Single-Molecule Surface-Enhanced Resonance Raman Spectroscopy of the Enhanced Green Fluorescent Protein EGFP

2006 ◽  
pp. 297-312
Author(s):  
Satoshi Habuchi ◽  
Johan Hofkens
Keyword(s):  
Raman Spectroscopy ◽  
Green Fluorescent Protein ◽  
Single Molecule ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Surface Enhanced ◽  
Green Fluorescent ◽  
Molecule Surface

Single-Molecule Surface Enhanced Resonance Raman Spectroscopy of the Enhanced Green Fluorescent Protein

2003 ◽  
Vol 125 (28) ◽  
pp. 8446-8447 ◽  
Author(s):  
Satoshi Habuchi ◽  
Mircea Cotlet ◽  
Roel Gronheid ◽  
Gunter Dirix ◽  
Jan Michiels ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Green Fluorescent Protein ◽  
Single Molecule ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Surface Enhanced ◽  
Green Fluorescent ◽  
Molecule Surface

Surface-Enhanced Resonance Raman Spectroscopy Signals from Single Myoglobin Molecules

2002 ◽  
Vol 56 (12) ◽  
pp. 1531-1537 ◽  
Author(s):  
Anna Rita Bizzarri ◽  
Salvatore Cannistraro
Keyword(s):  
Raman Spectroscopy ◽  
Single Molecule ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Metallic Surface ◽  
Large Cross Section ◽  
Low Concentrations ◽  
Surface Enhanced ◽  
Silver Colloidal Nanoparticles ◽  
Short Times

The extremely large cross-section available from metallic surface enhancement has been exploited to investigate the Raman spectrum of heme myoglobin adsorbed on silver colloidal nanoparticles at very low concentrations. The study has been performed on particles both in solution and immobilized onto a polymer-coated glass surface. In both the cases, we have observed striking temporal fluctuations in the surface-enhanced resonance Raman spectroscopy (SERRS) spectra collected at short times. A statistical analysis of the temporal intensity fluctuations and of the associated correlations of the Raman signals has allowed us to verify that the single molecule limit is approached. The possible connections of these fluctuations with the entanglement of the biomolecule within the local minima of its rough energy landscape is discussed.

scholarly journals Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

2006 ◽  
Vol 14 (21) ◽  
pp. 9815 ◽  
Author(s):  
Alberto Diaspro ◽  
Silke Krol ◽  
Barbara Campanini ◽  
Fabio Cannone ◽  
Giuseppe Chirico
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gfp Fluorescence ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

Homogeneity, Transport, and Signal Properties of Single Ag Particles Studied by Single-Molecule Surface-Enhanced Resonance Raman Scattering

2001 ◽  
Vol 105 (15) ◽  
pp. 3673-3679 ◽  
Author(s):  
C. Eggeling ◽  
J. Schaffer ◽  
C. A. M. Seidel ◽  
J. Korte ◽  
G. Brehm ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Resonance Raman Scattering ◽  
Ag Particles ◽  
Surface Enhanced ◽  
Molecule Surface
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