Short Communication: Elevated Labile Iron Levels in CD4 and CD8 T Cells from HIV-Positive Individuals with Undetectable Viral Load

2020 ◽  
Vol 36 (7) ◽  
pp. 597-600
Author(s):  
Radoslava Emilova ◽  
Victor Manolov ◽  
Yana Todorova ◽  
Nina Yancheva ◽  
Ivailo Alexiev ◽  
...  
Keyword(s):  
T Cells ◽  
Viral Load ◽  
Cd8 T Cells ◽  
Short Communication ◽  
Undetectable Viral Load ◽  
Hiv Positive ◽  
Labile Iron

scholarly journals Comparative Evaluation of the Levels of CD4+ and CD8+ T-Cells, Viral Load, and Some Immunomodulatory Trace Elements in ART and ART-naïve HIV Patients in Port-Harcourt, Nigeria

2021 ◽  
pp. 127-133
Author(s):  
Ugochukwu Chioma ◽  
Helen Anthony Waribo ◽  
Donatus O. Onwuli
Keyword(s):  
T Cells ◽  
Trace Elements ◽  
Viral Load ◽  
T Cell ◽  
Cd8 T Cells ◽  
Venous Blood ◽  
Cd4 T Cell ◽  
Hiv Positive ◽  
Port Harcourt ◽  
The Mean

Aim: To evaluate the levels of CD4+ and CD8+ T-cells, viral load, and some immunomodulatory trace elements in ART and ART-naïve HIV patients in Port-Harcourt, Nigeria. Methodology: A total of 150 subjects (males and females) between the ages of 20 and 79 were recruited for the study, out of which 50 subjects were apparently healthy (those who tested negative for HIV), and were used as the control group, while the remaining 100 subjects were those who tested positive for HIV, and were used as the test group; out of this 100 subjects, 70 subjects were on anti-retroviral therapy (ART), while the remaining 30 subjects were not on anti-retroviral therapy (ART naïve). About 13 mls of venous blood was collected from the antecubital fossa of each subject. 3mls was dispensed into an EDTA-anticoagulant bottle, and used for the estimation of CD4+ and CD8+ counts using a BD fluorescent activated cell sorter count (FACSC count) automation. Also, 5mls of the venous blood was dispensed into another EDTA-anticoagulated bottle; it was spun to obtain the plasma which was used to analyze the viral load using real time polymerase chain reaction (RT-PCR) COBAS TaqMan 48 Analyzer. Then, another 5 mls of the venous blood was dispensed into lithium heparin bottle; it was spun to obtain the plasma, which was used for the analysis of copper, iron, zinc and magnesium by colorimetric method using semi auto-analyzer WP 21E, and selenium using atomic absorption spectrophotometer with graphite furnace technique SN-SG 710690. Results: The results showed that there was no significant difference (p>0.05) in the mean levels of CD8+ T-cell, iron and magnesium between the HIV-positive subjects (ART HIV-positive and ART-naïve HIV-positive) and the control. However, the mean levels of CD4+ T-cell and plasma copper were significantly lower (p<0.05) in the HIV-positive subjects compared to the control; also, the mean levels of the CD4+ T-cell in the ART-naïve subjects were significantly lower compared to the ART subjects. The viral load in ART-naïve subjects were significantly higher compared to the ART subjects and control. However, the mean levels of zinc and selenium were significantly lower in the HIV-positive subjects compared to the control. Conclusion: Based on these results, it may be stated that some immunomodulatory trace elements such as zinc and selenium were deficient in HIV-positive subjects, and as such, addition of zinc and/or selenium supplements in the treatment regimen for HIV-positive subjects may be helpful in boosting their immunity and effective management.

scholarly journals CD8 T cells from HIV positive individuals on ART have a skewed differentiation phenotype and impaired proliferative responses

2017 ◽  
Vol 3 ◽  
pp. 35
Author(s):  
G. Clutton ◽  
N. Goonetilleke ◽  
O. Council ◽  
Y. Xu ◽  
J. Warren ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Hiv Positive

scholarly journals P86 An audit of time taken to reach undetectable viral load in therapy-naïve HIV-positive patients initiating art

2015 ◽  
Vol 91 (Suppl 1) ◽  
pp. A44.1-A44
Author(s):  
Muhammad Ismail ◽  
Emmanuel Okpo ◽  
Steve Baguley ◽  
Ambreen Butt ◽  
Daniela Brawley ◽  
...  
Keyword(s):  
Viral Load ◽  
Undetectable Viral Load ◽  
Hiv Positive

scholarly journals Downregulation of CD94/NKG2A inhibitory receptors on CD8+ T cells in HIV infection is more pronounced in subjects with detected viral load than in their aviraemic counterparts

Retrovirology ◽  
2007 ◽  
Vol 4 (1) ◽  
pp. 72 ◽  
Author(s):  
Mustapha Zeddou ◽  
Souad Rahmouni ◽  
Arnaud Vandamme ◽  
Nathalie Jacobs ◽  
Frédéric Frippiat ◽  
...  
Keyword(s):  
T Cells ◽  
Viral Load ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
Inhibitory Receptors

scholarly journals High Rate of Non-detectable HIV-1 RNA among Antiretroviral Drug Naive HIV Positive Individuals in Nigeria

