Marek's Disease Virus–Induced Immunosuppression: Array Analysis of Chicken Immune Response Gene Expression Profiling

2010 ◽  
Vol 23 (3) ◽  
pp. 309-319 ◽  
Author(s):  
Mohammad Heidari ◽  
Aimie J. Sarson ◽  
Marianne Huebner ◽  
Shayan Sharif ◽  
Dmitry Kireev ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Gene Expression Profiling ◽  
Disease Virus ◽  
Expression Profiling ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Immune Response Gene ◽  
Array Analysis ◽  
Response Gene

scholarly journals Protective Effect of Avian Myelomonocytic Growth Factor in Infection with Marek’s Disease Virus

2002 ◽  
Vol 76 (3) ◽  
pp. 1062-1070 ◽  
Author(s):  
Aouatef Djeraba ◽  
Eugène Musset ◽  
John W. Lowenthal ◽  
David B. Boyle ◽  
Anne-Marie Chaussé ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Growth Factor ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
No Production ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Inos Gene

ABSTRACT Marek’s disease virus (MDV) is a herpesvirus that induces T lymphomas in chickens. The aim of this study was to assess the role of the macrophage activator chicken myelomonocytic growth factor (cMGF) in controlling MDV infection. B13/B13 chickens, which are highly susceptible to MD, were either treated with cMGF delivered via a live fowlpox virus (fp/cMGF) or treated with the parent vector (fp/M3) or were left as untreated controls. Seven days later, when challenged with the very virulent RB-1B strain of MDV, the spleens of chickens treated with fp/cMGF showed increased expression of the inducible nitric oxide synthase (iNOS) gene compared to those of control chickens and fp/M3-treated chickens. Increased iNOS gene expression was also accompanied by greater induction of gamma interferon and macrophage inflammatory protein (K203) gene expression, both possible activators of iNOS. fp/cMGF treatment also increased the number of monocytes and systemic NO production in contrast to fp/M3 treatment. Even though cMGF treatment was unable to prevent death for the chickens, it did prolong their survival time, and viremia and tumor incidence were greatly reduced. In addition, cMGF treatment improved the partial protection induced by vaccination with HVT (herpesvirus isolated from turkeys) against RB-1B, preventing 100% mortality (versus 66% with vaccination alone) and greatly reducing tumor development. Treatment with fp/M3 did not have such effects. These results suggest that cMGF may play multiple roles in protection against MD. First, it may enhance the innate immune response by increasing the number and activity of monocytes and macrophages, resulting in increased NO production. Second, it may enhance the acquired immune response, indicated by its ability to enhance vaccine efficacy.

scholarly journals Clinical quantitation of immune signature in follicular lymphoma by RT-PCR–based gene expression profiling

Blood ◽  
2008 ◽  
Vol 111 (9) ◽  
pp. 4764-4770 ◽  
Author(s):  
Richard J. Byers ◽  
Ebrahim Sakhinia ◽  
Preethi Joseph ◽  
Caroline Glennie ◽  
Judith A. Hoyland ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Follicular Lymphoma ◽  
Gene Expression Profiling ◽  
Real Time ◽  
Expression Profiling ◽  
Immune Response Gene ◽  
Routine Practice ◽  
Gene Signatures ◽  
Survival Interval

Abstract Microarray gene expression profiling studies have demonstrated immune response gene signatures that appear predictive of outcome in follicular lymphoma (FL). However, measurement of these marker genes in routine practice remains difficult. We have therefore investigated the immune response in FL using real-time polymerase chain reaction (PCR) to measure expression levels of 35 candidate Indicator genes, selected from microarray studies, to polyA cDNAs prepared from 60 archived human frozen lymph nodes, in parallel with immunohistochemical analysis for CD3, CD4, CD7, CD8, CD10, CD20, CD21, and CD68. High levels of CCR1, a marker of monocyte activation, were associated with a shorter survival interval, and high levels of CD3 with better survival, while immunohistochemistry demonstrated association of high numbers of CD68+ macrophages with a shorter survival interval and of high numbers of CD7+ T cells with a longer survival interval. The results confirm the role of the host immune response in outcome in FL and identify CCR1 as a prognostic indicator and marker of an immune switch between macrophages and a T cell–dominant response. They demonstrate the utility of polyA DNA and real-time PCR for measurement of gene signatures and the applicability of using this type of “molecular block” in clinical practice.

Gene expression profiling in PC12 cells infected with an oncolytic Newcastle disease virus strain

2014 ◽  
Vol 185 ◽  
pp. 10-22 ◽  
Author(s):  
András Balogh ◽  
Judit Bátor ◽  
Lajos Markó ◽  
Mária Németh ◽  
Marianna Pap ◽  
...  
Keyword(s):  
Gene Expression ◽  
Newcastle Disease Virus ◽  
Gene Expression Profiling ◽  
Pc12 Cells ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Expression Profiling ◽  
Newcastle Disease Virus Strain

scholarly journals Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR

2006 ◽  
Vol 12 (7-8) ◽  
pp. 185-195 ◽  
Author(s):  
Kevin McLoughlin ◽  
Ken Turteltaub ◽  
Danute Bankaitis-Davis ◽  
Richard Gerren ◽  
Lisa Siconolfi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Blood Donors ◽  
Dynamic Range ◽  
Immune Response Gene ◽  
Rt Pcr ◽  
Response Gene

Modulation of immune response gene expression by echinacea extracts: results of a gene array analysisThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

2007 ◽  
Vol 85 (11) ◽  
pp. 1091-1098 ◽  
Author(s):  
M. Altamirano-Dimas ◽  
J. B. Hudson ◽  
D. Cochrane ◽  
C. Nelson ◽  
J. T. Arnason
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Gene Array ◽  
Immune Response Gene ◽  
Natural Health Products ◽  
Response Gene ◽  
Cytokines And Chemokines ◽  
Biological Responses ◽  
Health Products ◽  
Factor C

Echinacea extracts have traditionally been used in the treatment of many infectious and other diseases (such as rhinovirus colds), and research has revealed the presence of various bioactivities in these extracts, particularly those connected with immune responses. We examined the effects of Echinacea by using gene expression analysis in a line of human bronchial epithelial cells, with or without rhinovirus infection. More than 13 000 human genes were evaluated. From these analyses we focused primarily on immune response genes and found that both Echinacea extracts, one predominantly rich in polysaccharides and the other rich in alkylamides and caffeic acid derivatives, stimulated the expression of numerous genes. These included a number of cytokines and chemokines, although the pattern of stimulation was different. In addition, Echinacea extracts tended to neutralize the effects of the rhinovirus. When the immune response gene pathways were analyzed with the Ingenuity Pathway program, it became apparent that many of them were interconnected through a major node, the transcription factor C/EBPβ (CAAT/enhancer-binding protein β) and its related C/EBP proteins. This suggests that Echinacea can bring about important biological responses in cells by virtue of interactions between components of the extract and a small number of intracellular factors involved in multiple signaling pathways.

scholarly journals Gene expression profiling of human stenotic aorto-coronary bypass grafts by cDNA array analysis

2003 ◽  
Vol 23 (4) ◽  
pp. 620-625 ◽  
Author(s):  
Michael Hilker ◽  
Tina Längin ◽  
Ulrich Hake ◽  
Franz-Xaver Schmid ◽  
Wlodzimierz Kuroczynski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cdna Array ◽  
Coronary Bypass ◽  
Bypass Grafts ◽  
Array Analysis ◽  
Coronary Bypass Grafts
Sign in / Sign up

Export Citation Format

Share Document