Long-Term Preservation at Room Temperature of Freeze-Dried Human Tumor Samples Dedicated to Nucleic Acids Analyses

2008 ◽  
Vol 6 (3) ◽  
pp. 191-198 ◽  
Author(s):  
Christophe Leboeuf ◽  
Philippe Ratajczak ◽  
Wei-Li Zhao ◽  
Louis François Plassa ◽  
Magali Court ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Room Temperature ◽  
Human Tumor ◽  
Freeze Dried ◽  
Long Term Preservation

scholarly journals Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

2018 ◽  
Author(s):  
Daniel Clarke ◽  
Adi Idris ◽  
Nigel AJ McMillan
Keyword(s):  
Particle Size ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
Room Temperature ◽  
Sirna Delivery ◽  
Long Term Stability ◽  
Freeze Dried ◽  
Lipid Particles ◽  
End Products

ABSTRACTLiposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage room temperature (RT), 4°C or - 20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.

Long-Term Preservation of Egg and Tissue Homogenates for Determination of Organochlorine Compounds: Freezing Versus Freeze-Drying

1985 ◽  
Vol 68 (1) ◽  
pp. 129-135
Author(s):  
Ross J Norstrom ◽  
Henry T Won
Keyword(s):  
Freeze Drying ◽  
Room Temperature ◽  
Whole Body ◽  
Chromatographic Retention ◽  
Herring Gull ◽  
Vapor Pressures ◽  
Freeze Dried ◽  
Tissue Homogenates ◽  
Long Term Preservation

Abstract Storage of wet egg homogenates at temperatures from —18° to — 28°C was more suitable for long-term preservation than freeze-drying. Changes in residue levels of heptachlor epoxide, oxychlordane, dieldrin, hexachlorobenzene, p,p'-DDE, mirex, and PCBs were not significant over a 3-year period in fresh herring gull egg homogenates stored at —18° to -28°C. Compounds with gas chromatographic retention times shorter than hexachlorobenzene vaporized during freeze-drying at a rate proportional to their volatility. Evaporative losses of components with vapor pressures less than hexachlorobenzene did not occur in naturally contaminated herring gull eggs after storage at room temperature for up to 1 year. Higher losses of all compounds, up to 25% for p,p'-DDE, occurred in freeze-dried whole-body herring gull homogenates. Easily dehydrochlorinated compounds were rapidly degraded in freeze-dried chicken egg homogenate at room temperature: The half-life of p,p'- DDT and p,p'-DDD was about 20 days, and that of α and γ-hexachlorocyclohexane was <16 days. About one-third of oxychlordane in herring gull eggs was lost in 1 year under these conditions, but none was lost after freeze-drying when the homogenate was stored at -18° to -28°C.

scholarly journals Long-term preservation of freeze-dried rabbit sperm by adding rosmarinic acid and different chelating agents

Cryobiology ◽  
2018 ◽  
Vol 81 ◽  
pp. 174-177 ◽  
Author(s):  
Paula Domingo ◽  
Maite Olaciregui ◽  
Noelia González ◽  
Ignacio De Blas ◽  
Lydia Gil
Keyword(s):  
Rosmarinic Acid ◽  
Chelating Agents ◽  
Freeze Dried ◽  
Long Term Preservation

scholarly journals Possibility of Long-Term Preservation of Freeze-Dried Mouse Spermatozoa1

2005 ◽  
Vol 72 (3) ◽  
pp. 568-573 ◽  
Author(s):  
Yosuke Kawase ◽  
Hiroshi Araya ◽  
Nobuo Kamada ◽  
Kou-ichi Jishage ◽  
Hiroshi Suzuki
Keyword(s):  
Freeze Dried ◽  
Long Term Preservation

scholarly journals Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification

2017 ◽  
Vol 66 (1-2) ◽  
pp. 44-50
Author(s):  
Tatjana Vujović ◽  
Đurđina Ružić ◽  
Radosav Cerović
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Shoot Tips ◽  
Lower Survival Rate ◽  
Droplet Vitrification ◽  
Vitrification Solution ◽  
Long Term Preservation ◽  
Direct Immersion

SummaryIn vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material.

scholarly journals Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yuko Kamada ◽  
Sayaka Wakayama ◽  
Ikue Shibasaki ◽  
Daiyu Ito ◽  
Satoshi Kamimura ◽  
...  
Keyword(s):  
Room Temperature ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Term Storage

scholarly journals Urinary nucleic acids can be concentrated, dried on membranes and stored at room temperature in vacuum bags

2014 ◽  
Author(s):  
Fanshuang Zhang ◽  
Yuan Yuan ◽  
Jianqiang Wu ◽  
Youhe Gao
Keyword(s):  
Nucleic Acids ◽  
Large Scale ◽  
Room Temperature ◽  
Invasive Disease ◽  
The Body ◽  
Clinical Samples ◽  
Good Source ◽  
Disease Biomarkers ◽  
Non Invasive

Urine accumulates traces of changes that occur in the body and can potentially serve as a better biomarker source. Urinary nucleic acids are a promising class of non-invasive disease biomarkers. However, long-term frozen human urine samples are not a good source for the extraction of nucleic acids. In this paper, we demonstrate that urinary nucleic acids can be concentrated, dried on membranes and stored in vacuum bags at room temperature for several months. The amount of total RNA on the membranes after storage at room temperature for three months was unchanged. The levels of miR-16 and miR-21 exhibited no significant differences (P = 0.564, 0.386). This simple and economical method makes the large-scale storage of clinical samples of urinary nucleic acids possible.

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