Chlorpyrifos exposure induces lipid metabolism disorder at the physiological and transcriptomic levels in larval zebrafish

Abstract Chlorpyrifos (CPF) is a widely used insecticide in pest control, and it can affect aquatic animals by contaminating the water. In this study, larval zebrafish were exposed to CPF at concentrations of 30, 100 and 300 μg/l for 7 days. In the CPF-treated group, lipid droplet accumulation was reduced in larval zebrafish. The levels of triglyceride (TG), total cholesterol (TC), and pyruvate were also decreased after CPF exposure. Cellular apoptosis were significantly increased in the heart tissue after CPF exposure compared with the control. Transcription changes in cardiovascular genes were also observed. Through transcriptome analysis, we found that the transcription of 465 genes changed significantly, with 398 upregulated and 67 downregulated in the CPF-treated group, indicating that CPF exposure altered the transcription of genes. Among these altered genes, a number of genes were closely related to the glucose and lipid metabolism pathways. Furthermore, we also confirmed that the transcription of genes related to fatty acid synthesis, TC synthesis, and lipogenesis were significantly decreased in larval zebrafish after exposure to CPF. These results indicated that CPF exposure induced lipid metabolism disorders associated with cardiovascular toxicity in larval zebrafish.

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Quercetin Improves Glucose and Lipid Metabolism of Diabetic Rats: Involvement of Akt Signaling and SIRT1

Journal of Diabetes Research ◽

10.1155/2017/3417306 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 28

Author(s):

Jing Peng ◽

Qingde Li ◽

Keye Li ◽

Li Zhu ◽

Xiaoding Lin ◽

...

Keyword(s):

Diabetes Mellitus ◽

Lipid Metabolism ◽

Fasting Blood Glucose ◽

Akt Signaling ◽

Hepatic Glycogen ◽

High Dose ◽

Lipid Metabolism Disorder ◽

Metabolism Disorder ◽

Glucose And Lipid Metabolism ◽

Quercetin Treatment

Glucose and lipid metabolism disorder in diabetes mellitus often causes damage to multiple tissues and organs. Diabetes mellitus is beneficially affected by quercetin. However, its concrete mechanisms are yet to be fully elucidated. In our study, diabetes was induced in Sprague-Dawley rats by STZ injection. The rats were randomly divided into normal control, diabetic model, low-dose quercetin treatment, high-dose quercetin treatment, and pioglitazone treatment groups. Fasting blood glucose was collected to evaluate diabetes. Immunohistochemistry and fluorometric assay were performed to explore SIRT1. Akt levels were measured through immunoprecipitation and Western blot. After 12 weeks of quercetin treatment, the biochemical parameters of glucose and lipid metabolism improved to varying degrees. Hepatic histomorphological injury was alleviated, and hepatic glycogen content was increased. The expression and activity of hepatic SIRT1 were enhanced, and Akt was activated by phosphorylation and deacetylation. These results suggested that the beneficial effects of quercetin on glucose and lipid metabolism disorder are probably associated with the upregulated activity and protein level of SIRT1 and its influence on Akt signaling pathway. Hence, quercetin shows potential for the treatment of glucose and lipid metabolism disorder in diabetes mellitus.

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Angelica sinensis polysaccharide regulates glucose and lipid metabolism disorder in prediabetic and streptozotocin-induced diabetic mice through the elevation of glycogen levels and reduction of inflammatory factors

Food & Function ◽

10.1039/c4fo00859f ◽

2015 ◽

Vol 6 (3) ◽

pp. 902-909 ◽

Cited By ~ 33

Author(s):

Kaiping Wang ◽

Peng Cao ◽

Weizhi Shui ◽

Qiuxiang Yang ◽

Zhuohong Tang ◽

...

Keyword(s):

Lipid Metabolism ◽

Inflammatory Factors ◽

Angelica Sinensis ◽

Diabetic Mice ◽

Lipid Metabolism Disorder ◽

Metabolism Disorder ◽

Glucose And Lipid Metabolism

Hypoglycemic and hypolipidemic effects of ASP in prediabetic and T2DM mice.

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The role of miR-320 in glucose and lipid metabolism disorder-associated diseases

International Journal of Biological Sciences ◽

10.7150/ijbs.53419 ◽

2021 ◽

Vol 17 (2) ◽

pp. 402-416

Author(s):

Hengzhi Du ◽

Yanru Zhao ◽

Zhongwei Yin ◽

Dao Wen Wang ◽

Chen Chen

Keyword(s):

Lipid Metabolism ◽

Lipid Metabolism Disorder ◽

Associated Diseases ◽

Metabolism Disorder ◽

Glucose And Lipid Metabolism

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Renal Injury by SARS-CoV-2 Infection: A Systematic Review

Kidney Diseases ◽

10.1159/000512683 ◽

2020 ◽

pp. 1-11

Author(s):

Mo Wang ◽

Huaying Xiong ◽

Han Chen ◽

Qiu Li ◽

Xiong Zhong Ruan

Keyword(s):

