scholarly journals Identifying methicillin-resistant Staphylococcus aureus (MRSA) lung infections in mice via breath analysis using secondary electrospray ionization-mass spectrometry (SESI-MS)

2014 ◽  
Vol 8 (4) ◽  
pp. 041001 ◽  
Author(s):  
Heather D Bean ◽  
Jiangjiang Zhu ◽  
Jackson C Sengle ◽  
Jane E Hill
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Methicillin Resistant ◽  
Secondary Electrospray Ionization ◽  
Lung Infections

scholarly journals Secondary electrospray ionization-mass spectrometry (SESI-MS) breathprinting of multiple bacterial lung pathogens, a mouse model study

2013 ◽  
Vol 114 (11) ◽  
pp. 1544-1549 ◽  
Author(s):  
Jiangjiang Zhu ◽  
Heather D. Bean ◽  
Jaime Jiménez-Díaz ◽  
Jane E. Hill
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Principal Components ◽  
Electrospray Ionization Mass Spectrometry ◽  
Bacterial Pneumonia ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Secondary Electrospray Ionization ◽  
Lung Infections

Bacterial pneumonia is one of the leading causes of disease-related morbidity and mortality in the world, in part because the diagnostic tools for pneumonia are slow and ineffective. To improve the diagnosis success rates and treatment outcomes for bacterial lung infections, we are exploring the use of secondary electrospray ionization-mass spectrometry (SESI-MS) breath analysis as a rapid, noninvasive method for determining the etiology of lung infections in situ. Using a murine lung infection model, we demonstrate that SESI-MS breathprints can be used to distinguish mice that are infected with one of seven lung pathogens: Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae, representing the primary causes of bacterial pneumonia worldwide. After applying principal components analysis, we observed that with the first three principal components (primarily comprised of data from 14 peaks), all infections were separable via SESI-MS breathprinting ( P < 0.0001). Therefore, we have shown the potential of this SESI-MS approach for rapidly detecting and identifying acute bacterial lung infections in situ via breath analysis.

scholarly journals Dinoxin B Withanolide from Datura inoxia Mill as an Effective Phytocompound Against Urinary Tract Infection causing Staphylococcus aureus

2021 ◽  
Vol 14 (3) ◽  
pp. 1707-1720
Author(s):  
Ruby George ◽  
Priti Mathur ◽  
Chandni Tandon
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Binding Protein ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Penicillin Binding Protein ◽  
Time Kill ◽  
Datura Inoxia

Background: Dinoxin B Withanolide was isolated from Datura inoxia and identified with its cytotoxic activity. But its antibacterial properties are not yet evaluated. We have previously reported the broad-spectrum antibacterial property of Dinoxin B Withanolide extracted from D.inoxia on standard strains. Objective: This research has focused to evaluate the efficacy of Dinoxin B Withanolide against infectious Staphylococcus aureus, including resistant strains. Methods: Electrospray Ionization-Mass Spectrometry is used to depict the presence of Dinoxin B withanolide from the chromatographic ethanolic leaf fraction. Antibacterial activity of different concentrations of Dinoxin B(12500-100000 μg/ml) was assessed using the agar diffusion, macro broth dilution, and time-kill assay methods. Docking studies and Drug likeness properties were analyzed. Result: Electrospray Ionization-Mass Spectrometry depicted the presence of Dinoxin B. All the isolates were susceptible to Dinoxin B within the range of 15±0.5mm to 24±0.5mm, and the bacteria were susceptible at a concentration rate of ≤12.5mg/ml. Time-kill assay showed that 25mg/ml of Dinoxin B displayed the highest inhibitory activity after four hours. The MBC values were compatible with the cidal concentration as seen in the time-kill study's growth curve. Computer-aided techniques resulted in a good Docking score towards Quorum-signaling Sar A protein (-7.82)and Penicillin Binding Protein(-6.9). Conclusion: Dinoxin B with its bactericidal properties and significant affinity towards Quorum-signaling Sar A protein and Penicillin Binding Protein can be considered as an effective bioactive compound against Methicillin Resistance Staphylococcus aureus.

Sign in / Sign up

Export Citation Format

Share Document