophic Effect ◽

Mesenteric Lymph ◽

Intestinal Immune System ◽

Intestinal Iga

AbstractAt birth, when immune responses are insufficient, there begins the development of the defence capability against pathogens. Leptin and adiponectin, adipokines that are present in breast milk, have been shown to play a role in the regulation of immune responses. We report here, for the first time, the influence of in vivo adipokine supplementation on the intestinal immune system in early life. Suckling Wistar rats were daily supplemented with leptin (0·7 μg/kg per d, n 36) or adiponectin (35 μg/kg per d, n 36) during the suckling period. The lymphocyte composition, proliferation and cytokine secretion from mesenteric lymph node lymphocytes (on days 14 and 21), as well as intestinal IgA and IgM concentration (day 21), were evaluated. At day 14, leptin supplementation significantly increased the TCRαβ+ cell proportion in mesenteric lymph nodes, in particular owing to an increase in the TCRαβ+ CD8+ cell population. Moreover, the leptin or adiponectin supplementation promoted the early development CD8+ cells, with adiponectin being the only adipokine capable of enhancing the lymphoproliferative ability at the end of the suckling period. Although leptin decreased intestinal IgA concentration, it had a trophic effect on the intestine in early life. Supplementation of both adipokines modulated the cytokine profile during (day 14) and at the end (day 21) of the suckling period. These results suggest that leptin and adiponectin during suckling play a role in the development of mucosal immunity in early life.

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EFFECT OF RECENT ANTIGEN PRIMING ON ADOPTIVE IMMUNE RESPONSES

Journal of Experimental Medicine ◽

10.1084/jem.139.1.1 ◽

1974 ◽

Vol 139 (1) ◽

pp. 1-12 ◽

Cited By ~ 31

Author(s):

J. Sprent ◽

J. F. A. P. Miller

Keyword(s):

Lymph Node ◽

Immune Responses ◽

Mesenteric Lymph Node ◽

Antibody Responses ◽

The Other ◽

Spleen Cells ◽

Antigen Concentration ◽

Short Term ◽

Mesenteric Lymph ◽

Circulating Lymphocytes

When thoracic duct lymphocytes (TDL) or mesenteric lymph node (MLN) cells from mice primed 1 day before with either sheep erythrocytes (SRC) or horse erythrocytes (HRC) were transferred together with both SRC and HRC to irradiated mice, antibody responses measured 7 days later could not be detected to the priming antigen but were high to the other antigen. Furthermore, this unresponsiveness of TDL and MLN to the priming antigen could not be abrogated by delaying antigen challenge of the transferred cells for 1–2 wk. Previous work had shown that short-term priming with antigen also induced specific unresponsiveness in spleen cells on adoptive transfer. Unresponsiveness in these cells, however, was only of temporary duration, full recovery in the reactivity of the cells being observed when challenge with the priming antigen on transfer was delayed for 5 or more days. Since the present work showed that such recovery from initial unresponsiveness on transfer was unique to spleen cells and did not apply to TDL or MLN, it appeared that different mechanisms were responsible for the unresponsiveness in the three populations. It is proposed that the unresponsiveness detected in TDL and MLN cells in the present study resulted from a deficiency of antigen-reactive cells, these cells having been recruited to the spleen, i.e., a region of antigen concentration. This concept of antigen-induced selective recruitment of circulating lymphocytes was supported by evidence that 51Cr-labeled heterologous erythrocytes indeed localized largely in the spleen after intravenous injection but not in MLN.

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CD markers of camel (Camelusdromedarius) intestine naturally infected with Mycobacteriumavium subsp. paratuberculosis: Distinct expression of Madcam-1 and CX3CR1

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES ◽

10.3906/vet-2003-5 ◽

2020 ◽

Vol 44 (5) ◽

pp. 1010-1023

Author(s):

Saeed AL-RAMADAN ◽

Kazem AL-MOHAMMED SALEM ◽

Ibrahim ALSHUBAITH ◽

Ahmed ALLUWAIMI

Keyword(s):

