Efficacy of Dendritic Cell Generation for Clinical Use: Recovery and Purity of Monocytes and Mature Dendritic Cells after Immunomagnetic Sorting or Adherence Selection of CD14+ Starting Populations

2003 ◽  
Vol 12 (3) ◽  
pp. 289-299 ◽  
Author(s):  
Friederike Meyer-Wentrup ◽  
Stefan Burdach
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Cell Generation ◽  
Clinical Use ◽  
Immunomagnetic Sorting ◽  
Selection Of

scholarly journals Effect of sampling media and culture conditions on dendritic cell generation: ion depleted plasma negatively influences the survival of monocyte-derived dendritic cells

2016 ◽  
Author(s):  
Zuzana Parackova ◽  
Jana Kayserova ◽  
Anna Sediva
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Autoimmune Disease ◽  
Culture Media ◽  
Culture Conditions ◽  
Cell Generation ◽  
Autologous Serum ◽  
Blood Collection ◽  
Bivalent Ions ◽  
Successful Experiment

Autologous serum or plasma are often used as a supplementation in culture media for autoimmune disease studies. They contain many biologically and immunologically active molecules that might be involved in the development of these diseases. We examined the effect of different cultivating media supplemented with autologous serum and two types of plasma obtained from heparin and EDTA coated tubes on monocyte-derived dendritic cells viability. Our results show that medium with plasma from EDTA coated tubes is unsuitable for cultivation of myeloid cells due to the absence of bivalent ions and that the type of sampling tubes used for blood collection might be critical for successful experiment.

scholarly journals DCision-making in tumors governs T cell anti-tumor immunity

Oncogene ◽  
2021 ◽  
Author(s):  
Francesca Alfei ◽  
Ping-Chih Ho ◽  
Wan-Lin Lo
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Dendritic Cell ◽  
Cancer Treatment ◽  
Tumor Immunity ◽  
T Cell Activation ◽  
Cell Activation ◽  
Genetic Alterations ◽  
Immune Checkpoint Blockade ◽  
Oncological Treatment

AbstractThe exploitation of T cell-based immunotherapies and immune checkpoint blockade for cancer treatment has dramatically shifted oncological treatment paradigms and broadened the horizons of cancer immunology. Dendritic cells have emerged as the critical tailors of T cell immune responses, which initiate and coordinate anti-tumor immunity. Importantly, genetic alterations in cancer cells, cytokines and chemokines produced by cancer and stromal cells, and the process of tumor microenvironmental regulation can compromise dendritic cell–T cell cross-talk, thereby disrupting anti-tumor T cell responses. This review summarizes how T cell activation is controlled by dendritic cells and how the tumor microenvironment alters dendritic cell properties in the context of the anti-tumor immune cycle. Furthermore, we will highlight therapeutic options for tailoring dendritic cell-mediated decision-making in T cells for cancer treatment.

scholarly journals Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chandra Chiappin Cardoso ◽  
Camila Matiollo ◽  
Carolina Hilgert Jacobsen Pereira ◽  
Janaina Santana Fonseca ◽  
Helder Emmanuel Leite Alves ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Liver Cirrhosis ◽  
Liver Disease ◽  
Dendritic Cell ◽  
Disease Severity ◽  
Advanced Liver Disease ◽  
Hla Dr ◽  
Acute Decompensation ◽  
Cirrhotic Patients ◽  
Classical Monocytes

AbstractLiver cirrhosis is often complicated by an immunological imbalance known as cirrhosis-associated immune dysfunction. This study aimed to investigate disturbances in circulating monocytes and dendritic cells in patients with acute decompensation (AD) of cirrhosis. The sample included 39 adult cirrhotic patients hospitalized for AD, 29 patients with stable cirrhosis (SC), and 30 healthy controls (CTR). Flow cytometry was used to analyze monocyte and dendritic cell subsets in whole blood and quantify cytokines in plasma samples. Cirrhotic groups showed higher frequencies of intermediate monocytes (iMo) than CTR. AD patients had lower percentages of nonclassical monocytes than CTR and SC. Cirrhotic patients had a profound reduction in absolute and relative dendritic cell numbers compared with CTR and showed higher plasmacytoid/classical dendritic cell ratios. Increased plasma levels of IL-6, IL-10, and IL-17A, elevated percentages of CD62L+ monocytes, and reduced HLA-DR expression on classical monocytes (cMo) were also observed in cirrhotic patients. Patients with more advanced liver disease showed increased cMo and reduced tissue macrophages (TiMas) frequencies. It was found that cMo percentages greater than 90.0% within the monocyte compartment and iMo and TiMas percentages lower than 5.7% and 8.6%, respectively, were associated with increased 90-day mortality. Monocytes and dendritic cells are deeply altered in cirrhotic patients, and subset profiles differ between stable and advanced liver disease. High cMo and low TiMas frequencies may be useful biomarkers of disease severity and mortality in liver cirrhosis.

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