A RAPID SIMPLE IMPROVED METHOD FOR THE PREPARATION OF Tc99m RED BLOOD CELLS FOR THE DETERMINATION OF RED CELL VOLUME

1973 ◽  
Vol 118 (4) ◽  
pp. 861-864 ◽  
Author(s):  
WILLIAM C. ECKELMAN ◽  
RICHARD C. REBA ◽  
SOLOMON N. ALBERT
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Cells ◽  
Red Cell ◽  
Improved Method ◽  
Red Cell Volume

Splenic red cell sequestration and blood volume measurements in conscious pigs

1985 ◽  
Vol 248 (3) ◽  
pp. R293-R301 ◽  
Author(s):  
J. P. Hannon ◽  
C. A. Bossone ◽  
W. G. Rodkey
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Physical Restraint ◽  
Red Cell ◽  
Red Cell Volume ◽  
Epinephrine Injection ◽  
Volume Measurements

When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.

Splanchnic blood volume in traumatic shock

1961 ◽  
Vol 200 (3) ◽  
pp. 614-618 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Traumatic Shock ◽  
Red Cell ◽  
Differential Distribution ◽  
Red Cell Volume ◽  
Tourniquet Shock

The distribution of radioiodinated plasma and radioiron-labeled red blood cells between the liver, intestine and spleen were determined during the induction and development of tourniquet shock in mice. The data obtained indicate that plasma and red blood cells are distributed differentially throughout the splanchnic vasculature such that plasma volume of liver, intestine and spleen remain depressed for the entire shock interval, as does splenic red cell volume. After an early decline, the red cell volume of liver and intestine become elevated to a level above control. This differential distribution of plasma and red cells in liver and intestine is attributed to alterations in peripherovascular tone and suggests that a venous component becomes prominent late in shock and may act to pool blood out of active circulation.

scholarly journals Ionic and osmotic equilibria of human red blood cells treated with nystatin.

1979 ◽  
Vol 74 (2) ◽  
pp. 157-185 ◽  
Author(s):  
J C Freedman ◽  
J F Hoffman
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Cells ◽  
Osmotic Coefficients ◽  
Virial Equation ◽  
Red Cell ◽  
Extracellular Solution ◽  
Red Cell Volume ◽  
Human Red Blood Cells ◽  
Cell Volumes

Human red blood cells have been incubated in the presence of nystatin, which allows Na and K, as well as Cl and pH to equilibrate rapidly when cell volume is set with external impermeant sucrose. The intracellular mean ionic activity coefficients, relative to values in the extracellular solution, for KCl and NaCl are 1.01 +/- 0.02 and 0.99 +/- 0.02 (SD, n = 10), respectively, and are independent of external pH, pH o, and of [sucrose]o. With nystatin the dependence of red cell volume on [sucrose]o deviates from ideal osmotic behavior by as much as a factor of three. A virial equation for the osmotic coefficient, phi, of human hemoglobin, Hb, accounts for the cell volumes, and is the same as that which describes Adair's measurements of phi Hb for Hb isolated from sheep and ox bloods. In the presence of nystatin the slope of the acid-base titration curve of the cells is independent of cell volume, implying that the charge on impermeant cellular solutes is independent of Hb concentration at constant pH. By modifying the Jacobs-stewart equations (1947. J. Cell. Comp. Physiol. 30: 79--103) with the osmotic coefficients of Hb and of salts, a nonideal thermodynamic model has been devised which predicts equilibrium Donnan ratios and red cell volume from the composition of the extracellular solution and from certain parameters of the cells. In addition to accounting for the dependence of cell volume on osmotic pressure, the model also describes accurately the dependence of Donnan ratios and cell volumes on pHo either in the presence or absence of nystatin.

Changes in Blood, Plasma and Red Cell Volume in the Male Rat, as a Function of Age

1957 ◽  
Vol 190 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Joseph F. Garcia
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Cells ◽  
The Body ◽  
High Rate ◽  
Male Rats ◽  
Male Rat ◽  
Red Cell ◽  
Red Cell Volume

Using the Fe59-labeled cell dilution technique, the blood, plasma and red cell volume and total circulating hemoglobin have been determined on 259 male rats varying in age from 1 to 340 days. An anemic period exists in the male rat which is maximal between 15 and 20 days of age. This anemia is observed in terms of a decrease in the volume of red blood cells per gram of rat as well as in hematocrit and hemoglobin concentration values. However, throughout this anemic period there is a continual increase in total red cell volume and, in fact, the daily gain of red blood cells per gram of rat is greater during this period than at any time thereafter. In spite of the very high rate of erythropoiesis occurring at this time the growth of the body mass as a whole exceeds it and so for a time anemia results.

