Improved Cellular Immune Response Elicited by a Ubiquitin-Fused DNA Vaccine AgainstMycobacterium tuberculosis

We previously reported that the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15, fused through linkingEimeria acervulina3-1E encoding gene and mature chicken IL-15 (mChIL-15) gene with four flexible amino acid SPGS, could significantly offer protection against homologous challenge. In the present study, the induction of cellular immune response induced by the chimeric DNA vaccine pcDNA-3-1E-linker-mChIL-15 was investigated. Spleen lymphocyte subpopulations were characterized by flow cytometric analysis. The spleen lymphocyte proliferation assays were measured by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide (MTT) method. The mRNA profiles of ChIL-2 and ChIFN-γ in spleen were characterized by means of real-time PCR. Chickens immunized with pcDNA-3-1E-linker-mChIL-15 exhibited significant upregulated level of ChIL-2 and ChIFN-γ transcripts in spleen following two immunizations compared with chickens in other groups (P<0.01). In comparison with pcDNA3.1-immunized and control groups, lymphocyte proliferation, percentage of CD8α+cell, and levels of ChIL-2 and ChIFN-γ transcripts in the group immunized with pcDNA-3-1E-linker-mChIL-15 were significantly increased on day 6 following challenge (P<0.05,P<0.01, andP<0.01, resp.). Our data suggested that the fusion antigen 3-1E-linker-mChIL-15 could be a potential candidate forE. acervulinavaccine development.

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Experimental Leishmania major Infection Suppresses HIV-1 DNA Vaccine Induced Cellular Immune Response

Cells Tissues Organs ◽

10.1159/000079992 ◽

2004 ◽

Vol 177 (3) ◽

pp. 185-188 ◽

Cited By ~ 2

Author(s):

Tara M. Robinson ◽

Robin Nelson ◽

David Artis ◽

Phillip Scott ◽

Jean D. Boyer

Keyword(s):

Immune Response ◽

Dna Vaccine ◽

Cellular Immune Response ◽

Leishmania Major ◽

Cellular Immune ◽

Hiv 1

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A novel adjuvant, the general opioid antagonist naloxone, elicits a robust cellular immune response for a DNA vaccine

International Immunology ◽

10.1093/intimm/dxn139 ◽

2009 ◽

Vol 21 (3) ◽

pp. 217-225 ◽

Cited By ~ 26

Author(s):

A. Jamali ◽

M. Mahdavi ◽

Z. M. Hassan ◽

F. Sabahi ◽

M. J. Farsani ◽

...

Keyword(s):

Immune Response ◽

Dna Vaccine ◽

Cellular Immune Response ◽

Opioid Antagonist ◽

Cellular Immune

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Abstract 47: Treatment With a DNA Vaccine in Experimentally Trypanosoma cruzi -infected Dogs Modulates Cellular Immune Response

Circulation Research ◽

10.1161/res.119.suppl_1.47 ◽

2016 ◽

Vol 119 (suppl_1) ◽

Author(s):

Minerva Arce-Fonseca ◽

Ana C Carbajal-Hernández ◽

Mónica Lozano-Camacho ◽

Silvia C Carrillo-Sánchez ◽

Martha Rios-Castro ◽

...

Keyword(s):

Immune Response ◽

Latin America ◽

Dna Vaccine ◽

Cellular Immune Response ◽

Stimulation Index ◽

Tnf Alpha ◽

Th2 Response ◽

Gene Encoding ◽

Ifn Gamma ◽

Cellular Immune

Chagas Disease (CD) is caused by the protozoan Trypanosoma cruzi . Eight million people are infected in Latin America and 10,000 die annually; because CD has prolonged chronicity is considered the parasitic disease with greater economic burden in Latin America. The 30% to 40% of patients develop chronic chagasic cardiomyopathies and disorders of the gastrointestinal tract. It has not found an effective treatment to cure the chronic disease; nifurtimox and benznidazole in the acute stage offer controversial results. The objective of this study was to test a treatment with plasmid DNA containing T. cruzi genes in dogs with experimental CD and to determine the cellular immune response. Thirty Beagle dogs were divided into 6 groups with 5 dogs each. Dogs from five groups were intraperitoneally infected with 3500 metacyclic trypomastigotes / kg body weight. Two weeks after infection, intramuscular therapeutic vaccinations were administered thrice at 2-week intervals. The plasmids used were: pBCSSP4 (containing a gene encoding an amastigote-specific protein), pBCSP (containing a gene encoding a trans -sialidase protein), pBCSSP4 + pBCSP and pBK-CMV (empty vector); control groups were saline solution mock- treated and non-infected/non-treated dogs. IL-1, IL-6, IL-12, IFN-gamma and TNF-alpha levels were determined by ELISA in each of the serum samples at different times. The proliferative response of spleen cells in vitro at 15 and 30 days after last treatment was studied. IL-6 and IL-12 levels were slightly increased in pBCSSP4 plasmid-treated animals; TNF-alpha and IFN-gamma levels rose after pBCSP plasmid treatment. Treatment with either two recombinant plasmids produced no increase in IL-1 levels. The use of mixed plasmid did not have a synergistic effect. Cell proliferation was induced by DNA plasmid treatment. The highest stimulation index (2.5) was observed in pBCSSP4 plasmid-treated dogs. In conclusion, cellular immune responses are stimulated by therapeutic DNA vaccine; pBCSP plasmid treatment showed polarized type Th1 immune response, while there was a balance in Th1 / Th2 response when pBCSSP4 plasmid was used as treatment. These results support the promising novel therapeutic application with DNA using TcSSP4 and TcSP genes against CD.

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