Genetic Distance of Four Strains of Guppy Fish (Poecilia reticulata) Using RAPD PCR Method

Guppies are one type of organism that can live well, especially in the tropics. The kinship between species that have genetic similarities can be identified through genotypic mapping, one of which is molecular analysis using. RAPD method with PCR technique. This study aims to determine the genetic relationship of four guppy poecilia reticulata strains, respectively are Albino Full Red (AFP), Brazilian Fan Tail (BFT), Koi Guppy Tuxedo (KGT), and Platinum Red Tail Big Ears (PRTB) with the RAPD-PCR method. The genetic relationship data obtained is used as a guide for mating between the four strains. This research was conducted from September 2020 to November 2020. The process were carried out at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University and the Central Laboratory, Padjadjaran University. OPA-03 primer (AGTCAGCCAC) is used as a standard parameter to interpret genetic diversity among the four guppy strains. Based on the results, amplification with primer OPA-03 visualized 21 bands consisting of seven polymorphic bands and 13 monomorphic bands. The results of the phylogenetic tree showed that there were two groups. The first group is AFR and BFT a similarity index of 69.5%. The second group is KGT and PRTB a similarity index of 71.5%.

Does anthropogenic introduction of guppy fish (Poecilia reticulata) impact faunal species diversity and abundance in natural aquatic habitats? A systematic review protocol

Background The guppy fish (Poecilia reticulata) is a tropical fish ancestrally linked to northern South America and the Caribbean. It is known to be very tolerant of and adaptable to new environments, and able to consume multiple food sources, including mosquito larvae. Consequently, guppies have been frequently introduced to non-native ecosystems to control mosquito populations, resulting in near-global distribution. Indeed, due to the increasing need for mosquito-borne disease control, guppy releases will likely continue, however there are concerns about potential adverse impacts on other species, biodiversity, and certain ecosystem functions. The most significant of these is local species extinction, and by extension, reduced biodiversity. Yet, the extent of these impacts has not been evaluated by scientific review. Accordingly, this study will examine and evaluate whether anthropogenic introduction of guppy fish (Poecilia reticulata) has impacts on faunal species diversity and abundance in natural aquatic habitats. The results of this review may have implications for environmental management and policy and inform ecosystem-based integrated vector management and public health policy. Methods Relevant scientific articles will be identified by searching electronic databases. Articles will be included if they report changes or differences, associated with guppy fish introduction, in at least one of these population parameters: (1) abundance of individuals in any species, (2) total abundance of individuals in all species present, (3) species richness, (4) species diversity, and (5) community evenness. Each article will be assessed by at least two independent reviewers against pre-defined inclusion/exclusion criteria. Snowballing reference lists of included articles will be conducted. At least two reviewers will critically appraise all included studies using the Collaboration for Environmental Evidence Critical Appraisal Tool (CEECAT) and any discrepancies will be resolved by discussion between the two or adjudication by a third author if agreement is not reached. Each study will also be subjected to data extraction against pre-defined qualitative and quantitative outcomes and results will be tabulated/presented in figures where appropriate. A meta-analysis will be carried out on outcome parameters with sufficient evidence.

The Acute Single and Mixture Toxicity of Paraquat Dichloride and Deltamethrin to Guppy (Poecilia reticulata)

Although herbicide and insecticide contamination of surface waters normally occurs in the form of mixtures, the toxicity interactions displayed by such mixtures have only rarely been characterized. This study evaluated the acute effects of single pesticides (paraquat dichloride and deltamethrin, tested in their commercial formulations Gramoxone 276 SL and Decis 25EC) and their equitoxic mixtures on the survival of adult male guppy fish (Poecilia reticulata). Mixture toxicity was evaluated against the reference models of Concentration Addition (CA) and Independent Action (IA). In the single treatments, the 72h LC10 and LC50 values were 1.5 and 6.0 mg a.s. L-1 and 0.53 and 3.6 µg a.s. L-1 for paraquat dichloride and deltamethrin, respectively. The equitoxic mixtures showed significant paraquat dichloride-deltamethrin antagonism, both based on the CA and the IA model, without significant dose-level dependent deviations.

