Using of RAPD-PCR markers to detect the genetic relationship of number of Bellevalia Lapeyr and Ornithogalum L. species developing in central and northern of Iraq

This study aims to determine the genetic relationship between four strains of guppy, albino full platinum (AFP), albino german yellow (AGY), top sword (TS) and guppy yellow cobra (GYC) using the RAPD-PCR method. This study used explorative method without experimental design and analyzed by descriptive qualitative and quantitative. The obtained genetic relationship data could be used as data reference for hybridization between strains of guppy fish that have been researched. The research was conducted in October 2020-April 2021. The three fish samples (AFP, TS and GYC) obtained from fish breeder in Cilengkrang-Bandung and AGY sample obtained from fish breeder in Tanggerang-Banten. Based on the results of amplification using OPA-03 primer (AGTCAGCCAC), four strains of guppy fish showed 30 DNA bands that included polymorphic and monomorphic bands. The AFP strains had 19 monomorphic bands, AGY had 21 DNA bands (20 monomorphic bands and one polymorphic bands), TS had 19 DNA bands (17 monomorphic bands and two polymorphic bands) and GYC had 15 DNA bands (14 monomorphic bands and one polymorphic band). Phylogenetic tree analyzed by NTSys program. It is shown between AFP and AGY strains had 95% relationship index, then between TS and GYC strains had 82% relationship index and between AFP-AGY and TS-GYC had 50% relationship index.

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Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v4i430111 ◽

2020 ◽

pp. 1-9

Author(s):

Ayi Yustiati ◽

Reifolnanda Mangappu Hutagalung ◽

Yuli Andriani ◽

Walim Lili ◽

Ibnu Bangkit

Keyword(s):

Genetic Relationship ◽

Oreochromis Niloticus ◽

Dna Isolation ◽

Random Amplified Polymorphic Dna ◽

Geographical Isolation ◽

Genetic Stock ◽

Internal Factors ◽

Rapd Pcr ◽

Aceh Province ◽

Relationship Of

This research was carried out from June to November 2017. This research aims to analyze genetic relationship of tilapia (Oreochromis niloticus) nirwana strain which is cultured in Aceh Besar (Aceh Province) and in Wanayasa (West Java Province) with RAPD-PCR technique. The DNA Isolation Activity of tilapia nirwana strain was carried out in The Biotechnology Laboratory, Building No.4, FPIK UNPAD, Indonesia and the PCR process was carried out in The Microbiology and Biotechnology Laboratory, Building No.3 FPIK UNPAD, Indonesia. The purpose of this research is to determine the level of genetic relationship of tilapia nirwana strain cultured in Aceh Besar and Wanayasa and to find out whether tilapia nirwana strain in Aceh Besar can be used as a genetic stock. The results showed that genetic relationship level of tilapia nirwana strain cultured in Aceh Besar with those cultured in Wanayasa is 67%. The existence of environmental differences results in genetic diversity between Aceh Besar and Wanayasa tilapia nirwana strain. Factors that causing grouping movement are external factors such as: Geographical isolation and habitat fragmentation as well as internal factors such as mutation, natural selection, genetic drift, and gene flow. Tilapia nirwana strain that is cultured in Aceh Besar produce polymorphic bands, so it can be used as a new genetic stock to stabilize the tilapia nirwana strain production.

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Genetic Distance of Four Strains of Guppy Fish (Poecilia reticulata) Using RAPD PCR Method

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v10i130228 ◽

2021 ◽

pp. 1-8

Author(s):

Ayi Yustiati ◽

Aisyah Nuryanti ◽

Ibnu Bangkit Bioshina Suryadi ◽

Yuniar Mulyani

Keyword(s):

Genetic Relationship ◽

Poecilia Reticulata ◽

Similarity Index ◽

Central Laboratory ◽

Rapd Pcr ◽

Standard Parameter ◽

Pcr Method ◽

The Tropics ◽

Relationship Of ◽

Guppy Fish

Guppies are one type of organism that can live well, especially in the tropics. The kinship between species that have genetic similarities can be identified through genotypic mapping, one of which is molecular analysis using. RAPD method with PCR technique. This study aims to determine the genetic relationship of four guppy poecilia reticulata strains, respectively are Albino Full Red (AFP), Brazilian Fan Tail (BFT), Koi Guppy Tuxedo (KGT), and Platinum Red Tail Big Ears (PRTB) with the RAPD-PCR method. The genetic relationship data obtained is used as a guide for mating between the four strains. This research was conducted from September 2020 to November 2020. The process were carried out at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University and the Central Laboratory, Padjadjaran University. OPA-03 primer (AGTCAGCCAC) is used as a standard parameter to interpret genetic diversity among the four guppy strains. Based on the results, amplification with primer OPA-03 visualized 21 bands consisting of seven polymorphic bands and 13 monomorphic bands. The results of the phylogenetic tree showed that there were two groups. The first group is AFR and BFT a similarity index of 69.5%. The second group is KGT and PRTB a similarity index of 71.5%.

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Genetic Relationship of Ramie Germplasm Revealed by RSAP, SRAP, and SSR Markers

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2012.00840 ◽

2013 ◽

Vol 38 (5) ◽

pp. 840-847 ◽

Cited By ~ 5

Author(s):

Zi-Zheng ZOU ◽

Jian-Hua CHEN ◽

Ming-Bao LUAN ◽

Jin-Xia GUO ◽

Chao WANG ◽

...

Keyword(s):

Ssr Markers ◽

Genetic Relationship ◽

Relationship Of

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Dante's conception of the genetic relationship of European languages

Neophilologus ◽

10.1007/bf01513985 ◽

1979 ◽

Vol 63 (2) ◽

pp. 173-178

Author(s):

P. Robert Paustian

Keyword(s):

Genetic Relationship ◽

European Languages ◽

Relationship Of

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Assessment of Genetic Relationship of Foxtail Millet with Its Wild Ancestor and Close Relatives by ISSR Markers

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(12)60042-2 ◽

2012 ◽

Vol 11 (4) ◽

pp. 556-566 ◽

Cited By ~ 9

Author(s):

Wei LI ◽

Hui ZHI ◽

Yong-fang WANG ◽

Hai-quan LI ◽

Xian-mm DIAO

Keyword(s):

Genetic Relationship ◽

Foxtail Millet ◽

Issr Markers ◽

Wild Ancestor ◽

Close Relatives ◽

Relationship Of

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Genetic relationship of parents and progeny derived from crossed pollination of Piper nigrum L. based on morphology and RAPD markers

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/762/1/012070 ◽

2021 ◽

Vol 762 (1) ◽

pp. 012070

Author(s):

N L W Meilawati ◽

J Darajat ◽

N Bermawie

Keyword(s):

Genetic Relationship ◽

Rapd Markers ◽

Piper Nigrum ◽

Relationship Of

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Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Polish Journal of Veterinary Sciences ◽

10.2478/v10181-011-0044-5 ◽

2011 ◽

Vol 14 (2) ◽

pp. 285-286 ◽

Cited By ~ 6

Author(s):

J. Karakulska ◽

A. Pobucewicz ◽

P. Nawrotek ◽

M. Muszyńska ◽

A. Furowicz ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Species Identification ◽

Genetic Relationship ◽

Molecular Typing ◽

Rapd Analysis ◽

Milk Samples ◽

Rapd Pcr ◽

Pcr Rflp ◽

Mastitic Milk ◽

Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

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