Polymorphisms in the Uncoupling Protein 2 Gene Are Associated with Diabetic Retinopathy in Han Chinese Patients with Type 2 Diabetes

Abstract Background: Progranulin (PGRN), a novel pro-inflammatory adipokine, was reported to be related to the development and progression of diabetic retinopathy(DR). However, recently PGRN was established as a renal function-dependent protein, but no data regarding PGRN and DR excluded the influence of decreased renal elimination. This study aimed to investigate the correlation between serum PGRN and DR excluding the effect of deteriorating renal function.Methods: 338 subjects with estimated glomerular filtration rate (eGFR) ≥ 60 ml/min/1.73m2 were divided into four groups of normal controls (NC)(n=76), simple diabetes mellitus (SDM)(n=195) , nonproliferative diabetic retinopathy (NPDR)(n=41), and proliferative diabetic retinopathy (PDR)(n=26). Serum PGRN was quantified by enzyme-linked immunosorbent assay, and further analyses of serum PGRN in different groups were conducted. Results: There was no significant difference of serum PGRN between NC, SDM, NPDR, and PDR groups (P>0.05). Serum PGRN in all subjects negatively and significantly correlated with eGFR (r=-0.144, P<0.05), triglycerides and glucose (TyG) index (r=-0.127, P<0.05), and triglyceride (TG) (r=-0.132, P<0.05), while positively and significantly correlated with low-density lipoprotein cholesterol (LDL-C) (r=0.140, P<0.05). Multivariate stepwise regression analysis indicated only LDL-C (β=1.030, P<0.05) was independently associated with serum PGRN. Conclusions: We demonstrated that serum PGRN levels did not correlate with severity of DR in Chinese patients with type 2 diabetes and eGFR ≥ 60ml/min/1.73m2, but positively and independently correlated with LDL-C.

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Association of 45-bp ins/del polymorphism of uncoupling protein 2 (UCP2) and susceptibility to nonalcoholic fatty liver and type 2 diabetes mellitus in North-west of Iran

10.21203/rs.3.rs-39402/v3 ◽

2020 ◽

Author(s):

Salehe Rezapour ◽

Shiva Ahdi Khosroshahi ◽

Hadi Farajnia ◽

Fatemeh Mohseni ◽

Manouchehr Khoshbaten ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Fatty Liver ◽

Uncoupling Protein ◽

P Value ◽

Uncoupling Protein 2 ◽

Genotype Distribution ◽

Healthy Controls

Abstract Objective: Uncoupling protein 2 (UCP2) plays a crucial role in energy homeostasis via regulation of insulin secretion, free fatty acid concentrations, and lipid metabolism. This study aimed to investigate the association of 45-bp ins/del polymorphism of UCP2 with susceptibility to NAFLD (Non Alcoholic Fatty Liver Disease) and T2DM (Type 2 Diabetes Mellitus). DNA was extracted from the white blood cells of the subjects, and the gene polymorphism was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In this study, 72 patients with NAFLD, 71 healthy individuals as control, 80 patients with T2DM, and 77 healthy controls were enrolled in the study.Results: A higher prevalence of insertion/insertion genotype was observed in T2DM patients compared to the controls (p- value˂ 0.05). But, there was no difference in genotype distribution between NAFLD patients and controls (p-value> 0.05). NAFLD patients with D/D, D/I genotype had higher triglyceride, ALT, and AST levels; however, their HDL levels were lower than healthy controls. Patients with T2DM with D/D or D/I genotype also had significantly higher fasting serum glucose (FSG). While we found an association between the 45bp I/D polymorphism in 3ʹUTR of UCP2 and T2DM, no any correlation between this polymorphism and NAFLD was identified.

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