Genetic Distance of Four Strains of Guppy Fish (Poecilia reticulata) Using RAPD PCR Method

Guppies are one type of organism that can live well, especially in the tropics. The kinship between species that have genetic similarities can be identified through genotypic mapping, one of which is molecular analysis using. RAPD method with PCR technique. This study aims to determine the genetic relationship of four guppy poecilia reticulata strains, respectively are Albino Full Red (AFP), Brazilian Fan Tail (BFT), Koi Guppy Tuxedo (KGT), and Platinum Red Tail Big Ears (PRTB) with the RAPD-PCR method. The genetic relationship data obtained is used as a guide for mating between the four strains. This research was conducted from September 2020 to November 2020. The process were carried out at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University and the Central Laboratory, Padjadjaran University. OPA-03 primer (AGTCAGCCAC) is used as a standard parameter to interpret genetic diversity among the four guppy strains. Based on the results, amplification with primer OPA-03 visualized 21 bands consisting of seven polymorphic bands and 13 monomorphic bands. The results of the phylogenetic tree showed that there were two groups. The first group is AFR and BFT a similarity index of 69.5%. The second group is KGT and PRTB a similarity index of 71.5%.

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Genetic Relationship of Four Strains of Guppy (Poeciliareticulata Peters, 1859) Using RAPD-PCR Method

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v7i430180 ◽

2021 ◽

pp. 15-24

Author(s):

Ine Triana Nuradha ◽

Ayi Yustiati ◽

Asep Agus Handaka ◽

Ibnu Bangkit

Keyword(s):

Experimental Design ◽

Genetic Relationship ◽

Polymorphic Band ◽

Qualitative And Quantitative ◽

Rapd Pcr ◽

Fish Samples ◽

Pcr Method ◽

Polymorphic Bands ◽

Relationship Of ◽

Guppy Fish

This study aims to determine the genetic relationship between four strains of guppy, albino full platinum (AFP), albino german yellow (AGY), top sword (TS) and guppy yellow cobra (GYC) using the RAPD-PCR method. This study used explorative method without experimental design and analyzed by descriptive qualitative and quantitative. The obtained genetic relationship data could be used as data reference for hybridization between strains of guppy fish that have been researched. The research was conducted in October 2020-April 2021. The three fish samples (AFP, TS and GYC) obtained from fish breeder in Cilengkrang-Bandung and AGY sample obtained from fish breeder in Tanggerang-Banten. Based on the results of amplification using OPA-03 primer (AGTCAGCCAC), four strains of guppy fish showed 30 DNA bands that included polymorphic and monomorphic bands. The AFP strains had 19 monomorphic bands, AGY had 21 DNA bands (20 monomorphic bands and one polymorphic bands), TS had 19 DNA bands (17 monomorphic bands and two polymorphic bands) and GYC had 15 DNA bands (14 monomorphic bands and one polymorphic band). Phylogenetic tree analyzed by NTSys program. It is shown between AFP and AGY strains had 95% relationship index, then between TS and GYC strains had 82% relationship index and between AFP-AGY and TS-GYC had 50% relationship index.

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Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish

Jurnal Biodjati ◽

10.15575/biodjati.v4i2.4741 ◽

2019 ◽

Vol 4 (2) ◽

pp. 204-213

Author(s):

Iskarimah Yolanisa ◽

Ibnu Dwi Buwono ◽

Yuniar Mulyani ◽

Iskandar Iskandar

Keyword(s):

Random Amplified Polymorphic Dna ◽

Clarias Gariepinus ◽

Similarity Index ◽

Transgenic Fish ◽

Fast Growth ◽

Relationship Analysis ◽

Rapd Pcr ◽

Pcr Method ◽

Polymerase Chain ◽

Relationship Of

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.

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Genetic Analysis of Tilapia (Oreochromis niloticus) Nirwana Strain Cultured in Aceh Besar and Wanayasa by Using Random Amplified Polymorphic DNA Method

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v4i430111 ◽

2020 ◽

pp. 1-9

Author(s):

Ayi Yustiati ◽

Reifolnanda Mangappu Hutagalung ◽

Yuli Andriani ◽

Walim Lili ◽

Ibnu Bangkit

Keyword(s):

