Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids

In tissue engineering, three-dimensional functional scaffolds with tailored biological properties are needed to be able to mimic the hierarchical structure of biological tissues. Recent developments in additive biomanufacturing allow to extrude multiple materials enabling the fabrication of more sophisticated tissue constructs. These multi-material biomanufacturing systems comprise multiple printing heads through which individual materials are sequentially printed. Nevertheless, as more printing heads are added the fabrication process significantly decreases, since it requires mechanical switching among the physically separated printheads to enable printing multiple materials. In addition, this approach is not able to create biomimetic tissue constructs with property gradients. To address these limitations, this paper presents a novel static mixing extrusion printing head to enable the fabrication of multi-material, functionally graded structures using a single nozzle. Computational fluid dynamics (CFD) was used to numerically analyze the influence of Reynolds number on the flow pattern of biomaterials and mixing efficiency considering different miscible materials.

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Tissue-in-a-Tube: three-dimensional in vitro tissue constructs with integrated multimodal environmental stimulation

Materials Today Bio ◽

10.1016/j.mtbio.2020.100070 ◽

2020 ◽

Vol 7 ◽

pp. 100070

Author(s):

A. Shahin-Shamsabadi ◽

P.R. Selvaganapathy

Keyword(s):

Three Dimensional ◽

Tissue Constructs ◽

Environmental Stimulation

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A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs

SLAS TECHNOLOGY Translating Life Sciences Innovation ◽

10.1177/2472630318775059 ◽

2018 ◽

Vol 23 (6) ◽

pp. 592-598

Author(s):

Brian J. O’Grady ◽

Jason X. Wang ◽

Shannon L. Faley ◽

Daniel A. Balikov ◽

Ethan S. Lippmann ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Viability ◽

Large Scale ◽

Low Cost ◽

Three Dimensional ◽

Tissue Scaffolds ◽

Perfusion System ◽

Pump System ◽

Tissue Constructs ◽

Engineered Tissue

The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.

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Engineering Three-Dimensional Pulmonary Tissue Constructs

Tissue Engineering ◽

10.1089/ten.2005.12.ft-1 ◽

2006 ◽

Vol 0 (0) ◽

pp. 060116055635001

Author(s):

Mark J. Mondrinos ◽

Sirma Koutzaki ◽

Eugean Jiwanmall ◽

Mengyan Li ◽

Jean-Pierre Dechadarevian ◽

...

Keyword(s):

Three Dimensional ◽

Pulmonary Tissue ◽

Tissue Constructs

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An Investigation of Nano-structured Polymers for Use as Bladder Tissue Replacement Constructs

MRS Proceedings ◽

10.1557/proc-711-gg3.4.1 ◽

2001 ◽

Vol 711 ◽

Cited By ~ 3

Author(s):

Anil Thapa ◽

Thomas J. Webster ◽

Karen M. Haberstroh

Keyword(s):

Bladder Wall ◽

Three Dimensional ◽

The Body ◽

Matrix Proteins ◽

Bladder Smooth Muscle ◽

Bladder Tissue ◽

Tissue Replacement ◽

Tissue Constructs

ABSTRACTConventionally, studies investigating the design of synthetic bladder wall substitutes have involved polymers with micro-dimensional structures. Since the body is made up of nano-structured components (e.g., extracellular matrix proteins), our focus has been in the use of nano-structured polymers in order to design a three-dimensional synthetic bladder construct that mimics bladder tissue in vivo. In order to complete this task, we fabricated novel, nano-structured, biodegradable materials to serve as substrates for bladder tissue constructs and tested the cytocompatibility properties of these biomaterials in vitro. The results from our in vitro work to date have provided the first evidence that cellular responses (such as adhesion and proliferation) of bladder smooth muscle cells are enhanced as poly (lactic-co-glycolic acid) (PLGA) surface feature dimensions are reduced into the nanometer range.

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Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation

Scientific Reports ◽

10.1038/srep24474 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 155

Author(s):

Zhengjie Wu ◽

Xin Su ◽

Yuanyuan Xu ◽

Bin Kong ◽

Wei Sun ◽

...

Keyword(s):

Three Dimensional ◽

Tissue Constructs ◽

Controllable Degradation ◽

Dimensional Cell

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Effect of Sodium Alginate Concentration During Laser-Assisted Printing of Alginate Tubes

ASME/ISCIE 2012 International Symposium on Flexible Automation ◽

10.1115/isfa2012-7253 ◽

2012 ◽

Author(s):

Jingyuan Yan ◽

Hemanth Gudapati ◽

Yong Huang ◽

Changxue Xu

Keyword(s):

Sodium Alginate ◽

Three Dimensional ◽

Free Form ◽

Thin Wall ◽

Imaging Analysis ◽

Tissue Constructs ◽

Alginate Concentration ◽

Printing Technique ◽

Resolution Imaging ◽

Tube Structure

For the free-form fabrication of various tissue constructs, three-dimensional (3D) additive printing technology has emerged as a promising approach for organ fabrication. This study aims to print a tube structure using a laser-assisted orifice-free printing technique and further investigate the effect of sodium alginate concentration on the tube wall thickness. Alginate tubes have been successfully printed. It is found that highly viscous materials can be laser printed into well-defined tube structures. A higher concentration solution such as the 8% sodium alginate solution leads to a thin wall, meaning a better resolution. Imaging analysis also illustrates that higher concentration solutions help develop smooth, slim jets upon the incidence of laser pulse.

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