Glial Cell Line–Derived Neurotrophic Factor Gene Transfer Exerts Protective Effect on Axons in Sciatic Nerve Following Constriction-Induced Peripheral Nerve Injury

2011 ◽  
Vol 22 (6) ◽  
pp. 721-731 ◽  
Author(s):  
Jhih-Yin Shi ◽  
Guei-Sheung Liu ◽  
Li-Feng Liu ◽  
Shiao-Mei Kuo ◽  
Cha-Hwa Ton ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Protective Effect ◽  
Cell Line ◽  
Glial Cell ◽  
Nerve Injury ◽  
Neurotrophic Factor ◽  
Peripheral Nerve Injury ◽  
Factor Gene

scholarly journals The differential effects of pathway- versus target-derived glial cell line–derived neurotrophic factor on peripheral nerve regeneration

2010 ◽  
Vol 113 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Christina K. Magill ◽  
Amy M. Moore ◽  
Ying Yan ◽  
Alice Y. Tong ◽  
Matthew R. MacEwan ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Nerve Fibers ◽  
Sciatic Nerve Crush ◽  
Fiber Density ◽  
Nerve Crush ◽  
Differential Effects

Object Glial cell line–derived neurotrophic factor (GDNF) has potent survival effects on central and peripheral nerve populations. The authors examined the differential effects of GDNF following either a sciatic nerve crush injury in mice that overexpressed GDNF in the central or peripheral nervous systems (glial fibrillary acidic protein [GFAP]–GDNF) or in the muscle target (Myo-GDNF). Methods Adult mice (GFAP-GDNF, Myo-GDNF, or wild-type [WT] animals) underwent sciatic nerve crush and were evaluated using histomorphometry and muscle force and power testing. Uninjured WT animals served as controls. Results In the sciatic nerve crush, the Myo-GDNF mice demonstrated a higher number of nerve fibers, fiber density, and nerve percentage (p < 0.05) at 2 weeks. The early regenerative response did not result in superlative functional recovery. At 3 weeks, GFAP-GDNF animals exhibit fewer nerve fibers, decreased fiber width, and decreased nerve percentage compared with WT and Myo-GDNF mice (p < 0.05). By 6 weeks, there were no significant differences between groups. Conclusions Peripheral delivery of GDNF resulted in earlier regeneration following sciatic nerve crush injuries than that with central GDNF delivery. Treatment with neurotrophic factors such as GDNF may offer new possibilities for the treatment of peripheral nerve injury.

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