Fluorescent probes sensitive to changes in the cholesterol-to-phospholipids molar ratio in human platelet membranes during atherosclerosis

2016 ◽  
Vol 4 (3) ◽  
pp. 034013 ◽  
Author(s):  
Yevgen Posokhov
Keyword(s):  
Fluorescent Probes ◽  
Human Platelet ◽  
Molar Ratio ◽  
Platelet Membranes

The Uptake of Adenine by Isolated Human Platelet Membranes

1974 ◽  
Vol 32 (02/03) ◽  
pp. 457-464
Author(s):  
Paul C. French ◽  
Jan J. Sixma ◽  
Holm Holmsen
Keyword(s):  
Human Platelet ◽  
Facilitated Diffusion ◽  
Adenine Phosphoribosyltransferase ◽  
Ph Optimum ◽  
Intact Cells ◽  
Platelet Membranes ◽  
Group Translocation ◽  
Triton X

SummaryAdenine uptake into isolated platelet membranes had about the same Km (151 ± 21 • 9 nM) as uptake into intact cells (159 ± 21 nM) and was also competitively inhibited by papaverine and hypoxanthine. No uptake occurred at 0° and accumulated adenine was converted to AMP. AMP was not firmly bound to protein as judged by chromatography of triton X-100 solubilized membranes on Sephadex G25. The pH optimum for adenine uptake was at pH 5-5. Exogenous 5-phosphoribosyl-l-pyrophos- phate strongly stimulated uptake. These data may be explained by uptake of adenine by facilitated diffusion followed by conversion to AMP by adenine phosphoribosyltransferase but group translocation cannot be entirely excluded.

Development of an affinity ligand for purification of .alpha.2-adrenoceptors from human platelet membranes

1984 ◽  
Vol 27 (7) ◽  
pp. 918-921 ◽  
Author(s):  
R. M. DeMarinis ◽  
A. J. Krog ◽  
D. H. Shah ◽  
J. Lafferty ◽  
K. G. Holden ◽  
...  
Keyword(s):  
Human Platelet ◽  
Affinity Ligand ◽  
Platelet Membranes

Additions and Corrections-Development of an Affinity Ligand for Purification of Adrenoceptors from Human Platelet Membranes

1984 ◽  
Vol 27 (12) ◽  
pp. 1753-1753
Author(s):  
R DeMarinis ◽  
A Krog ◽  
D Shah ◽  
J Lafferty ◽  
K Holden ◽  
...  
Keyword(s):  
Human Platelet ◽  
Affinity Ligand ◽  
Platelet Membranes

The Ca2+ uptake and the hydrolysis of various nucleotide triphosphates by human platelet membranes

1984 ◽  
Vol 773 (2) ◽  
pp. 325-328 ◽  
Author(s):  
Menno De Metz ◽  
Jocelyne Enouf ◽  
Marilyne Lebret ◽  
Sylviane Lévy-Tolédano
Keyword(s):  
Human Platelet ◽  
Platelet Membranes ◽  
Nucleotide Triphosphates ◽  
Hydrolysis Of

Characterisation of [3H]lysergic acid diethylamide binding to a 5-hydroxytryptamine receptor on human platelet membranes

1984 ◽  
Vol 97 (1-2) ◽  
pp. 87-93 ◽  
Author(s):  
David P. Geaney ◽  
Michael Schächter ◽  
J.Martin Elliot ◽  
David G. Grahame-Smith
Keyword(s):  
Human Platelet ◽  
Lysergic Acid ◽  
Lysergic Acid Diethylamide ◽  
Platelet Membranes
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