7. Following biochemistry within the cell
2021 ◽
pp. 93-110
Keyword(s):
This chapter presents key advances in the study of individual molecules within cells. When one applies this individualistic methodology to biochemicals, one enters the realms of single-molecule biophysics. There are of course formidable technical challenges associated with single-molecule studies, not least of which is the signal-to-noise problem. The chapter discusses patch clamping, nanoscopes, and optical tweezers. Patch clamping provided insights into the protein machinery that controls the flow of ions in and out of cells. The chapter also examines cytokinesis, cytoskeletons, and motor proteins, and the use of Green Fluorescent Protein (GFP).
2001 ◽
Vol 2
(6)
◽
pp. 392-396
◽
2019 ◽
Vol 20
(6)
◽
pp. 1410
◽
2001 ◽
Vol 105
(1)
◽
pp. 55-63
◽
2017 ◽
Vol 114
(42)
◽
pp. 11052-11056
◽
2004 ◽
2001 ◽
pp. 338-352
2003 ◽
Vol 47
(12)
◽
pp. 3682-3687
◽
2006 ◽
Vol 284-285
◽
pp. 395-400
◽