scholarly journals Genetic Variation Among Three Zea Mays L Cultivars In Iraq

2021 ◽  
Vol 910 (1) ◽  
pp. 012013
Author(s):  
Faten Khaleel ◽  
Janan saeed
Keyword(s):  
Polymerase Chain Reaction ◽  
Zea Mays ◽  
Genetic Variation ◽  
Molecular Marker ◽  
Zea Mays L ◽  
Conventional Polymerase Chain Reaction ◽  
Schematic Diagram ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Ampli Fication

Abstract Maize is a very fertile and widely environmental grown crop and it globally cultivated. The purpose of our research was to determine genetic variation among three Zea mays L. cultivars (Al maha, Drachma and Talar F-1). The primer ITS1 and ITS4 used as a molecular marker in a conventional Polymerase Chain Reaction (PCR) with a 290 bp ampli- fication outcome. The nucleotide sequences of amplification products were analyzed, sequence alignment was significantly revealed which confirming Zea mays diagnosis. Furthermore, analysis of genetic relationship revealed a neighboring relationship between Talar F-1 and Almaha cultivars(93),whereas phylogeny schematic diagram clearly showed presence of Drachma cultivar in other cluster(77).

scholarly journals Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
J. Albertyn ◽  
K.M. Tajbhai ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Disease Virus ◽  
Common Disease ◽  
Sample Collection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus ◽  
Viral Isolates

Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.

scholarly journals Detection of MYCN Amplification in Serum DNA Using Conventional Polymerase Chain Reaction

2016 ◽  
Vol 31 (9) ◽  
pp. 1392 ◽  
Author(s):  
Youngeun Ma ◽  
Ji Won Lee ◽  
Soo Jin Park ◽  
Eun Sang Yi ◽  
Young Bae Choi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Conventional Polymerase Chain Reaction ◽  
Mycn Amplification ◽  
Chain Reaction ◽  
Serum Dna ◽  
Polymerase Chain

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

Apolipoprotein E polymorphism determined by restriction enzyme analysis of DNA amplified by polymerase chain reaction: convenient alternative to phenotyping by isoelectric focusing

1990 ◽  
Vol 36 (12) ◽  
pp. 2087-2092 ◽  
Author(s):  
K Kontula ◽  
K Aalto-Setälä ◽  
T Kuusi ◽  
L Hämäläinen ◽  
A C Syvänen
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Apolipoprotein E ◽  
Isoelectric Focusing ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Variant Form ◽  
Chain Reaction ◽  
Apo E ◽  
Polymerase Chain

Abstract Three common alleles determine six apolipoprotein E (apo E) phenotypes that are associated with variations in serum cholesterol in the population. This genetic variation results from single nucleotide alterations at two DNA loci encoding the amino acid residues 112 and 158 of apo E. We compared results of apo E phenotyping carried out by isoelectric focusing with those of apo E genotyping accomplished by direct DNA analysis. In the latter, the target DNA was amplified by the polymerase chain reaction (PCR) and subsequently analyzed by digestion with the restriction enzyme Hha I, followed by polyacrylamide gel electrophoresis of the cleavage products. With one exception, these two techniques yielded similar results from all 40 samples tested. In addition, a rare variant form of apo E (phenotype E1) was analyzed separately and incorrectly diagnosed as E2 by the Hha I digestion method; the anticipated mutation in the codon 127 was, however, confirmed by demonstration of a new Taq I restriction site in this variant gene. These data confirm that the common isoforms of apo E usually arise from genetic variation of the codons 112 and 158 and demonstrate the feasibility of the PCR technique in apo E genotyping.

Sign in / Sign up

Export Citation Format

Share Document