2013 ◽  
Vol 4 ◽  
pp. VRT.S12677 ◽  
Author(s):  
Georgina N. Odaibo ◽  
Isaac F. Adewole ◽  
David O. Olaleye
Keyword(s):  
Viral Load ◽  
Undetectable Viral Load ◽  
Antiretroviral Drugs ◽  
High Rate ◽  
Baseline Viral Load ◽  
Hiv Positive ◽  
Detectable Viral Load ◽  
Cell Counts ◽  
Drug Naïve ◽  
Hiv 1

Plasma HIV-1 RNA concentration, or viral load, is an indication of the magnitude of virus replication and largely correlates with disease progression in an infected person. It is a very useful guide for initiation of therapy and monitoring of response to antiretroviral drugs. Although the majority of patients who are not on antiretroviral therapy (ART) have a high viral load, a small proportion of ART naive patients are known to maintain low levels or even undetectable viral load levels. In this study, we determined the rate of undetectable HIV-1 RNA among ART naive HIV positive patients who presented for treatment at the University College Hospital (UCH), Ibadan, Nigeria from 2005 to 2011. Baseline viral load and CD4 lymphocyte cell counts of 14,662 HIV positive drug naive individuals were determined using the Roche Amplicor version 1.5 and Partec easy count kit, respectively. The detection limits of the viral load assay are 400 copies/mL and 750,000 copies/mL for lower and upper levels, respectively. A total of 1,399 of the 14,662 (9.5%) HIV-1 positive drug naive individuals had undetectable viral load during the study period. In addition, the rate of non-detectable viral load increased over the years. The mean CD4 counts among HIV-1 infected individuals with detectable viral load (266 cells/μL; range = 1 to 2,699 cells/μL) was lower than in patients with undetectable viral load (557 cells/μL; range = 1 to 3,102 cells/μL). About 10% of HIV-1 infected persons in our study population had undetectable viral load using the Roche Amplicor version 1.5.

Association between atazanavir plasma levels and renal function in HIV-positive individuals on antiretroviral therapy with undetectable viral load

2013 ◽  
Vol 41 (5) ◽  
pp. 497-498
Author(s):  
Ana Júlia Luz ◽  
Júlia Poeta ◽  
Rafael Linden ◽  
Marina Venzon Antunes ◽  
Luiza Isola Caminha ◽  
...  
Keyword(s):  
Renal Function ◽  
Viral Load ◽  
Antiretroviral Therapy ◽  
Plasma Levels ◽  
Undetectable Viral Load ◽  
Hiv Positive

scholarly journals A Retrospective Study of the Management of HIV, Hepatitis B and Hepatitis C-Positive Pregnancies in Edinburgh, UK from 1997-2002.

2004 ◽  
Vol 7 (2) ◽  
Author(s):  
Wendy Russell
Keyword(s):  
Viral Load ◽  
Management Practices ◽  
Tracking System ◽  
Undetectable Viral Load ◽  
Hiv Positive ◽  
Hiv Therapy ◽  
Hiv Positive Women ◽  
Valid Data ◽  
Study Child ◽  
Intrapartum Management

STUDY AIMS: This study aims to examine management practices for HIV-positive, HBV-positive and HCV-positive pregnancies over 1997-2002 in Edinburgh, UK, and the effects the diseases have on pregnancy outcomes. RESULTS: Equally for HIV, HBV, and HCV, 50% of the diagnoses were made before pregnancy while the other 50% were detected and diagnosed through antenatal testing. Of the 17 HBV-positive pregnancies 31.6% of the women were highly infectious at delivery and 57.9% were carriers with low infectivity. Of the 17 HIV-positive pregnancies 47.1% of the women had an undetectable viral load and 17.6% were unrecorded at delivery. All 17 HIV-positive pregnancies received ART in varying regimes, 15 (88.2%) were on combination therapy, one delivered vaginally and no women breastfed. All neonates of HBV-positive mothers received immunoglobulin and vaccination and were then breastfed. There were no specific interventions for HCV. Only one study child out of the 38 pregnancies became infected, and this was with HIV. CONCLUSION: Routine screening identifies women with no obvious risk factors, and interventions are largely accepted and effective at reducing vertical transmission. HIV therapy is individually tailored and increasingly uses several agents. Moreover, there is a movement towards allowing low viral load HIV-positive women to deliver vaginally. There are no interventions recommended for HCV infectivity alone. The difficulty collecting information illustrates that no adequate tracking system of infected pregnant women exists. Recommended is the creation of a formal database that includes standardized information such as the viral load of HIV or HCV at delivery, so that outcomes of intrapartum management can be more effectively assessed. No comment can be made on virus-related pregnancy complications, as study numbers are too small for statistically valid data.

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