Phagocytes response to dietary probiotics SEAL and Aeromonas hydrophila infection in red tilapia (Oreochromis sp.)

Abstract Background Streptococcosis and Motile Aeromonad Septicemia (MAS) are important diseases of tilapia, Oreochromis spp. and causes huge economic losses in aquaculture globally. The feed-based vaccination may be an alternative to minimize major infectious diseases in tilapia. Thus, this study aims to evaluate the haemato-immunological responses and effectiveness of a newly developed feed-based killed bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila in hybrid red tilapia. A total of 495 hybrid red tilapia of 61.23±4.95 g were distributed into 5 groups (each with triplicate). The fish were immunized orally through bivalent (combined S. iniae and A. hydrophila ) spray vaccine (BS group), bivalent formulate vaccine (BF group), monovalent S. iniae vaccine (MS group), monovalent A. hydrophila vaccine (MA group) and unvaccinated as a control group. The vaccine was orally administered on days 0, 14 and 42 applied feed-based bacterin at 5% body weight. The blood and spleen samples were collected from all groups on 7, 21 and 49 days post-vaccination, and also 96 hours post-infection to assess their haemato-immune responses. Results Compared with the unvaccinated group, leukocyte, lymphocytes, monocytes, granulocytes counts in vaccinated groups were significantly ( P < 0.05) increased on 21, 49 days post-vaccination and also 96 hours post-infection, while erythrocytes, haemoglobin and haematocrit in vaccinated groups were significantly ( P < 0.05) enhanced only 96 hours post-infection. Additionally, the lysozyme and phagocytic activity and, serum antibody (IgM) were significantly higher ( P < 0.05) against S. iniae and A. hydrophila in vaccinated groups compared to the unvaccinated group in the pre- and post-infection. Results from the challenge through co-infection with S. iniae and A. hydrophila showed the relative percent survival (RPS) in BF group was 76.37±5.14%, which had the capacity to induce significant protection ( P < 0.05) compared to others groups. Conclusions This study demonstrates the bivalent formulate (BF) group could elicit significant non-specific and specific immunological responses with higher protection in hybrid red tilapia. In addition, this newly developed feed-based bivalent vaccination can be a promising technique for effective and large scale fish immunization in the aquaculture industry.

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The Influence of Binahong (Anredera cordifolia) Leaf Powder Addition to Feed on the Prevalence and Survival Rate of Red Tilapia (Oreochromis niloticus) seeds Infected by Aeromonas hydrophilla Bacteria

Omni-Akuatika ◽

10.20884/1.oa.2020.16.3.846 ◽

2020 ◽

Vol 16 (3) ◽

pp. 144

Author(s):

Juliana Juliana ◽

Yuniarti Koniyo ◽

Citra Panigoro

Keyword(s):

Survival Rate ◽

Aeromonas Hydrophila ◽

Total Body Weight ◽

Fish Feed ◽

Red Tilapia ◽

Leaf Powder ◽

Powder Addition ◽

Total Body ◽

Main Factor

Feed is one main factor in aquaculture activities which influences the quantity and quality of aquaculture commodities. Feed can also increase the cultivated fish survivability to prevent from fish diseases. Binahong leaf powder is one material added in the fish feed making which contains active compounds functioning as antibacterial agent. This study did aim at examining the influence of Binahong leaf powder addition to feed on the prevalence and survival rate of Red Tilapia seeds infected by Aeromonas hydrophila bacteria. This research employed an experimental method using a randomized complete design with four treatments. Each treatment consisted of different Binahong leaf powder dosage used as the material in feed making with treatment A (5%), treatment B (7%), treatment C (9%), and treatment D (control). The test animals were ± 5 cm red tilapia seeds infected by Aeromonas hydrophila bacteria. Feeding was given twice, in the morning and evening with the feeding rate of 10% per day of the total body weight. The research results showed that the addition of Binahong leaf powder did not influence the prevalence and survival rate of red tilapia seeds infected by Aeromonas hydrophila bacteria. Keywords: Aeromonas hydrophila, Bacteria, Binahong, Disease, Feed

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Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

10.21203/rs.2.23474/v2 ◽

2020 ◽

Author(s):

Md. Shirajum Monir ◽

Md Sabri bin Mohd Yusoff ◽

Zarirah binti Mohamed Zulperi ◽

Hasliza binti Abu Hassim ◽

Aslah Mohamad ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Post Infection ◽

