scholarly journals A critical balance between Pax8 and the Hippo mediator TAZ determines sodium-iodide symporter NIS expression and function

Thyroid ◽  
2021 ◽  
Author(s):  
Celia Fernández-Méndez ◽  
Pilar Santisteban
Keyword(s):  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
And Function

scholarly journals Forskolin, 8-Br-3′,5′-Cyclic Adenosine 5′-Monophosphate, and Catalytic Protein Kinase A Expression in the Nucleus Increase Radioiodide Uptake and Sodium/Iodide Symporter Protein Levels in RET/PTC1-Expressing Cells

2004 ◽  
Vol 89 (12) ◽  
pp. 6168-6172 ◽  
Author(s):  
Anjli Venkateswaran ◽  
Derek K. Marsee ◽  
Steven H. Green ◽  
Sissy M. Jhiang
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Thyroid Cells ◽  
Protein Levels ◽  
And Function ◽  
Kinase A

Abstract RET/PTC1, a thyroid-specific oncogene, has been reported to down-regulate sodium/iodide symporter (NIS) expression and function in vitro and in vivo. Recently, RET/PTC1 has been shown to interfere with TSH signaling at multiple levels in thyroid cells. The objective of this study was to investigate whether RET/PTC1-mediated NIS reduction can be rescued by activating cAMP-protein kinase A (PKA) pathways. We showed that both forskolin and 8-Br-cAMP increase radioiodide uptake and NIS protein in RET/PTC1-expressing cells to the same extent as the parental PC Cl 3 cells. We found that RET/PTC1 decreases nuclear localization of catalytic PKA, and forskolin treatment was able to counteract this RET/PTC1 effect. Furthermore, transient expression of catalytic PKA in the nucleus increased radioiodide uptake and NIS protein in RET/PTC1-expressing cells. Taken together, these studies suggest that RET/PTC1 down-regulates NIS expression by interrupting TSH/cAMP signaling, and this RET/PTC1 effect can be reversed by activating cAMP-PKA pathways.

scholarly journals Monoclonal antibodies against the human sodium iodide symporter: utility for immunocytochemistry of thyroid cancer

1999 ◽  
Vol 163 (3) ◽  
pp. 495-504 ◽  
Author(s):  
MR Castro ◽  
ER Bergert ◽  
TG Beito ◽  
PC Roche ◽  
SC Ziesmer ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fusion Protein ◽  
Sodium Iodide ◽  
Cell Cancer ◽  
Thyroid Tissue ◽  
Follicular Epithelium ◽  
Sodium Iodide Symporter ◽  
Major Band ◽  
Iodide Transport ◽  
And Function

The recent cloning of the thyroidal protein that is responsible for iodide transport, the sodium iodide symporter (hNIS), has made possible studies designed to characterize its structure, function and expression in thyroidal tissues. Using a mannose binding protein (MBP)-hNIS fusion protein as antigen, we have developed mouse monoclonal antibodies against hNIS to utilize as tools in such studies. Twenty-four clones were initially recovered which recognized the MBP-hNIS fusion protein, but only two of them were specific for hNIS while the others recognized MBP alone. Both antibodies were found to be immunoglobulin G (IgG) 1kappa (kappa). The specificity of antibodies was tested by Western blotting using membranes prepared from COS-7 cells transiently transfected with the pcDNA3 plasmid containing the full-length hNIS cDNA, or cells transfected with the pcDNA3 vector. A major band with a molecular weight (MW) of approximately 97 kDa, and several minor bands with MW of approximately 160 kDa, approximately 68 kDa, approximately 30 kDa and approximately 15 kDa, were detected specifically in the hNIS-transfected cells. After enzymatic deglycosylation, the major band was present at 68 kDa, as expected based upon the amino acid sequence of hNIS. Immunohistochemistry was performed with several different types of thyroid tissue and non-thyroidal tissues, using the monoclonal antibodies. Strong immunostaining was observed in Graves' tissue, with intermediate staining in papillary and follicular thyroid cancers and an absence of staining in Hurthle cell cancer. The staining was specific for the follicular epithelium and was concentrated in the basolateral portion of the cell membrane. These monoclonal hNIS antibodies should prove useful in the characterization of NIS expression in benign and malignant thyroid tissue and in studies characterizing its structure and function.

scholarly journals Resveratrol Inhibits Sodium/Iodide Symporter Gene Expression and Function in Rat Thyroid Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107936 ◽  
Author(s):  
Cesidio Giuliani ◽  
Ines Bucci ◽  
Serena Di Santo ◽  
Cosmo Rossi ◽  
Antonino Grassadonia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Thyroid Cells ◽  
Rat Thyroid ◽  
And Function

In vivo expression and function of the sodium iodide symporter following gene transfer in the MATLyLu rat model of metastatic prostate cancer

The Prostate ◽  
2002 ◽  
Vol 50 (3) ◽  
pp. 170-178 ◽  
Author(s):  
Krista M.D. La Perle ◽  
Daniel Shen ◽  
Tara L.F. Buckwalter ◽  
Bonnie Williams ◽  
Aaron Haynam ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gene Transfer ◽  
Rat Model ◽  
Metastatic Prostate Cancer ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
In Vivo Expression ◽  
And Function

Cooperation of histone deacetylase inhibitors SAHA and valproic acid in promoting sodium/iodide symporter expression and function in rat Leydig testicular carcinoma cells

Endocrine ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 148-152 ◽  
Author(s):  
V. Maggisano ◽  
C. Puppin ◽  
M. Celano ◽  
M. D’Agostino ◽  
M. Sponziello ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Histone Deacetylase ◽  
Sodium Iodide ◽  
Histone Deacetylase Inhibitors ◽  
Carcinoma Cells ◽  
Sodium Iodide Symporter ◽  
Testicular Carcinoma ◽  
And Function

Imaging of mesenchymal stem cell recruitment into the stroma of hepatic colon cancer metastases using the sodium iodide symporter (NIS)

2013 ◽  
Vol 121 (03) ◽  
Author(s):  
K Knoop ◽  
N Schwenk ◽  
P Dolp ◽  
MJ Willhauck ◽  
C Zischek ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Cell Recruitment ◽  
Cancer Metastases

Identification of novel sodium iodide symporter (NIS) interactors which modulate iodide uptake

2016 ◽  
Author(s):  
Alice Fletcher ◽  
Vikki Poole ◽  
Bhavika Modasia ◽  
Waraporn Imruetaicharoenchoke ◽  
Rebecca Thompson ◽  
...  
Keyword(s):  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake

Identification of novel sodium iodide symporter interactors which modulate iodide uptake

2017 ◽  
Author(s):  
Alice Fletcher ◽  
Vikki Poole ◽  
Bhavika Modasia ◽  
Waraporn Imruetaicharoenchoke ◽  
Rebecca Thompson ◽  
...  
Keyword(s):  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake

An investigation into sodium-iodide symporter (NIS) dimerization and its impact on radioiodide uptake in thyroid cancer

2017 ◽  
Author(s):  
Rebecca J. Thompson ◽  
Alice Fletcher ◽  
Hannah Nieto ◽  
Mohammed Alshahrani ◽  
Katie Baker ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter

Identification of novel sodium iodide symporter (NIS) interactors which modulate radioiodine uptake

2018 ◽  
Author(s):  
Alice Fletcher ◽  
Vikki Poole ◽  
Caitlin Thornton ◽  
Kate Baker ◽  
Rebecca Thompson ◽  
...  
Keyword(s):  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Radioiodine Uptake
Sign in / Sign up

Export Citation Format

Share Document