Mucosal Immune Responses and Total Carotenoid Content of Juvenile Male Guppy Fish (Poecilia Reticulata) Fed With Different Herbal Supplements ​

In this experiment, the influences of dietary garlic, onion, and sweet red pepper meals as herbal supplements were studied on some immunological parameters, total carotenoid content, and growth performance of male guppy fish (Poecilia reticulata) in the juvenile stage. The experimental diets were fed to fish as a control (0), and three other diets contained 20 g kg− 1 garlic, or 20 g kg− 1 red onion, and or 20 g kg− 1 sweet red pepper powders. The male guppies with the early weight of 0.642g ± 0.07 were stocked in twelve circular tanks (40 L capacity) at density of 30 fish per tank. Fish were fed to satiation the diets thrice daily. The results showed that dietary herbal additives induced a significant increase (P < 0.05) in some mucosal immunological parameters contained alternative complement (ACH50), total immunoglobulin (Ig), lysozyme, and total protein compared to control, which were the highest in the group with 20 g kg− 1 garlic powder. Also, various dietary herbal additives resulted in higher (P < 0.05) total carotenoids levels of lateral skin body and caudal fin of fish than control, which were the highest in the group with 20 g kg− 1 sweet red pepper. The growth performance and feeding indices enhanced significantly (P < 0.05) in experimental groups compared to the control. As a result, used herbal supplements had beneficial effects on immunological parameters and total carotenoid content of fish. So, the highest immunity and coloration was obtained separately at 20 g kg− 1 respectively with garlic and sweet red pepper meals.

Using Inter Simple Sequence Repeat Multi-Loci Markers for Studying Genetic Diversity in Guppy Fish

Aquarium fish industry has become a capacity for job creation and income generation. Aquarium fish uses for decoration and creating tranquility in the environment and beautification in public places. This study was conducted to investigate genetic variation between different populations of various varieties of guppy ornamental aquarium fish using inter simple sequence repeat (ISSR) markers in Iran for the first time. DNA was extracted from twelve strains. Polymerase chain reaction was performed using seven ISSR primers. GenAlex 6.501 and XLSTAT software was used to analyze data. Results of analysis of molecular variance showed that 54% of observed polymorphism belongs to variance among populations and 46% corresponds to diversity between populations. The highest and the lowest polymorphism percentage belonged to (GAG)5GC (90%) and (AG)8C (41%) markers, respectively. Percentage of polymorphic loci ranges from 32.31% to 86.15%. The results of phylogenetic tree showed that this tree was divided into 2 major clusters. Cluster I included only 2 populations and cluster II contained other 10 studied populations. It is suggested that this marker system was found to be efficient in discriminating each genotype at the molecular level and can be used for genetic diversity analysis for aquarium guppy fish because a greater understanding of the potential of the species is necessary for supporting long-term genetic improvement.

Aquarium Water Quality Monitoring Based On Internet Of Things

Guppy fish is one type of ornamental fish that is widely kept in aquariums. Water temperature, water pH, and water turbidity are important factors for the life and development of guppy fish in an aquarium. The ideal water conditions for guppy fish in the aquarium are 23-27oC, a pH value of 6.5-7.5, and a water turbidity level of 0-25 NTU. To maintain ideal water conditions, research is needed to keep the aquarium water conditions stable. The research was conducted by experimenting using the NodeMCU platform as a microcontroller and the DS18B20 temperature sensor for sensing water temperature, a pH sensor 4502C, and a TDS sensor for sensing water turbidity. Testing is done by detecting the condition of the aquarium water with all sensors simultaneously to find out the water conditions in real time. The test results show that the tool designed is able to maintain water temperature at 23-27 oC, maintain pH values at 6.5-7.5, and water turbidity at 0-25 NTU. Thus the tool is able to maintain the ideal aquarium water quality for guppies.

Effect of Different Dietary Protein Levels on Survival Rate and Growth Per-formance of Guppy (Poecilia reticulata)

This study was carried out to evaluate the growth response of guppy Poecilia reticulata to different dietary protein levels. A total of 72 fry of guppy fish of an initial body weight 0.365± 0.01g were distributed randomly into 60 L aquaria at the density rate of 0.05 g/L. Fish were fed to satiation twice daily for eight weeks with diets containing different dietary protein levels (20%, 30%, or 40%.). The survival rate of guppy was unaffected by increasing dietary protein levels. At the end of the experiment, weight gains (g) ranging from 1.93 to 3.55 were obtained corresponding to 20% and 40% crude protein respectively. The growth performance and feeding efficiencies were significantly improved (P < 0.05) by increasing dietary protein levels. However, no significant difference (P > 0.05) was observed in the specific growth rate of fish that were fed 20% and 30% dietary protein levels. The value of the condition factor was significantly high in fish fed at 40% dietary protein. It can be concluded that a 40% dietary protein level is recommended for guppy fish.