Serum Antibody ◽

Economic Losses ◽

Control Group ◽

Streptococcus Iniae ◽

Post Vaccination ◽

Bivalent Vaccine ◽

Red Tilapia ◽

Immunological Responses

Abstract Background Streptococcosis and Motile Aeromonad Septicemia (MAS) are important diseases of tilapia, Oreochromis spp. and causes huge economic losses in aquaculture globally. The feed-based vaccination may be an alternative to minimize major infectious diseases in tilapia. Thus, this study aims to evaluate the haemato-immunological responses and effectiveness of a newly developed feed-based killed bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila in hybrid red tilapia. A total of 495 hybrid red tilapia of 61.23±4.95 g were distributed into 5 groups (each with triplicate). The fish were immunized orally through bivalent (combined S. iniae and A. hydrophila ) spray vaccine (BS group), bivalent formulate vaccine (BF group), monovalent S. iniae vaccine (MS group), monovalent A. hydrophila vaccine (MA group) and unvaccinated as a control group. The vaccine was orally administered on days 0, 14 and 42 applied feed-based bacterin at 5% body weight. The blood and spleen samples were collected from all groups on 7, 21 and 49 days post-vaccination, and also 96 hours post-infection to assess their haemato-immune responses. Results Compared with the unvaccinated group, leukocyte, lymphocytes, monocytes, granulocytes counts in vaccinated groups were significantly ( P < 0.05) increased on 21, 49 days post-vaccination and also 96 hours post-infection, while erythrocytes, haemoglobin and haematocrit in vaccinated groups were significantly ( P < 0.05) enhanced only 96 hours post-infection. Additionally, the lysozyme and phagocytic activity and, serum antibody (IgM) were significantly higher ( P < 0.05) against S. iniae and A. hydrophila in vaccinated groups compared to the unvaccinated group in the pre- and post-infection. Results from the challenge through co-infection with S. iniae and A. hydrophila showed the relative percent survival (RPS) in BF group was 76.37±5.14%, which had the capacity to induce significant protection ( P < 0.05) compared to others groups. Conclusions This study demonstrates the bivalent formulate (BF) group could elicit significant non-specific and specific immunological responses with higher protection in hybrid red tilapia. In addition, this newly developed feed-based bivalent vaccination can be a promising technique for effective and large scale fish immunization in the aquaculture industry.

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Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

BMC Veterinary Research ◽

10.1186/s12917-020-02443-y ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Md Shirajum Monir ◽

Sabri bin Mohd Yusoff ◽

Zarirah binti Mohamed Zulperi ◽

Hasliza binti Abu Hassim ◽

Aslah Mohamad ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Oreochromis Mossambicus ◽

Streptococcus Iniae ◽

Bivalent Vaccine ◽

Red Tilapia ◽

Immunological Responses

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Efficacy of Formalin-killed Aeromonas hydrophila and Streptococcus sp. Vaccine in Red Tilapia

Our Nature ◽

10.3126/on.v8i1.4333 ◽

1970 ◽

Vol 8 (1) ◽

pp. 231-240 ◽

Cited By ~ 1

Author(s):

S. Prasad ◽

N. Areechon

Keyword(s):

Aeromonas Hydrophila ◽

Humoral Response ◽

Secondary Response ◽

Separate Experiment ◽

Post Challenge ◽

Red Tilapia ◽

Antibody Titers ◽

High Level ◽

Direct Immersion ◽

Stimulation Of

Humoral response in red tilapia against formalin-killed Aeromonas hydrophila and Streptococ cus sp. vaccine administered by intraperitoneal injection was evaluated.The result indicated that A. hydrophila vaccine induced significantly differed (P<0.05) high mean peak antibody titers of 925.87±467.92 and 4983.47±1832.74 in both primary and secondary immune response, respectively. However specific antibody produced by red tilapia in response to administration of Streptococcus sp. vaccine revealed only weak secondary response of 101.33±45.38. In separate experiment, relative protection in red tilapia immunized with A. hydrophila and Streptococcus sp. vaccine was conducted. Immunization were done by direct immersion for 1 hr in vaccine suspension and then challenged 2 weeks after by immersing fingerlings for 6 hr with virulent A. hydrophila and Streptococcus sp. Percent cumulative mortality in vaccinated and unvaccinated groups was compared after 14 days of post challenge. Red tilapia immunized by A. hydrophila vaccine demonstrated a particularly high level of immunity (76.67%) compared with unvaccinated (43.33%). Streptococcus sp. vaccine greatly reduced the mortality in vaccinated (31.67%) compared with unvaccinated fish (55%) but these differences in mortality were insignificant (P>0.05). Results from this study indicated the importance of vaccine for increasing disease resistance against A. hydrophila and Streptococcus sp infection by stimulation of specific humoral immunity. However the most important factor must be the method of vaccine administration which should be effective and applicable to farm scale.DOI: 10.3126/on.v8i1.4333

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Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

10.21203/rs.2.23474/v3 ◽

2020 ◽

Author(s):

Md. Shirajum Monir ◽

Md Sabri bin Mohd Yusoff ◽

Zarirah binti Mohamed Zulperi ◽

Hasliza binti Abu Hassim ◽

Aslah Mohamad ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Post Infection ◽

