scholarly journals Cdc7-Dbf4 Regulates NDT80 Transcription as Well as Reductional Segregation during Budding Yeast Meiosis

2008 ◽  
Vol 19 (11) ◽  
pp. 4956-4967 ◽  
Author(s):  
Hsiao-Chi Lo ◽  
Lihong Wan ◽  
Adam Rosebrock ◽  
Bruce Futcher ◽  
Nancy M. Hollingsworth
Keyword(s):  
Dna Replication ◽  
Protein Kinase ◽  
Dna Synthesis ◽  
Budding Yeast ◽  
Genetic Approach ◽  
Chemical Genetic ◽  
Meiotic Progression ◽  
Cdc7 Kinase ◽  
Yeast Meiosis ◽  
Meiosis I

In budding yeast, as in other eukaryotes, the Cdc7 protein kinase is important for initiation of DNA synthesis in vegetative cells. In addition, Cdc7 has crucial meiotic functions: it facilitates premeiotic DNA replication, and it is essential for the initiation of recombination. This work uses a chemical genetic approach to demonstrate that Cdc7 kinase has additional roles in meiosis. First, Cdc7 allows expression of NDT80, a meiosis-specific transcriptional activator required for the induction of genes involved in exit from pachytene, meiotic progression, and spore formation. Second, Cdc7 is necessary for recruitment of monopolin to sister kinetochores, and it is necessary for the reductional segregation occurring at meiosis I. The use of the same kinase to regulate several distinct meiosis-specific processes may be important for the coordination of these processes during meiosis.

A meiosis-specific protein kinase, Ime2, is required for the correct timing of DNA replication and for spore formation in yeast meiosis

1996 ◽  
Vol 253 (3) ◽  
pp. 278-288 ◽  
Author(s):  
M. Foiani ◽  
E. Nadjar-Boger ◽  
R. Capone ◽  
S. Sagee ◽  
T. Hashimshoni ◽  
...  
Keyword(s):  
Dna Replication ◽  
Protein Kinase ◽  
Specific Protein ◽  
Spore Formation ◽  
Yeast Meiosis ◽  
Specific Protein Kinase

scholarly journals Protein phosphatase 2A (PP2A) promotes anaphase entry after DNA replication stress in budding yeast

2021 ◽  
Author(s):  
Cory Haluska ◽  
Fengzhi Jin ◽  
Yanchang Wang
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Protein Phosphatase ◽  
Budding Yeast ◽  
Replication Stress ◽  
S Phase ◽  
Viability Loss ◽  
Phosphatase 2A ◽  
Dna Replication Stress

DNA replication stress activates the S-phase checkpoint that arrests the cell cycle, but it is poorly understood how cells recover from this arrest. Cyclin-dependent kinase (CDK) and Protein Phosphatase 2A (PP2A) are key cell cycle regulators, and Cdc55 is a regulatory subunit of PP2A in budding yeast. We found that yeast cells lacking functional PP2ACdc55 showed slow growth in the presence of hydroxyurea (HU), a DNA synthesis inhibitor, without obvious viability loss. Moreover, PP2A mutants exhibited delayed anaphase entry and sustained levels of anaphase inhibitor Pds1 after HU treatment. A DNA damage checkpoint Chk1 phosphorylates and stabilizes Pds1. We showed that chk1Δ and mutation of the Chk1 phosphorylation sites in Pds1 largely restored efficient anaphase entry in PP2A mutants after HU treatment. In addition, deletion of SWE1 that encodes the inhibitory kinase for CDK or mutation of the Swe1 phosphorylation site in CDK ( cdc28F19) also suppressed the anaphase entry delay in PP2A mutants after HU treatment. Our genetic data suggest that Swe1/CDK acts upstream of Pds1. Surprisingly, cdc55Δ showed significant suppression to the viability loss of S-phase checkpoint mutants during DNA synthesis block. Together, our results uncover a PP2A-Swe1-CDK-Chk1-Pds1 axis that promotes recovery from DNA replication stress.

scholarly journals Cdc14 phosphatase directs centrosome re-duplication at the meiosis I to meiosis II transition in budding yeast

2017 ◽  
Vol 2 ◽  
pp. 2 ◽  
Author(s):  
Colette Fox ◽  
Juan Zou ◽  
Juri Rappsilber ◽  
Adele L. Marston
Keyword(s):  
Cell Cycle ◽  
Dna Replication ◽  
Chromosome Segregation ◽  
Budding Yeast ◽  
Spindle Pole Body ◽  
Initial Step ◽  
Mitotic Cell ◽  
Spindle Pole ◽  
Fluorescence And Electron Microscopy ◽  
Meiosis I

