Prevalence of 16S rRNA Methylase Gene rmtB Among Escherichia coli Isolated from Bovine Mastitis in Ningxia, China

2015 ◽  
Vol 12 (9) ◽  
pp. 770-777 ◽  
Author(s):  
Ting Yu ◽  
Tao He ◽  
Hong Yao ◽  
Jin-Bao Zhang ◽  
Xiao-Na Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Bovine Mastitis ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Kayo Shimada ◽  
Shovita Shrestha ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Clinical Isolates ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following bla NDMs: bla NDM-1, bla NDM-3, bla NDM-4, bla NDM-5, bla NDM-7, bla NDM-12, and bla NDM-13. Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

scholarly journals Characteristics of 32 Acinetobacter baumanni isolates co-harboring OXA-Carbapenemases and 16S rRNA methylase gene

2017 ◽  
Vol 11 (4) ◽  
pp. 110-116
Author(s):  
John Roberts Padde ◽  
Suleiman Hassan Jama ◽  
Li Xiaohan ◽  
Li Mingcheng
Keyword(s):  
16S Rrna ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Outbreak Caused by NDM-1- and RmtB-Producing Escherichia coli in Bulgaria

2014 ◽  
Vol 58 (4) ◽  
pp. 2472-2474 ◽  
Author(s):  
Laurent Poirel ◽  
Encho Savov ◽  
Arzu Nazli ◽  
Angelina Trifonova ◽  
Iva Todorova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
The World ◽  
High Level ◽  
16S Rrna Methylase ◽  
Level Resistance

ABSTRACTTwelve consecutive carbapenem-resistantEscherichia coliisolates were recovered from patients (infection or colonization) hospitalized between March and September 2012 in different units at a hospital in Bulgaria. They all produced the carbapenemase NDM-1 and the extended-spectrum-β-lactamase CTX-M-15, together with the 16S rRNA methylase RmtB, conferring high-level resistance to all aminoglycosides. All those isolates were clonally related and belonged to the same sequence type, ST101. In addition to being the first to identify NDM-producing isolates in Bulgaria, this is the very first study reporting an outbreak of NDM-1-producingE. coliin the world.

Emergence of 16S rRNA methylase gene armA and cocarriage of blaIMP-1 in Pseudomonas aeruginosa isolates from South Korea

2010 ◽  
Vol 68 (4) ◽  
pp. 468-470 ◽  
Author(s):  
Mamata Gurung ◽  
Dong Chan Moon ◽  
Migma Dorji Tamang ◽  
Jungmin Kim ◽  
Yoo Chul Lee ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
South Korea ◽  
Methylase Gene ◽  
16S Rrna Methylase

scholarly journals Cooccurrence of NDM-1, ESBL, RmtC, AAC(6′)-Ib, and QnrB in Clonally Related Klebsiella pneumoniae Isolates Together with Coexistence of CMY-4 and AAC(6′)-Ib in Enterobacter cloacae Isolates from Saudi Arabia

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Mohamed H. Al-Agamy ◽  
Taghrid S. El-Mahdy ◽  
Hesham H. Radwan ◽  
Laurent Poirel
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
High Resistance ◽  
Enterobacter Cloacae ◽  
Sequence Type ◽  
Quinolone Resistance ◽  
Synergy Test ◽  
16S Rrna Methylase

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two Enterobacter cloacae. All isolates except one K. pneumoniae were resistant to tigecycline, but only one K. pneumoniae was resistant to colistin. All produced a carbapenemase according to the Carba NP test, and all were positive for the EDTA-disk synergy test for detection of MBL. Using PCR followed by sequencing, the four K. pneumoniae isolates produced the carbapenemase NDM-1, while the two E. cloacae isolates produced the carbapenemase VIM-1. Genotyping analysis by Multilocus Sequence Typing (MLST) showed that three out of the four K. pneumoniae isolates were clonally related. They had been recovered from the same hospital and belonged to Sequence Type (ST) ST152. In contrast, the fourth K. pneumoniae isolate belonged to ST572. Noticeably, the NDM-1-producing K. pneumoniae additionally produced an extended-spectrum ß-lactamase (ESBL) of the CTX-M type, together with OXA-1 and TEM-1. Surprisingly, the three clonally related isolates produced different CTX-M variants, namely, CTX-M-3, CTX-M-57, and CTX-M-82, and coproduced QnrB, which confers quinolone resistance, and the 16S rRNA methylase RmtC, which confers high resistance to all aminoglycosides. The AAC(6′)-Ib acetyltransferase was detected in both K. pneumoniae and E. cloacae. Mating-out assays using Escherichia coli as recipient were successful for all isolates. The blaNDM-1 gene was always identified on a 70-kb plasmid, whereas the blaVIM-1 gene was located on either a 60-kb or a 150-kb plasmid the two E. cloacae isolates, respectively. To the best of our knowledge, this is the first report of the coexistence of an MBL (NDM-1), an ESBL (CTX-M), a 16S rRNA methylase (RmtC), an acetyltransferase (AAC[6′]-Ib), and a quinolone resistance enzyme (QnrB) in K. pneumoniae isolates recovered from different patients during an outbreak in a Saudi Arabian hospital.

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