Genetic Relationship ◽

Oreochromis Niloticus ◽

Dna Isolation ◽

Random Amplified Polymorphic Dna ◽

Geographical Isolation ◽

Genetic Stock ◽

Internal Factors ◽

Rapd Pcr ◽

Aceh Province ◽

Relationship Of

This research was carried out from June to November 2017. This research aims to analyze genetic relationship of tilapia (Oreochromis niloticus) nirwana strain which is cultured in Aceh Besar (Aceh Province) and in Wanayasa (West Java Province) with RAPD-PCR technique. The DNA Isolation Activity of tilapia nirwana strain was carried out in The Biotechnology Laboratory, Building No.4, FPIK UNPAD, Indonesia and the PCR process was carried out in The Microbiology and Biotechnology Laboratory, Building No.3 FPIK UNPAD, Indonesia. The purpose of this research is to determine the level of genetic relationship of tilapia nirwana strain cultured in Aceh Besar and Wanayasa and to find out whether tilapia nirwana strain in Aceh Besar can be used as a genetic stock. The results showed that genetic relationship level of tilapia nirwana strain cultured in Aceh Besar with those cultured in Wanayasa is 67%. The existence of environmental differences results in genetic diversity between Aceh Besar and Wanayasa tilapia nirwana strain. Factors that causing grouping movement are external factors such as: Geographical isolation and habitat fragmentation as well as internal factors such as mutation, natural selection, genetic drift, and gene flow. Tilapia nirwana strain that is cultured in Aceh Besar produce polymorphic bands, so it can be used as a new genetic stock to stabilize the tilapia nirwana strain production.

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Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v8i130183 ◽

2021 ◽

pp. 1-8

Author(s):

Riva Hafidah ◽

Ayi Yustiati ◽

Yuniar Mulyani ◽

Ibnu Bangkit Bioshina Suryadi

Keyword(s):

Genetic Diversity ◽

Random Amplified Polymorphic Dna ◽

Descriptive Analysis ◽

Similarity Index ◽

Quantitative Descriptive Analysis ◽

Rapd Pcr ◽

Result Show ◽

Pcr Method ◽

Quantitative Descriptive

This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR method. The obtained genetic diversity data is used as guide reference for hybridization between four strains. The research was conducted in September 2020 to April 2021 with explorative methods and in qualitative and quantitative descriptive analysis. The research was carried out in biotechnology Laboratory, Fishery and Marine Sciences Faculty and Central Laboratory,Padjadjaran University, Indonesia.Strains of JBD, JBTD, BM obtained fromCilengkrangSubdistrict, Bandung and PG strain obtained from Parung market, Bogor. Primary OPA-03 (AGTCAGCCAC) is used for standard parameters to interpret genetic diversity among four strains of guppy. Based on results, amplification with OPA-03 primary visualize 25 bands that include five polymorphic bands and 20 monomorphic bands. The phylogenetic tree result show that there are two relationship groups. The first group are JBD, JBTD, and BM with similarity index in the range of 80-89%The first group consist two sub groups of relationship. The first sub group are JBD and JBTD with similarity index of 89%. The second sub group is BM with similarity index of 80%. The second group isPG with similarity index of 65.5%.

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Using of RAPD-PCR markers to detect the genetic relationship of number of Bellevalia Lapeyr and Ornithogalum L. species developing in central and northern of Iraq

Journal of Physics Conference Series ◽

10.1088/1742-6596/1879/2/022014 ◽

2021 ◽

Vol 1879 (2) ◽

pp. 022014

Author(s):

Najat Ameen Sa’eed ◽

Akeel Hussein Ali Al-Assie ◽

Naglaa Mustafa Al-Abide

Keyword(s):

Genetic Relationship ◽

Pcr Markers ◽

Rapd Pcr ◽

Relationship Of

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WEEDS IN POTATO CULTURE AND THEIR OUTCOME IN SPREADING OF Alternaria spp.

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2018.6.16 ◽

2018 ◽

Vol 17 (6) ◽

pp. 159-166

Author(s):

Halina Kurzawińska ◽

Stanisław Mazur ◽

Małgorzata Nadziakiewicz ◽

Jacek Nawrocki

Keyword(s):

Chenopodium Album ◽

Potato Cultivar ◽

Cirsium Arvense ◽

Potato Plants ◽

Potato Culture ◽

Rapd Pcr ◽

Microscopic Features ◽

Weed Infestation ◽

Pcr Method ◽

Potato Crops

The aim of this study was to determine whether the weeds accompanying potato crops can be a source of Alternaria spp. causing Alternaria leaf blight and to determine the genetic similarities of Alternaria alternata isolates infecting selected weeds: Chenopodium album, Cirsium arvense and tested potato cultivar. Three-year field experiment was conducted on the potato cultivar ‘Vineta N’. The isolates were classified into different species on the basis of macro- and microscopic features. In each year of the study, A. alternata dominated among the isolated fungi colonizing the leaves of potato plants and the selected weeds. The genetic similarities of A. alternata isolates was determined by the RAPD-PCR method. Tested genetic forms of A. alternata were closely related; only small differences in the pattern of the separated amplification products was evidenced. The dominance of A. alternata on the weeds accompanying potato crops suggests that if weed infestation is extensive, the pathogen is very likely to spread and its population to increase.

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