Genome-wide CRISPR screen identifies Puf60 as a novel stemness gene of mouse Embryonic Stem Cells

2022 ◽  
Author(s):  
Yue Zhang ◽  
Jiaqi Wang ◽  
Yan Ruan ◽  
Yi Yang ◽  
Yuda Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genome Wide ◽  
Crispr Screen

scholarly journals A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation

2011 ◽  
Vol 195 (6) ◽  
pp. i9-i9 ◽  
Author(s):  
Bart A. Westerman ◽  
A. Koen Braat ◽  
Nicole Taub ◽  
Marko Potman ◽  
Joseph H.A. Vissers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Rnai Screen ◽  
Genome Wide ◽  
A Genome

scholarly journals NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Susan L. Kloet ◽  
Ino D. Karemaker ◽  
Lisa van Voorthuijsen ◽  
Rik G. H. Lindeboom ◽  
Marijke P. Baltissen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Interacting Protein ◽  
Genome Wide

scholarly journals Genome-Wide Identification of Targets and Function of Individual MicroRNAs in Mouse Embryonic Stem Cells

PLoS Genetics ◽  
2010 ◽  
Vol 6 (10) ◽  
pp. e1001163 ◽  
Author(s):  
Sophie A. Hanina ◽  
William Mifsud ◽  
Thomas A. Down ◽  
Katsuhiko Hayashi ◽  
Dónal O'Carroll ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genome Wide ◽  
And Function

scholarly journals Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

2007 ◽  
Vol 12 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Kohta Ikegami ◽  
Misa Iwatani ◽  
Masako Suzuki ◽  
Makoto Tachibana ◽  
Yoichi Shinkai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Deficient Mouse ◽  
Dna Hypomethylation ◽  
Genome Wide

scholarly journals Genome-wide analysis identifies a functional association of Tet1 and Polycomb repressive complex 2 in mouse embryonic stem cells

2013 ◽  
Vol 14 (8) ◽  
pp. R91 ◽  
Author(s):  
Francesco Neri ◽  
Danny Incarnato ◽  
Anna Krepelova ◽  
Stefania Rapelli ◽  
Andrea Pagnani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Polycomb Repressive Complex ◽  
Functional Association ◽  
Polycomb Repressive Complex 2 ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs

2011 ◽  
Vol 18 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Anthony K L Leung ◽  
Amanda G Young ◽  
Arjun Bhutkar ◽  
Grace X Zheng ◽  
Andrew D Bosson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Binding Sites ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genome Wide ◽  
Mature Micrornas

scholarly journals Genome-wide mapping and analysis of active promoters in mouse embryonic stem cells and adult organs

2007 ◽  
Vol 18 (1) ◽  
pp. 46-59 ◽  
Author(s):  
L. O. Barrera ◽  
Z. Li ◽  
A. D. Smith ◽  
K. C. Arden ◽  
W. K. Cavenee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genome Wide

scholarly journals Preservation of Chromatin Organization after Acute Loss of CTCF in Mouse Embryonic Stem Cells

2017 ◽  
Author(s):  
Naoki Kubo ◽  
Haruhiko Ishii ◽  
David Gorkin ◽  
Franz Meitinger ◽  
Xiong Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mammalian Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Chromatin Organization ◽  
Genome Wide ◽  
Binding Factor ◽  
The Impact ◽  
Inactive Chromatin

SummaryThe CCCTC-binding factor (CTCF) is widely regarded as a key player in chromosome organization in mammalian cells, yet direct assessment of the impact of loss of CTCF on genome architecture has been difficult due to its essential role in cell proliferation and early embryogenesis. Here, using auxin-inducible degron techniques to acutely deplete CTCF in mouse embryonic stem cells, we show that cell growth is severely slowed yet chromatin organization remains largely intact after loss of CTCF. Depletion of CTCF reduces interactions between chromatin loop anchors, diminishes occupancy of cohesin complex genome-wide, and slightly weakens topologically associating domain (TAD) structure, but the active and inactive chromatin compartments are maintained and the vast majority of TAD boundaries persist. Furthermore, transcriptional regulation and histone marks associated with enhancers are broadly unchanged upon CTCF depletion. Our results suggest CTCF-independent mechanisms in maintenance of chromatin organization.

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