Cone beam geometry for small objects in phase contrast tomography

2013 ◽  
Vol 29 (9) ◽  
pp. 095013 ◽  
Author(s):  
P Jonas ◽  
A K Louis
Keyword(s):  
Phase Contrast ◽  
Cone Beam ◽  
Beam Geometry ◽  
Phase Contrast Tomography

scholarly journals Polychromatic cone-beam phase-contrast tomography

2007 ◽  
Vol 76 (4) ◽  
Author(s):  
G. R. Myers ◽  
S. C. Mayo ◽  
T. E. Gureyev ◽  
D. M. Paganin ◽  
S. W. Wilkins
Keyword(s):  
Phase Contrast ◽  
Cone Beam ◽  
Phase Contrast Tomography

First laboratory X-ray diffraction contrast tomography for grain mapping of polycrystals

2013 ◽  
Vol 46 (6) ◽  
pp. 1734-1740 ◽  
Author(s):  
A. King ◽  
P. Reischig ◽  
J. Adrien ◽  
W. Ludwig
Keyword(s):  
Phase Contrast ◽  
Radiation Spectrum ◽  
Three Dimensional ◽  
Cone Beam ◽  
X Ray Diffraction ◽  
X Ray ◽  
Beam Geometry ◽  
First Results ◽  
Further Development ◽  
Polychromatic Radiation

The first results of three-dimensional grain mapping using a laboratory tomograph equipped with a microfocus W target X-ray tube source, operated at 90 kV and 350 µA, are presented. Adapted algorithms exploit the polychromatic radiation spectrum and the projection magnification arising from the cone-beam geometry. The first map of grain shapes and crystallographic orientations from a titanium sample containing 42 grains is presented and its validity confirmed by a phase contrast reconstruction of the grain boundaries. Perspectives are given for the further development of the technique to accommodate samples with more grains or with greater intragranular orientation spread.

scholarly journals Forward model for propagation-based x-ray phase contrast imaging in parallel- and cone-beam geometry

2019 ◽  
Vol 27 (4) ◽  
pp. 4504 ◽  
Author(s):  
Elisabeth R. Shanblatt ◽  
Yongjin Sung ◽  
Rajiv Gupta ◽  
Brandon J. Nelson ◽  
Shuai Leng ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Forward Model ◽  
Cone Beam ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Beam Geometry

scholarly journals 3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Marina Eckermann ◽  
Jasper Frohn ◽  
Marius Reichardt ◽  
Markus Osterhoff ◽  
Michael Sprung ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Phase Contrast ◽  
3D Visualization ◽  
Diffuse Alveolar Damage ◽  
Three Dimensional ◽  
Cone Beam ◽  
X Ray ◽  
Multi Scale ◽  
Beam Geometry ◽  
Third Dimension

We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment.

scholarly journals 3d Virtual Pathohistology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography

2020 ◽  
Author(s):  
M. Eckermann ◽  
J. Frohn ◽  
M. Reichardt ◽  
M. Osterhoff ◽  
M. Sprung ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Phase Contrast ◽  
Diffuse Alveolar Damage ◽  
Three Dimensional ◽  
Cone Beam ◽  
X Ray ◽  
Multi Scale ◽  
Beam Geometry ◽  
Conventional Histology ◽  
Third Dimension

We present a new approach of three-dimensional (3d) virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a novel tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 4 mm are scanned and reconstructed at a resolution and image quality which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3d visualisation of the diffuse alveolar damage with its prominent hyaline membrane formation, by mapping the 3d distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment.

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