scholarly journals Turning waste into valuable products: utilization of agroindustrial oily wastes as the low-cost media for microbial lipase production

2021 ◽  
Vol 1918 (5) ◽  
pp. 052028
Author(s):  
F Fibriana ◽  
A Upaichit ◽  
B Cheirsilp
Keyword(s):  
Low Cost ◽  
Lipase Production ◽  
Microbial Lipase

scholarly journals Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

2018 ◽  
Vol 12 (1) ◽  
pp. 189-203 ◽  
Author(s):  
Georgi Dobrev ◽  
Hristina Strinska ◽  
Anelia Hambarliiska ◽  
Boriana Zhekova ◽  
Valentina Dobreva
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Lipase Activity ◽  
Nutrient Medium ◽  
Solid Medium ◽  
Low Cost ◽  
Lipase Production ◽  
Rhizopus Arrhizus ◽  
Agroindustrial Wastes

Background: Rhizopus arrhizus is a potential microorganism for lipase production. Solid-state fermentation is used for microbial biosynthesis of enzymes, due to advantages, such as high productivity, utilization of abundant and low-cost raw materials, and production of enzymes with different catalytic properties. Objective: The objective of the research is optimization of the conditions for lipase production in solid-state fermentation by Rhizopus arrhizus in a nutrient medium, containing agroindustrial wastes. Method: Biosynthesis of lipase in solid-state fermentation by Rhizopus arrhizus was investigated. The effect of different solid substrates, additional carbon and nitrogen source, particles size and moisture content of the medium on enzyme production was studied. Response surface methodology was applied for determination of the optimal values of moisture content and tryptone concentration. A procedure for efficient lipase extraction from the fermented solids was developed. Results: Highest lipase activity was achieved when wheat bran was used as a solid substrate. The addition of 1% (w/w) glucose and 5% (w/w) tryptone to the solid medium significantly increased lipase activity. The structure of the solid medium including particles size and moisture content significantly influenced lipase production. A mathematical model for the effect of moisture content and tryptone concentration on lipase activity was developed. Highest enzyme activity was achieved at 66% moisture and 5% (w/w) tryptone. The addition of the non-ionic surfactant Disponyl NP 3070 in the eluent for enzyme extraction from the fermented solids increased lipase activity about three folds. Conclusion: After optimization of the solid-state fermentation the achieved 1021.80 U/g lipase activity from Rhizopus arrhizus was higher and comparable with the activity of lipases, produced by other fungal strains. The optimization of the conditions and the use of low cost components in solid-state fermentation makes the process economicaly effective for production of lipase from the investigated strain Rhizopus arrhizus.

Olive-mill wastewaters: a promising substrate for microbial lipase production

2006 ◽  
Vol 97 (15) ◽  
pp. 1828-1833 ◽  
Author(s):  
Alessandro D’Annibale ◽  
Giovanni Giovannozzi Sermanni ◽  
Federico Federici ◽  
Maurizio Petruccioli
Keyword(s):  
Lipase Production ◽  
Microbial Lipase ◽  
Olive Mill Wastewaters ◽  
Olive Mill

Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology

2013 ◽  
Vol 34 (15) ◽  
pp. 2259-2266 ◽  
Author(s):  
Mohamed Sellami ◽  
Samiha Kedachi ◽  
Fakher Frikha ◽  
Nabil Miled ◽  
Faouzi Ben Rebah
Keyword(s):  
Design Methodology ◽  
Lipase Production ◽  
Mixture Design ◽  
Growth Media ◽  
Microbial Lipase ◽  
Marine Waste

Use of a low-cost methodology for biodetoxification of castor bean waste and lipase production

2009 ◽  
Vol 44 (5) ◽  
pp. 317-322 ◽  
Author(s):  
Mateus G. Godoy ◽  
Melissa L.E. Gutarra ◽  
Fábio M. Maciel ◽  
Shayany P. Felix ◽  
Juliana V. Bevilaqua ◽  
...  
Keyword(s):  
Castor Bean ◽  
Low Cost ◽  
Lipase Production

scholarly journals Palm Oil Decanter Cake Wastes as Alternative Nutrient Sources and Biomass Support Particles for Production of Fungal Whole-Cell Lipase and Application as Low-Cost Biocatalyst for Biodiesel Production

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1365
Author(s):  
Aran H-Kittikun ◽  
Benjamas Cheirsilp ◽  
Natthapat Sohsomboon ◽  
Darun Binmarn ◽  
Wasu Pathom-aree ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Palm Oil ◽  
Low Cost ◽  
Lipase Production ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Whole Cell ◽  
Nutrient Sources ◽  
Decanter Cake ◽  
Biomass Support Particles