Background Gametes are generated through a specialized cell division called meiosis, in which ploidy is reduced by half because two consecutive rounds of chromosome segregation, meiosis I and meiosis II, occur without intervening DNA replication. This contrasts with the mitotic cell cycle where DNA replication and chromosome segregation alternate to maintain the same ploidy. At the end of mitosis, CDKs are inactivated. This low CDK state in late mitosis/G1 allows for critical preparatory events for DNA replication and centrosome/spindle pole body (SPB) duplication. However, their execution is inhibited until S phase, where further preparatory events are also prevented. This “licensing” ensures that both the chromosomes and the centrosomes/SPBs replicate exactly once per cell cycle, thereby maintaining constant ploidy. Crucially, between meiosis I and meiosis II, centrosomes/SPBs must be re-licensed, but DNA re-replication must be avoided. In budding yeast, the Cdc14 protein phosphatase triggers CDK down regulation to promote exit from mitosis. Cdc14 also regulates the meiosis I to meiosis II transition, though its mode of action has remained unclear. Methods Fluorescence and electron microscopy was combined with proteomics to probe SPB duplication in cells with inactive or hyperactive Cdc14. Results We demonstrate that Cdc14 ensures two successive nuclear divisions by re-licensing SPBs at the meiosis I to meiosis II transition. We show that Cdc14 is asymmetrically enriched on a single SPB during anaphase I and provide evidence that this enrichment promotes SPB re-duplication. Cells with impaired Cdc14 activity fail to promote extension of the SPB half-bridge, the initial step in morphogenesis of a new SPB. Conversely, cells with hyper-active Cdc14 duplicate SPBs, but fail to induce their separation. Conclusion Our findings implicate reversal of key CDK-dependent phosphorylations in the differential licensing of cyclical events at the meiosis I to meiosis I transition.

Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae

1982 ◽  
Vol 2 (2) ◽  
pp. 171-178
Author(s):  
M J Clancy ◽  
L M Smith ◽  
P T Magee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzyme Activity ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Developmental Regulation ◽  
Specific Enzyme ◽  
Rapid Degradation ◽  
Specific Enzyme Activity ◽  
Alpha Glucosidase ◽  
Meiosis I

An alpha-glucosidase activity (SAG) occurs in a/alpha Saccharomyces cerevisiae cells beginning at about 8 to 10 h after the initiation of sporulation. This enzyme is responsible for the rapid degradation of intracellular glycogen which follows the completion of meiosis in these cells. SAG differs from similar activities present in vegetative cells and appears to be a sporulation-specific enzyme. Cells arrested at various stages in sporulation (DNA replication, recombination, meiosis I, and meiosis II) were examined for SAG activity; the results show that SAG appearance depends on DNA synthesis and some recombination events but not on the meiotic divisions.

scholarly journals A chemical-genetic approach to elucidate protein kinase function in planta

2007 ◽  
Vol 65 (6) ◽  
pp. 817-827 ◽  
Author(s):  
Maik Böhmer ◽  
Tina Romeis
Keyword(s):  
Protein Kinase ◽  
Genetic Approach ◽  
In Planta ◽  
Chemical Genetic

scholarly journals Developmental regulation of a sporulation-specific enzyme activity in Saccharomyces cerevisiae.

1982 ◽  
Vol 2 (2) ◽  
pp. 171-178 ◽  
Author(s):  
M J Clancy ◽  
L M Smith ◽  
P T Magee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzyme Activity ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Developmental Regulation ◽  
Specific Enzyme ◽  
Rapid Degradation ◽  
Specific Enzyme Activity ◽  
Alpha Glucosidase ◽  
Meiosis I

An alpha-glucosidase activity (SAG) occurs in a/alpha Saccharomyces cerevisiae cells beginning at about 8 to 10 h after the initiation of sporulation. This enzyme is responsible for the rapid degradation of intracellular glycogen which follows the completion of meiosis in these cells. SAG differs from similar activities present in vegetative cells and appears to be a sporulation-specific enzyme. Cells arrested at various stages in sporulation (DNA replication, recombination, meiosis I, and meiosis II) were examined for SAG activity; the results show that SAG appearance depends on DNA synthesis and some recombination events but not on the meiotic divisions.

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