Lipid Metabolism ◽

Immune Tolerance ◽

Renal Injury ◽

Thrombus Formation ◽

Renal Involvement ◽

Systemic Effects ◽

Lipid Metabolism Disorder ◽

Immune Clearance ◽

Metabolism Disorder ◽

Glucose And Lipid Metabolism

Background: SARS-CoV-2 infection can cause renal involvement, and severe renal dysfunction is more common among patients with chronic comorbid conditions, especially patients with chronic kidney disease. Angiotensin-converting enzyme 2 (ACE2) has been proven to be the major receptor of SARS-CoV-2 in kidneys, suggesting that ACE2-related changes may be involved in renal injury during the infection. In this review, we systematically reviewed the literature to summarize findings on the mechanism of renal injury caused by SARS-COV-2 infection, in order to provide a theoretical basis for renal protection therapy. Summary: For patients with SARS-CoV-2 infection, renal injury mainly manifests as increased serum creatinine, variable degrees of proteinuria and hematuria, and radiographic abnormalities of the kidneys. In this review, we summarize the pathogenesis of renal injury deriving from SARS-CoV-2 infection by focusing on its etiology, pathology, and clinical manifestations. The virus causes kidney injury by either direct infection or systemic effects, including host immune clearance and immune tolerance disorders, endothelium-mediated vasculitis, thrombus formation, glucose and lipid metabolism disorder, and hypoxia. Key Messages: Renal injury by SARS-CoV-2 is the result of multiple factors. Via highly expressed ACE2 in renal tissue, SARS-CoV-2 infection fundamentally initiates a mechanism of renal injury. Systemic effects such as host immune clearance and immune tolerance disorders, endothelial cell injury, thrombus formation, glucose and lipid metabolism disorder, and hypoxia aggravate this renal injury.

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Hepatic Lipid Metabolism Disorder and Atherosclerosis

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530322666211220110810 ◽

2021 ◽

Vol 22 ◽

Author(s):

Sen Zhang ◽

Fenfang Hong ◽

Chen Ma ◽

Shulong Yang

Keyword(s):

Lipid Metabolism ◽

Experimental Studies ◽

Cholesterol Transport ◽

Hepatic Lipid Metabolism ◽

Lipid Metabolism Disorder ◽

Hepatic Lipid ◽

Metabolism Disorder ◽

Lipid Metabolism Disorders ◽

And Oxidative Stress

: Lipid metabolism disorder plays a fundamental role in the pathogenesis of atherosclerosis. As the largest metabolic organ of the human body, liver has a key role in lipid metabolism by influencing fat production, fat decomposition, and the intake and secretion of serum lipoproteins. Numerous clinical and experimental studies have indicated that the dysfunction of hepatic lipid metabolism is closely tied to the onset of atherosclerosis. However, the identity and functional role of hepatic lipid metabolism responsible for these associations remain unknown. This review presented that cholesterol synthesis, cholesterol transport, and the metabolism of triglyceride, lipoproteins, and fatty acids are all associated with hepatic lipid metabolism and atherosclerosis. Moreover, we also discussed the roles of gut microbiota, inflammatory response, and oxidative stress in the pathological association between hepatic lipid metabolism and atherosclerosis. These significant evidences support strongly that hepatic lipid metabolism disorders may increase the risk of atherosclerosis.

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Effects of Insulin Pathway on Glucose and Lipid Metabolism Disorder in Different Pathological Types of Colorectal Adenomas

Exploratory Research and Hypothesis in Medicine ◽

10.14218/erhm.2021.00018 ◽

2021 ◽

Vol 000 (000) ◽

pp. 000-000

Author(s):

Junli Shi ◽

Sijin Li ◽

Yuewen Qi ◽

Ping Li ◽

Weiluo Sun ◽

...

Keyword(s):

Lipid Metabolism ◽

Colorectal Adenomas ◽

Lipid Metabolism Disorder ◽

Insulin Pathway ◽

Metabolism Disorder ◽

Glucose And Lipid Metabolism

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High-Fat Diets-Induced Metabolic Disorders to Study Molecular Mechanism of Hyperlipidemia in Rats

3BIO: Journal of Biological Science, Technology and Management ◽

10.5614/3bio.2021.3.2.5 ◽

2021 ◽

Vol 3 (2) ◽

pp. 38-50

Author(s):

Harfi Maulana ◽

Ahmad Ridwan

Keyword(s):

Insulin Resistance ◽

Lipid Metabolism ◽

Cholesterol Homeostasis ◽

High Fat ◽

Lipid Metabolism Disorder ◽

Signaling Mechanism ◽

Signaling Systems ◽

Metabolism Disorder ◽

Lipid Metabolism Disorders ◽

Primary Literature

Hyperlipidemia is a lipid metabolism disorder occurring due to consumption of a high-fat diet (HFD), which contributes to atherosclerosis and cardiovascular disease development. HFD causes metabolic problems in Rodentia animals like human metabolic abnormalities, making it a popular model for studying the signaling systems involved. Hyperlipidemia is a condition in which the body's cholesterol levels elevate. In recent years, several studies have investigated the relationship between HFD feeding and hyperlipidemia and signaling pathways involved in cholesterol homeostasis. However, this signaling mechanism in lipid metabolism has not been fully explained, so additional analysis is needed. The present study aimed to investigate the mechanism that occurs from hyperlipidemia due to HFD feeding. The method used is a literature review approach following the PRISMA scheme for selecting the primary literature, including identification, screening, eligibility test, and inclusion. Eleven articles included primary literature with credibility (H-index) of 20, 33, 71, 92, 93, 162, 180, 192, and 332 (six articles from Q1 journals and five from Q2 journals). Long-term administration of HFD directly affects lipid metabolism, including an increase in the concentration of total cholesterol, triglycerides, LDL, and a decrease in HDL concentration, followed by an increase in body weight. In addition, HFD also disrupts adipose tissue and insulin resistance. The conclusion of this study is that HFD can cause hyperlipidemia either directly or indirectly by inducing insulin resistance, which contributes to lipid metabolism disorders.

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