Simulating Mucociliary Transport Using the Method of Regularized Stokeslets

Because of its possible importance in cystic fibrosis (CF) pulmonary pathogenesis, the effect of anion and liquid secretion inhibitors on airway mucociliary transport was examined. When excised porcine tracheas were treated with ACh to induce gland liquid secretion, the rate of mucociliary transport was increased nearly threefold from 2.5 ± 0.5 to 6.8 ± 0.8 mm/min. Pretreatment with both bumetanide and dimethylamiloride (DMA), to respectively inhibit Cl− and HCO[Formula: see text]secretion, significantly reduced mucociliary transport in the presence of ACh by 92%. Pretreatment with the anion channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid similarly reduced mucociliary transport in ACh-treated airways by 97%. These agents did not, however, reduce ciliary beat frequency. Luminal application of benzamil to block liquid absorption significantly attenuated the inhibitory effects of bumetanide and DMA on mucociliary transport. We conclude that anion and liquid secretion is essential for normal mucociliary transport in glandular airways. Because the CF transmembrane conductance regulator protein likely mediates Cl−, HCO[Formula: see text], and liquid secretion in normal glands, we speculate that impairment of gland liquid secretion significantly contributes to defective mucociliary transport in CF.

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Influence of corticosterone on tracheal mucociliary transport in pigeons.

The Japanese Journal of Pharmacology ◽

10.1254/jjp.52.496 ◽

1990 ◽

Vol 52 (3) ◽

pp. 496-499 ◽

Cited By ~ 5

Author(s):

Hirofumi KAI ◽

Satoko YAMAMOTO ◽

Kazuo TAKAHAMA ◽

Takeshi MIYATA

Keyword(s):

Mucociliary Transport

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Correlations among Mucociliary Transport, Ciliary Function, and Ciliary Structure

American Journal of Rhinology ◽

10.2500/105065898782102945 ◽

1998 ◽

Vol 12 (1) ◽

pp. 53-58 ◽

Cited By ~ 20

Author(s):

Mark Jorissen

Keyword(s):

Defense Mechanisms ◽

Ex Vivo ◽

Beat Frequency ◽

Ciliary Beat Frequency ◽

Preliminary Evidence ◽

Relevant Parameter ◽

Mucociliary Transport ◽

Ciliary Dyskinesia ◽

Ciliary Beat

Mucociliary transport is one of the most important defense mechanisms of the airway. Mucociliary transport time or rate, as measured using the saccharin test or the radioisotope technique, respectively, is clinically the most relevant parameter, although subject to large intra- and interindividual variability. There is no correlation between mucociliary transport in vivo and ciliary beat frequency ex vivo. Preliminary evidence demonstrates that mucociliary transport correlates with ciliary structure and orientation as investigated with transmission and scanning electron microscopy. A correlation is presented between ciliary beat frequency and secondary ciliary abnormalities. This correlation can best be described according to the logistic sigmoid model (r = 0.69). Based on these functional data, an ultrastructural distinction is proposed among normal (less than 5%), light (5 to 15%), moderate (15 to 25%), and severe (more than 25%) secondary ciliary dyskinesia.

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Experimental study of the possibility of topical application of polyvinylpyrrolidone for epistaxis

Terapevt (General Physician) ◽

10.33920/med-12-2112-02 ◽

2021 ◽

pp. 15-19

Author(s):

Viktoriya Valentinovna Bykova ◽

Svetlana Aleksandrovna Chubka

Keyword(s):

Experimental Study ◽

Nasal Cavity ◽

Topical Application ◽

Nasal Packing ◽

Recurrent Bleeding ◽

Mucociliary Transport ◽

Prolonged Stay ◽

Bleeding Vessel ◽

Recurrent Epistaxis ◽

Local Fibrinolysis

Nasal packing is widely used in patients with epistaxis, especially in cases where it is impossible to electrocoagulate the bleeding vessel. The disadvantages of gauze packing are well known. First of all, this is the risk of recurrent bleeding after removing the tampons from the nose. The cause of recurrent epistaxis is the activation of local fibrinolysis during prolonged stay of the tampon in the nasal cavity. To overcome this drawback, we have proposed the topical application of polyvinylpyrrolidone (PVP). In an experiment on animals, the absence of a damaging effect of PVP on mucociliary transport was proved.

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Luffa Operculata Affects Mucociliary Function of the Isolated Frog Palate

American Journal of Rhinology ◽

10.1177/194589240501900406 ◽

2005 ◽

Vol 19 (4) ◽

pp. 353-357 ◽

Cited By ~ 2

Author(s):

Mônica Aidar Menon-Miyake ◽

Regiani Carvalho de Oliveira ◽

Geraldo Lorenzi-Filho ◽

Paulo Hilário Nascimento Saldiva ◽

Ossamu Butugan

Keyword(s):

Alternative Medicine ◽

Nasal Mucosa ◽

Beat Frequency ◽

Ciliary Beat Frequency ◽

The United States ◽

Mucociliary Transport ◽

Transport Velocity ◽

Mucociliary Function ◽

Frog Palate ◽

Ciliary Beat

Background Luffa operculata is a medicinal plant used in homeopathic and alternative medicine. In the United States, it is sold in a purified spray form, whereas a homemade L. operculata dry fruit infusion (DFI) is commonly used in Latin America. The L. operculata DFI is applied intranasally, inducing profuse mucous secretion and relieving nasal symptoms. Nevertheless, this medication may cause irritation of the nasal mucosa, as well as epistaxis or anosmia. Given the growing popularity of alternative medicine, a decision was made to evaluate the effects of this substance on mucous membranes. Methods The effects of L. operculata DFI on mucociliary transport velocity, ciliary beat frequency, and transepithelial potential difference (PD) were evaluated in an isolated frog palate preparation. We tested 46 palates immediately before immersion and again at 5 and 20 minutes after immersion. Four groups (n = 10) were tested in frog Ringer: control; L. operculata DFI, 60 mg/L; 600 mg/L; and 1200 mg/L. An additional group was tested using L. operculata DFI prepared with water (600 mg/L of H2O, n = 6). Epithelial samples were harvested for ultrastructural study. Results In treated palates, mucociliary transport velocity and ciliary beat frequency decreased significantly (p < 0.001 and p < 0.008, respectively). There was a dose-dependent decrease in PD modulus (p < .007). Our PD findings indicated ion-fluid transport abnormalities, which were confirmed by transmission electron microscopy that showed enlargement of interepithelial spaces. Conclusion In this ex vivo model, the L. operculata DFI infusion promoted significant changes in the mucociliary function of the epithelium, suggesting that it is potentially noxious to human nasal mucosa.

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