Outdated Red Blood Cells from the Blood Bank, for Determination of Antistreptolysin O Titers

1961 ◽  
Vol 35 (3_ts) ◽  
pp. 279-283
Author(s):  
Y. E. Crawford ◽  
R.P. Skinner ◽  
W.J. Lind ◽  
A. E. Heimann ◽  
D.E. Hutchings ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Bank

Measurement of hemoglobin oxygen saturation in capillaries

1987 ◽  
Vol 252 (5) ◽  
pp. H1031-H1040 ◽  
Author(s):  
M. L. Ellsworth ◽  
R. N. Pittman ◽  
C. G. Ellis
Keyword(s):  
Light Intensity ◽  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Optical Density ◽  
Blood Cells ◽  
Striated Muscle ◽  
Cheek Pouch ◽  
Retractor Muscle ◽  
Hamster Cheek Pouch

We present a computer-aided videodensitometric method for the determination of oxygen saturation in red blood cells flowing through capillaries of the hamster cheek pouch retractor muscle. The optical density (OD) of red blood cells is determined at two wavelengths. At the first, 431 nm, there is a maximum difference between absorption by oxygen deoxyhemoglobin. At the second, 420 nm, absorption is equal for the two absorbing species (isosbestic wavelength). In capillaries of the retractor muscle a relationship between oxygen saturation (S) and the following OD ratio was obtained as S = -1.71 (OD431/OD420) + 2.20. The error (95% confidence interval) in oxygen saturation associated with a determination of the OD ratio is estimated to be +/- 4.8%. The computerization of the method employs a frame-by-frame analysis of the light intensity over a selected capillary segment. The light intensity waveform along the segment is digitized and the minimum (I) and maximum (I0) light intensities are used to compute an optical density (OD = log10 [I0/I]). These minimum and maximum intensities correspond to the presence and absence of a red blood cell, respectively. The method permits the off-line analysis of videotaped scenes and provides a means of assessing the extent of temporal and spatial heterogeneity of oxygen saturation in selected capillary networks. The method has been developed for use in capillaries in transilluminated striated muscle but should be generally applicable to the measurement of capillary oxygen saturation in other tissues.

Age-Dependent Acoustic and Microelastic Properties of Red Blood Cells Determined by Vector Contrast Acoustic Microscopy

2012 ◽  
Vol 18 (3) ◽  
pp. 436-444 ◽  
Author(s):  
Esam T. Ahmed Mohamed ◽  
Albert E. Kamanyi ◽  
Mieczysław Pluta ◽  
Wolfgang Grill
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Mass Density ◽  
Healthy Person ◽  
Ultrasonic Waves ◽  
Acoustic Microscopy ◽  
Cell Parameters ◽  
Different Ages ◽  
The Individual

AbstractVariations of the mechanical properties of red blood cells that occur during their life span have long been an intriguing task for investigations. The research presented is based on noninvasive monitoring of red blood cells of different ages performed by scanning acoustic microscopy with magnitude and phase contrast. The characteristic signature of fixed cells from groups of three different ages fractionated according to mass density is obtained from the acoustic microscope images, with the data represented in polar graphs. The analysis of these data enables the determination of averaged values for the velocities of ultrasound propagating in the cells from the different groups ranging from (1,681 ± 16) m s−1in the youngest to (1,986 ± 20) m s−1in the oldest group. The determined bulk modulus varies with age from (3.04 ± 0.05) GPa to (4.34 ± 0.08) GPa. An approach to determine for an age-mixed population of red blood cells, collected from a healthy person, the age of the individual cells and the age dependence of the cell parameters including density, velocity, and attenuation of longitudinal polarized ultrasonic waves traveling in the cells is demonstrated.

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