The Acute Single and Mixture Toxicity of Paraquat Dichloride and Deltamethrin to Guppy (Poecillia reticulata)

Although herbicide and insecticide contamination of surface waters normally occurs in the form of mixtures, the toxicity interactions displayed by such mixtures have only rarely been characterized. This study evaluated the acute effects of single pesticides (paraquat dichloride and deltamethrin, tested in their commercial formulations Gramoxone 276 SL and Decis 25EC) and their equitoxic mixtures on the survival of adult male guppy fish (Poecilia reticulata). Mixture toxicity was evaluated against the reference models of Concentration Addition (CA) and Independent Action (IA). In the single treatments, the 72h LC10 and LC50 values were 1.5 and 6.0 mg a.s. L-1 and 0.53 and 3.6 µg a.s. L-1 for paraquat dichloride and deltamethrin, respectively. The equitoxic mixtures showed significant paraquat dichloride-deltamethrin antagonism, both based on the CA and the IA model, without significant dose-level dependent deviations.

Effect of background color on the growth pattern and coloration of guppy (Poecilia reticulata)

A study was carried out to investigate the effects of background color on the growth, skin pigmentation and breeding performances of guppy, Poecilia reticulata. Guppy fish was reared in tanks with four different colors as treatments such as T0 (transparent), T1 (red), T3 (blue) and T4 (green) for 5 months. There were significant differences in growth and coloration among the treatments. The growth performance was found significantly higher in transparent background compared to the other treatments. The results showed that the highest mean weight (0.259 ±0.018 g), mean length (3.067±0.208 cm) and SGR (0.165±0.012) of the fish were significantly higher in T0. Least mean weight (0.167± 0.025 g), mean length (2.53±.0.058 cm) and SGR (0.104±0.017) were observed in T1 indicating association of red color with slower growth performance of guppy, compared to other treatments. Study showed significant influence of background color in skin pigmentation. Fishes reared in transparent tanks showed the highest value of carotenoid (0.00175±0.00004mg/g) and fishes reared in the red tank showed the lowest value of carotenoid (0.00092±0.000035 mg/g). Green background enhanced red orange color on fish skin and provided high amount of carotenoid value (0.00143±0.000046 mg/g) than that with blue (0.00095±0.000025 mg/g). These results suggest significant influence of background color on growth and skin pigmentation patterns of Guppy.

Avoidance Response: A More Important Determinant of Population Immediate Decline in Guppy Fish (Poecilia Reticulata Peters, 1859) Exposed to Pesticides.

This study, investigated the ability of Paraquat (herbicide) and Dichlorvos (insecticide) to elicit avoidance response in population of Guppy fish, Poecilia reticulata and predict the population immediate decline (PID) of P. reticulata when exposed to both agro-chemicals. A 96-h forced system (FS) bioassay was each conducted in five duplicate systems, each with a control experiment. The avoidance response was examined using non-forced multi-compartmented static system (NFS). The guppies (n = 3 guppies per concentration of 6 compartments in quadruplet) were exposed to a gradient of Paraquat (0, 2.5, 5.0, 7.5, 10.0 and 15.0 mg/L) and Dichlorvos (0, 1.6, 2.6, 3.0, 3.2 and 4.0 mg/L). Their distributions were examined at 20 min intervals for a 3-h period (n = 9 observation periods). The results from 96-h FS were does-dependent with highest percentage mortality being 85.7% for Paraquat and 78.6% for Dichlorvos in respective highest concentrations. The 3-h NFS exposure showed statistically significant concentration-dependent spatial avoidance. The P. reticulata avoided the lowest concentration of Paraquat (2.5mg/L) and Dichlorvos (1.6 mg/L) by 68.3% and 48.3% respectively. The avoidance increased significantly (p < 0.005) to 75%, for the highest concentrations of both test chemicals (15.0 and 4.0 mg/L). The 3-hr AC50 value revealed that the guppies were more sensitive to Paraquat (0.37 mg/L) than Dichlorvos (1.73 mg/L). The PID was driven by the avoidance behaviour (NFS) of the guppies rather than mortality (FS). The FS heretofore overestimate environmental risk assessment of contamination due to mandatory exposure of mobile organisms to contaminants. Rather than the traditional FS systems, NFS should be adopted and incorporated in typical bioassays for better predictive results.