This is the first report on the possible use of decanter cake waste (DCW) from palm oil industry as alternative nutrient sources and biomass support particles for whole-cell lipase production under solid-state fermentation (SSF) by newly isolated fungal Aspergillus sp. MS15 and their application as a low-cost and environment-friendly biocatalyst for biodiesel production. The results found that DCW supplemented with 0.1% K2HPO4, 0.05% MgSO4·7H2O, 1% peptone and 2% urea and pH adjusted to 6.0 was optimal for whole-cell lipase production. The optimal moisture content and fermentation temperature was 60% and 37.5 °C, respectively. Environmentally friendly biodiesel production, through either esterification or transesterification using whole-cell lipase immobilized on DCW as a biocatalyst, was optimized. The optimal reaction temperature for both reactions was 37 °C. The whole-cell lipase effectively esterified oleic acid into >95% biodiesel yield through esterification under optimal water activity at 0.71 and an optimal methanol to oleic acid molar ratio of 2:1, and also effectively transesterified palm oil under optimal water activity at 0.81 and an optimal methanol to oil molar ratio of 3:1. The fuel properties of produced biodiesel are close to the international biodiesel standards. These results have shown the circular utilization of palm oil mill waste for the low-cost production of an effective biocatalyst, and may contribute greatly to the sustainability of renewable bioenergy production.

Microbial lipase production on a polymeric resin

1995 ◽  
Vol 9 (8) ◽  
pp. 597-600 ◽  
Author(s):  
P. Christen ◽  
N. Angeles ◽  
G. Corzo ◽  
A. Farres ◽  
S. Revah
Keyword(s):  
Lipase Production ◽  
Microbial Lipase ◽  
Polymeric Resin

Nitric oxide: a novel inducer for enhancement of microbial lipase production

2016 ◽  
Vol 39 (11) ◽  
pp. 1671-1678 ◽  
Author(s):  
Mesut Taskin ◽  
Yagmur Unver ◽  
Melike Yildiz ◽  
Serkan Ortucu ◽  
Hakan Askin
Keyword(s):  
Nitric Oxide ◽  
Lipase Production ◽  
Microbial Lipase

Decomposition of the metastable beta titanium alloy Ti-15-3

1983 ◽  
Vol 41 ◽  
pp. 264-265
Author(s):  
Y. L. Chen ◽  
S. Fujlshiro
Keyword(s):  
Low Cost ◽  
High Strength ◽  
Alpha Phase ◽  
Thick Sheet ◽  
Omega Phase ◽  
Beta Titanium Alloy ◽  
Beta Titanium ◽  
Beta Matrix ◽  
Metastable Beta ◽  
Very High

Metastable beta titanium alloys have been known to have numerous advantages such as cold formability, high strength, good fracture resistance, deep hardenability, and cost effectiveness. Very high strength is obtainable by precipitation of the hexagonal alpha phase in a bcc beta matrix in these alloys. Precipitation hardening in the metastable beta alloys may also result from the formation of transition phases such as omega phase. Ti-15-3 (Ti-15V- 3Cr-3Al-3Sn) has been developed recently by TIMET and USAF for low cost sheet metal applications. The purpose of the present study was to examine the aging characteristics in this alloy.The composition of the as-received material is: 14.7 V, 3.14 Cr, 3.05 Al, 2.26 Sn, and 0.145 Fe. The beta transus temperature as determined by optical metallographic method was about 770°C. Specimen coupons were prepared from a mill-annealed 1.2 mm thick sheet, and solution treated at 827°C for 2 hr in argon, then water quenched. Aging was also done in argon at temperatures ranging from 316 to 616°C for various times.

Chromic Acid Etching of Polyethylene

1973 ◽  
Vol 31 ◽  
pp. 72-73
Author(s):  
J. D. Muzzy ◽  
R. D. Hester ◽  
J. L. Hubbard
Keyword(s):  
Chromic Acid ◽  
Low Cost ◽  
Acid Etching ◽  
Crystalline Morphology ◽  
Bulk Specimen ◽  
Morphological Studies ◽  
Perfect Form ◽  
Molecular Length ◽  
Lamellar Texture ◽  
Cost Studies

Polyethylene is one of the most important plastics produced today because of its good physical properties, ease of fabrication and low cost. Studies to improve the properties of polyethylene are leading to an understanding of its crystalline morphology. Polyethylene crystallized by evaporation from dilute solutions consists of thin crystals called lamellae. The polyethylene molecules are parallel to the thickness of the lamellae and are folded since the thickness of the lamellae is much less than the molecular length. This lamellar texture persists in less perfect form in polyethylene crystallized from the melt.Morphological studies of melt crystallized polyethylene have been limited due to the difficulty of isolating the microstructure from the bulk specimen without destroying or deforming it.

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