scholarly journals Antiviral Activity of Isobutyl Gallate to Dengue Virus Serotype 2 In Vitro

2019 ◽  
Vol 251 ◽  
pp. 012018 ◽  
Author(s):  
B E Dewi ◽  
M Angelina ◽  
F Nuwwaaridya ◽  
H Desti ◽  
T M Sudiro
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals In vitro antiviral activity of spirotetronate compounds against dengue virus serotype 2

2019 ◽  
Vol 65 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Jirayut Euanorasetr ◽  
Bungonsiri Intra ◽  
Nutthanit Thunmrongsiri ◽  
Jitra Limthongkul ◽  
Sukathida Ubol ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Antiviral Activity of Novel Quinoline Derivatives against Dengue Virus Serotype 2

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 672 ◽  
Author(s):  
Carolina de la Guardia ◽  
David Stephens ◽  
Hang Dang ◽  
Mario Quijada ◽  
Oleg Larionov ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Quinoline Derivatives ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Drug of Action Cassia Alata Leaves Extract as Antiviral to Dengue Virus Serotype-2 in vitro

2020 ◽  
Vol 12 (4) ◽  
pp. 864-871
Author(s):  
Marissa Angelina ◽  
Muhammad Hanafi ◽  
Franciscus D Suyatna ◽  
Beti Ernawati Dewi
Keyword(s):  
Dengue Virus ◽  
Cassia Alata ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Taraxacum officinale and Urtica dioica extracts inhibit dengue virus serotype 2 replication in vitro

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
María R. Flores-Ocelotl ◽  
Nora H. Rosas-Murrieta ◽  
Diego A. Moreno ◽  
Verónica Vallejo-Ruiz ◽  
Julio Reyes-Leyva ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Taraxacum Officinale ◽  
Urtica Dioica ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Comparative Evaluation of Permissiveness to Dengue Virus Serotype 2 Infection in Primary Rodent Macrophages

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Jeanette Prada-Arismendy ◽  
Verónica Rincón ◽  
Jaime E. Castellanos
Keyword(s):  
Dengue Virus ◽  
Comparative Evaluation ◽  
Molecular Mechanisms ◽  
Rodent Species ◽  
Oligoadenylate Synthetase ◽  
Virus Serotype ◽  
Mouse Cells ◽  
Dengue Virus Serotype 2 ◽  
Denv2 Infection

Infection with dengue virus presents a broad clinical spectrum, which can range from asymptomatic cases to severe cases that are characterised by haemorrhagic syndrome and/or shock. The reason for such variability remains unknown. This work evaluated thein vitropermissiveness of mouse, rat, hamster and guinea pig macrophages to infection by dengue virus 2 (DENV2). The results established that macrophages derived from the BALB/c mouse strain showed higher permissiveness to DENV2 infection than macrophages from other rodent species, although all rodent species studied had the C820T mutation in the oligoadenylate synthetase 1b gene, indicating no relationship to the differentin vitrosusceptibilities of mouse cells at this locus. Other molecular mechanisms related to flavivirus susceptibility remain to be explored.

scholarly journals Non Fc receptor-mediated infection of human macrophages by dengue virus serotype 2

2002 ◽  
Vol 83 (5) ◽  
pp. 1123-1130 ◽  
Author(s):  
M. M. Bertha Moreno-Altamirano ◽  
F. Javier Sánchez-García ◽  
M. Lourdes Muñoz
Keyword(s):  
Dengue Virus ◽  
Polyclonal Antibodies ◽  
Primary Cultures ◽  
Human Monocyte ◽  
Target Cells ◽  
Human Macrophages ◽  
Virus Serotype ◽  
Molecular Masses ◽  
Dengue Virus Serotype 2

Four human monocyte-derived macrophage membrane proteins, with apparent molecular masses of 27, 45, 67 and 87 kDa, were identified as possible receptors for dengue virus serotype 2 (DEN-2) (Mexican isolate 200787/1983), based on affinity chromatography, immunofluorescence, virus overlay protein-binding assays and Western blotting. Additionally, mouse polyclonal antibodies raised against each of the four proteins were capable of partially inhibiting in vitro DEN-2 infection of monocyte-macrophages, thus supporting the notion of a role for such proteins as DEN-2 receptors. Parallel studies were carried out using the human promonocytic U-937 cell line, both as undifferentiated cells and as monocyte-like phorbol myristate acetate (PMA)-differentiated cells, as target cells. Whereas interaction between DEN-2 and undifferentiated U-937 cells was almost negligible, PMA-differentiated U-937 cells were shown to harbour putative receptors (with molecular masses of 45 and 67 kDa) for DEN-2, similar to those found in human monocyte-derived macrophages. To our knowledge, this is the first report that describes putative receptors for DEN-2 in primary cultures of human macrophages.

scholarly journals Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Kavithambigai Ellan ◽  
Ravindran Thayan ◽  
Jegadeesh Raman ◽  
Kazuya I. P. J. Hidari ◽  
Norizah Ismail ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dengue Virus ◽  
Viral Infection ◽  
Schizophyllum Commune ◽  
Health Concern ◽  
Composition Analysis ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2 ◽  
Mushroom Extracts

Abstract Background Dengue is a mosquito-borne viral infection that has become a major public health concern worldwide. Presently, there is no specific vaccine or treatment available for dengue viral infection. Methods Lignosus rhinocerotis, Pleurotus giganteus, Hericium erinaceus, Schizophyllum commune and Ganoderma lucidium were selected for evaluation of their in-vitro anti-dengue virus serotype 2 (DENV-2) activities. Hot aqueous extracts (HAEs), ethanol extracts (EEs), hexane soluble extracts (HSEs), ethyl acetate soluble extracts (ESEs) and aqueous soluble extracts (ASEs) were prepared from the selected mushrooms. The cytotoxic effects of the extracts were evaluated by the MTT assay. The anti-DENV-2 activities of the extracts were evaluated in three different assays: simultaneous, attachment and penetration assays were perfomed using plaque reduction assays and RT-qPCR assays. The effect of the addition time on viral replication was assessed by the time of addition assay, and a virucidal assay was carried out to evaluate the direct effect of each mushroom extract on DENV-2. The chemical composition of glucans, and the protein and phenolic acid contents in the extracts were estimated. Results We found that the HAEs and ASEs of L. rhinocerotis, P. giganteus, H. erinaceus and S. commune were the least toxic to Vero cells and showed very prominent anti-DENV2 activity. The 50% inhibitory concentration (IC50) values of the ASEs ranged between 399.2–637.9 μg/ml, while for the HAEs the range was 312.9–680.6 μg/ml during simultaneous treatment. Significant anti-dengue activity was also detected in the penetration assay of ASEs (IC50: 226.3–315.4 μg/ml) and HAEs (IC50: 943.1–2080.2 μg/ml). Similarly, we observed a marked reduction in the expression levels of the ENV and NS5 genes in the simultaneous and penetration assays of the ASEs and HAEs. Time-of-addition experiments showed that the highest percent of anti-DENV2 activity was observed when the mushroom extracts were added immediately after virus adsorption. None of the extracts exhibited virucidal effect. Chemical composition analysis showed that the major components in the mushroom HAEs and ASEs were glucan (beta D-glucan) and proteins, however, there was no significant correlation between the anti-dengue activity and the concentration of glucans and proteins. Conclusion These findings demonstrated the potential of mushroom extracts as anti-dengue therapeutic agents with less toxic effects.

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