Serum Antibody ◽

Economic Losses ◽

Control Group ◽

Streptococcus Iniae ◽

Post Vaccination ◽

Bivalent Vaccine ◽

Red Tilapia ◽

Immunological Responses

Abstract Background: Streptococcosis and Motile Aeromonad Septicemia (MAS) are important diseases of tilapia, Oreochromis spp. and causes huge economic losses in aquaculture globally. The feed-based vaccination may be an alternative to minimize major infectious diseases in tilapia. Thus, this study aims to evaluate the haemato-immunological responses and effectiveness of a newly developed feed-based killed bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila in hybrid red tilapia. A total of 495 hybrid red tilapia of 61.23±4.95 g were distributed into 5 groups (each with triplicate). The fish were immunized orally through bivalent (combined S. iniae and A. hydrophila) spray vaccine (BS group), bivalent formulate vaccine (BF group), monovalent S. iniae vaccine (MS group), monovalent A. hydrophila vaccine (MA group) and unvaccinated as a control group. The vaccine was orally administered on days 0, 14 and 42 applied feed-based bacterin at 5% body weight. The blood and spleen samples were collected from all groups on 7, 21 and 49 days post-vaccination, and also 96 hours post-infection to assess their haemato-immune responses. Results: Compared with the unvaccinated group, leukocyte, lymphocytes, monocytes, granulocytes counts in vaccinated groups were significantly (P < 0.05) increased on 21, 49 days post-vaccination and also 96 hours post-infection, while erythrocytes, haemoglobin and haematocrit in vaccinated groups were significantly (P < 0.05) enhanced only 96 hours post-infection. Additionally, the lysozyme and phagocytic activity and, serum antibody (IgM) were significantly higher (P < 0.05) against S. iniae and A. hydrophila in vaccinated groups compared to the unvaccinated group in the pre- and post-infection. Results from the challenge through co-infection with S. iniae and A. hydrophila showed the relative percent survival (RPS) in BF group was 76.67±4.71%, which had the capacity to induce significant protection (P < 0.05) compared to others groups. Conclusions: This study demonstrates the bivalent formulate (BF) group could elicit significant non-specific and specific immunological responses with higher protection in hybrid red tilapia. In addition, this newly developed feed-based bivalent vaccination can be a promising technique for effective and large scale fish immunization in the aquaculture industry.

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Haemato-immunological responses and effectiveness of feed-based bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila infections in hybrid red tilapia (Oreochromis mossambicus × O. niloticus)

10.21203/rs.2.23474/v4 ◽

2020 ◽

Author(s):

Md. Shirajum Monir ◽

Md Sabri bin Mohd Yusoff ◽

Zarirah binti Mohamed Zulperi ◽

Hasliza binti Abu Hassim ◽

Aslah Mohamad ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Post Infection ◽

Serum Antibody ◽

Economic Losses ◽

Control Group ◽

Streptococcus Iniae ◽

Post Vaccination ◽

Bivalent Vaccine ◽

Red Tilapia ◽

Immunological Responses

Abstract Background: Streptococcosis and Motile Aeromonad Septicemia (MAS) are important diseases of tilapia, Oreochromis spp. and causes huge economic losses in aquaculture globally. The feed-based vaccination may be an alternative to minimize major infectious diseases in tilapia. Thus, this study aims to evaluate the haemato-immunological responses and effectiveness of a newly developed feed-based killed bivalent vaccine against Streptococcus iniae and Aeromonas hydrophila in hybrid red tilapia. A total of 495 hybrid red tilapia of 61.23±4.95 g were distributed into 5 groups (each with triplicate). The fish were immunized orally through bivalent (combined S. iniae and A. hydrophila) spray vaccine (BS group), bivalent formulate vaccine (BF group), monovalent S. iniae vaccine (MS group), monovalent A. hydrophila vaccine (MA group) and unvaccinated as a control group. The vaccine was orally administered on days 0, 14 and 42 applied feed-based bacterin at 5% body weight. The blood and spleen samples were collected from all groups on 7, 21 and 49 days post-vaccination, and also 96 hours post-infection to assess their haemato-immune responses. Results: Compared with the unvaccinated group, leukocyte, lymphocytes, monocytes, granulocytes counts in vaccinated groups were significantly (P < 0.05) increased on 21, 49 days post-vaccination and also 96 hours post-infection, while erythrocytes, haemoglobin and haematocrit in vaccinated groups were significantly (P < 0.05) enhanced only 96 hours post-infection. Additionally, the lysozyme and phagocytic activity and, serum antibody (IgM) were significantly higher (P < 0.05) against S. iniae and A. hydrophila in vaccinated groups compared to the unvaccinated group in the pre- and post-infection. Results from the challenge through co-infection with S. iniae and A. hydrophila showed the relative percent survival (RPS) in BF group was 76.67±4.71%, which had the capacity to induce significant protection (P < 0.05) compared to others groups. Conclusions: This study demonstrates the bivalent formulate (BF) group could elicit significant non-specific and specific immunological responses with higher protection in hybrid red tilapia. In addition, this newly developed feed-based bivalent vaccination can be a promising technique for effective and large scale fish immunization in the aquaculture industry.

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