Lymph Node ◽

Immune Responses ◽

Lamina Propria ◽

Mesenteric Lymph Node ◽

Immunohistochemical Staining ◽

Vital Role ◽

High Expression ◽

Macrophage Phenotype ◽

Mesenteric Lymph ◽

Cd Markers

Mycobacteriumavium subsp. paratuberculosis (MAP) infection in camel requires extensive research, particularly the immune responses in the intestine. This study aimed to investigate the nature of the cellular populations that are driven by the immunopathological responses in the camel intestine infected with MAP at different ages. Immunohistochemical staining was carried out on tissues obtained from naturally infected young (5–10 years old) and older (12–15 years old) camels. The staining of the tissues, ileum, mesenteric lymph node, jejunum, and supramammary lymph nodes, with anti-CD3+, CD4+, CD8+, CD25+, CD11c+, CD14+, WC1+, CX3CR1, and Madcam-1 monoclonal antibodies revealed high expression of the molecules CD8+, CD25+, CD11c+, CD14+, WC1+, CX3CR1, and Madcam-1 in the ileum and mesenteric lymph node of the infected older camels. The results indicated the recruitment of CD8+lymphocytes, CD14+ macrophages, and CD11c+ dendritic cells to the ileal lamina propria. High expression of CX3CR1 could indicate a vital role for this special macrophage phenotype in the ileal lamina propria in maintaining intestinal homeostasis. Madcam-1 expression could have an essential role in defining the nature of the recruited cells to the site of the infection. Expression of CX3CR1 and Madcam-1 is a novel finding that merits further attention and pursuit to reveal their significance in the immune responses to MAP in the camel’s intestine.

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Immune responses of graft mesenteric lymph node in small bowel transplantation

Journal of Surgical Research ◽

10.1016/j.jss.2003.10.004 ◽

2004 ◽

Vol 116 (2) ◽

pp. 269-276 ◽

Cited By ~ 4

Author(s):

Hideki Kanokogi ◽

Saiho Ko ◽

Hiromichi Kanehiro ◽

Michiyoshi Hisanaga ◽

Yukihiro Tatekawa ◽

...

Keyword(s):

Lymph Node ◽

Small Bowel ◽

Immune Responses ◽

Mesenteric Lymph Node ◽

Small Bowel Transplantation ◽

Mesenteric Lymph

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Neutralizing interleukin 18 (IL-18) differentially regulates immune responses from the mesenteric lymph node compared to the lamina propria in Il-10-deficient mice

Gastroenterology ◽

10.1016/s0016-5085(01)83444-2 ◽

2001 ◽

Vol 120 (5) ◽

pp. A692-A692

Author(s):

D SPENCER ◽

R JUMP ◽

A LEVINE

Keyword(s):

Lymph Node ◽

Immune Responses ◽

Lamina Propria ◽

Mesenteric Lymph Node ◽

Interleukin 18 ◽

Mesenteric Lymph ◽

Deficient Mice

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TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of Schistosoma japonicum-Infected C57BL/6 Mice

Journal of Immunology Research ◽

10.1155/2019/2691808 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14

Author(s):

Jiale Qu ◽

Xiuxue Yu ◽

Chenxi Jin ◽

Yuanfa Feng ◽

Shihao Xie ◽

...

Keyword(s):

T Cells ◽

Lymph Node ◽

T Cell ◽

Immune Responses ◽

Mesenteric Lymph Node ◽

Cell Response ◽

Early Phase ◽

T Cell Response ◽

Mesenteric Lymph ◽

Tlr7 Agonist

Toll-like receptors (TLRs) play an important role in regulating immune responses during pathogen infection. However, roles of TLRs on T cells reside in the mesenteric lymph node (MLN) were not be fully elucidated in the course of S. japonicum infection. In this study, T lymphocytes from the mesenteric lymph node (MLN) of S. japonicum-infected mice were isolated and the expression and roles of TLR2, TLR3, TLR4, and TLR7 on both CD4+ and CD8+ T cells were compared. We found that the expression of TLR7 was increased in the MLN cells of S. japonicum-infected mice, particularly in CD4+ and CD8+ T cells (P<0.05). R848, a TLR7 agonist, could enhance the production of IFN-γ from MLN T cells of infected mice (P<0.05), especially in CD8+ T cells (P<0.01). In TLR7 gene knockedout (KO) mice, the S. japonicum infection caused a significant decrease (P<0.05) of the expression of CD25 and CD69, as well as the production of IFN-γ and IL-4 inducted by PMA plus ionomycin on both CD4+ and CD8+ T cells. Furthermore, the decreased level of IFN-γ and IL-4 in the supernatants of SEA- or SWA-stimulated mesenteric lymphocytes was detected (P<0.05). Our results indicated that S. japonicum infection could induce the TLR7 expression on T cells in the MLN of C57BL/6 mice, and TLR7 mediates T cell response in the early phase of infection.

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