scholarly journals Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization – mass spectrometry

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Lukas Bregy ◽  
Annick R. Müggler ◽  
Pablo Martinez-Lozano Sinues ◽  
Diego García-Gómez ◽  
Yannick Suter ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Human Saliva ◽  
Oral Bacteria ◽  
In Vitro Cultures ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Secondary Electrospray Ionization

scholarly journals Increased Glutathionyl Hemoglobin in Diabetes Mellitus and Hyperlipidemia Demonstrated by Liquid Chromatography/Electrospray Ionization-Mass Spectrometry

2000 ◽  
Vol 46 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Toshimitsu Niwa ◽  
Chika Naito ◽  
Abdul Hassan Mohammed Mawjood ◽  
Kiyohiro Imai
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Healthy Subjects ◽  
Oxygen Affinity ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass

Abstract Background: Erythrocytes contain a large amount of glutathione (GSH), which protects cells from oxidative injury. The purpose of this study was to examine whether hemoglobin (Hb) is modified with glutathione by oxidation of the thiol groups in diabetes mellitus and hyperlipidemia, and to determine the oxygen affinity of glutathionyl Hb. Methods: Hb samples obtained from patients with type 2 diabetes, patients with hyperlipidemia, and healthy subjects were analyzed by liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS). Glutathionyl Hb was synthesized in vitro by incubating Hb with GSH. The oxygen affinity of glutathionyl Hb was determined by measuring its oxygen dissociation curve. Results: We first demonstrated that the concentration of glutathionyl Hbβ chains is markedly increased in the diabetic patients and hyperlipidemic patients compared with healthy subjects. The in vitro synthesis of glutathionyl Hb by incubation of Hb with GSH was enhanced by adding H2O2, a reactive oxygen species, into the incubation solution. The glutathionyl Hb prepared in vitro by incubating Hb with GSH showed a marked increase in oxygen affinity and a marked decrease in the Hill coefficient compared with Hb incubated without GSH. Conclusions: Glutathionyl Hb may be useful as a clinical marker of oxidative stress. The increased concentrations of glutathionyl Hb with high oxygen affinity and low cooperativity in diabetes and hyperlipidemia may lead to reduced tissue oxygen delivery.

scholarly journals Electrospray ionization mass spectrometry combined with the ultra high performance liquid chromatography in the analysis of in vitro formation of chlorophyll complexes with copper and zinc

2014 ◽  
Vol 79 (6) ◽  
pp. 689-706 ◽  
Author(s):  
Jelena Zvezdanovic ◽  
Sanja Petrovic ◽  
Dejan Markovic ◽  
Tatjana Andjelkovic ◽  
Darko Andjelkovic
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
High Performance ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Central Type

The aim of this work was to give more accurate insight in the interaction of major photosynthesis pigment, chlorophyll (Chl), with copper (II) and zinc (II) in solution by flow injection analysis with electrospray ionization mass spectrometry (FIA-ESI-MS) method, as well as combined with ultra high performance liquid chromatography with DAD detection (UHPLC-DAD). These interactions may potentially, but not necessarily lead to formation of Cu-Chl and Zn-Chl complexes of two different types, which has a lot of - at least - disfunctional implications in plant world. The results based on analysis of full-scan and MS/MS spectra - with or without the UHPLC chromatograms - confirm formation of ?central type? Cu-Chl complex, and ?central type? Zn-Chl complex as well, along with a proof for formation of ?peripheral? Zn-Chl complex, the latter one originating from a very week coordinative interaction at the edge of Chl structure. The used techniques appeared to be an efficient and reliable tool for studying formation and stability of heavy metals complexes with chlorophyll, at least in vitro, with a considerable possibility for assessment of real bio-environmental behavior.

scholarly journals Secondary electrospray ionization-mass spectrometry (SESI-MS) breathprinting of multiple bacterial lung pathogens, a mouse model study

2013 ◽  
Vol 114 (11) ◽  
pp. 1544-1549 ◽  
Author(s):  
Jiangjiang Zhu ◽  
Heather D. Bean ◽  
Jaime Jiménez-Díaz ◽  
Jane E. Hill
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Principal Components ◽  
Electrospray Ionization Mass Spectrometry ◽  
Bacterial Pneumonia ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Secondary Electrospray Ionization ◽  
Lung Infections

Bacterial pneumonia is one of the leading causes of disease-related morbidity and mortality in the world, in part because the diagnostic tools for pneumonia are slow and ineffective. To improve the diagnosis success rates and treatment outcomes for bacterial lung infections, we are exploring the use of secondary electrospray ionization-mass spectrometry (SESI-MS) breath analysis as a rapid, noninvasive method for determining the etiology of lung infections in situ. Using a murine lung infection model, we demonstrate that SESI-MS breathprints can be used to distinguish mice that are infected with one of seven lung pathogens: Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae, representing the primary causes of bacterial pneumonia worldwide. After applying principal components analysis, we observed that with the first three principal components (primarily comprised of data from 14 peaks), all infections were separable via SESI-MS breathprinting ( P < 0.0001). Therefore, we have shown the potential of this SESI-MS approach for rapidly detecting and identifying acute bacterial lung infections in situ via breath analysis.

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