Colloid osmotic pressure changes of dog's blood exposed to different mixtures of CO2 and air

1978 ◽  
Vol 44 (2) ◽  
pp. 254-257 ◽  
Author(s):  
Y. Kakiuchi ◽  
A. B. DuBois ◽  
D. Gorenberg
Keyword(s):  
Red Blood Cells ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Blood Cells ◽  
Freezing Point ◽  
Colloid Osmotic Pressure ◽  
Red Cell ◽  
Freezing Point Depression ◽  
Pressure Changes ◽  
Different Levels

Hansen's membrane manometer method for measuring plasma colloid osmotic pressure was used to obtain the osmolality changes of dogs breathing different levels of CO2. Osmotic pressure was converted to osmolality by calibration of the manometer with saline and plasma, using freezing point depression osmometry. The addition of 10 vol% of CO2 to tonometered blood caused about a 2.0 mosmol/kg H2O increase of osmolality, or 1.2% increase of red blood cell volume. The swelling of the red blood cells was probably due to osmosis caused by Cl- exchanged for the HCO3- which was produced rapidly by carbonic anhydrase present in the red blood cells. The change in colloid osmotic pressure accompanying a change in co2 tension was measured on blood obtained from dogs breathing different CO2 mixtures. It was approximately 0.14 mosmol/kg H2O per Torr Pco2. The corresponding change in red cell volume could not be calculated from this because water can exchange between the plasma and tissues.

Determination of Red-Cell Volume with a Nonradioactive Marker (Sodium Fluorescein) in Humans Using Flow Cytometry

1997 ◽  
Vol 24 (3) ◽  
pp. 132-137
Author(s):  
M. Haller ◽  
H. Dressel ◽  
M. Thiel ◽  
V. Orth ◽  
H. Brechtelsbauer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Volume ◽  
Sodium Fluorescein ◽  
Red Cell ◽  
Red Cell Volume

Quantitative measurement of erythropoiesis in the dog

1960 ◽  
Vol 198 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S. M. Weissman ◽  
T. A. Waldmann ◽  
N. I. Berlin
Keyword(s):  
Life Span ◽  
Cell Survival ◽  
Cell Volume ◽  
Quantitative Measurement ◽  
Red Cells ◽  
Red Cell ◽  
Red Cell Volume ◽  
Cell Life ◽  
Red Cell Survival

The quantitative measurement of erythropoiesis requires the simultaneous determination of total red cell volume, rate of production of red cells and the red cell life span. The total red cell volume was measured with autologous Cr51-labeled red cells, the rate of production of red cells from the rate of disappearance of radioiron from the plasma and uptake by red cells, the red cell life span with C14-labeled glycine and the apparent red cell survival T1/2 with Cr51. The average total red cell volume of the dogs studied was 38.6 cc/kg; the plasma radioiron T1/2 was 66 minutes; the red cell radio-iron uptake was 80%; the serum iron was 102 µg/100 cc, and the plasma volume calculated from the peripheral hematocrit and total red cell volume was 46 cc/kg, and from the extrapolation to t0 of the radioiron disappearance was 48 cc/kg. From these figures the plasma iron turnover was calculated to be 0.63 mg/kg/day and the red cell iron renewal rate 1.26%/day. The average red cell life span was 108 days; the average apparent T1/2 of Cr51 red cell survival was 24.3 days; the average elution rate of Cr51 was 1.77%/day.

Experiences with the Radiochromium method for Determination of Red Cell Volume

1962 ◽  
Vol 14 (4) ◽  
pp. 355-367 ◽  
Author(s):  
R. Wennesland ◽  
E. Brown ◽  
J. Hopper ◽  
K. G. Scott ◽  
J. L. Hodges ◽  
...  
Keyword(s):  
Cell Volume ◽  
Red Cell ◽  
Red Cell Volume

Effect of polycythemia on vascular volume in the newborn dog

1984 ◽  
Vol 246 (6) ◽  
pp. H830-H837
Author(s):  
M. H. Leblanc ◽  
K. Pate
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Evans Blue ◽  
Whole Blood ◽  
Blood Cells ◽  
Exchange Transfusion ◽  
Red Cell ◽  
Vascular Volume

The effect of polycythemia [hematocrit (Hct) 64-80] on blood volume (BV) was studied in 27 unanesthetized, splenectomized newborn dogs (age 6-14 days, postsplenectomy 5-13 days). Normovolemic polycythemia (N) was induced in nine pups by exchange transfusion with 75 ml/kg of adult, packed (to Hct 95) red blood cells (RBC). Hypervolemic polycythemia (H) was induced in 11 pups by transfusion of RBC (50 ml/kg). Seven pups received exchange transfusion with 75 ml/kg of whole blood and served as controls (C). Red cell volume (RCV, 51CrRBC) and plasma volume (PV, 125I-fibrinogen and Evans blue) were measured prior to and at 1, 2, and 4 h after transfusion, before the pups received fluid orally. The pups were fed 8 ml X kg-1 X h-1 after 4 h, and measurements were repeated at 8 and 24 h. BV fell in C prior to 4 h by 10 +/- 4% (SD) (P less than 0.01) and then rose to initial levels. BV rose in the N pups by 17 +/- 9 (P less than 0.01), 14 +/- 5 (P less than 0.01), 9 +/- 10 (P less than 0.1), 17 +/- 9 (P less than 0.01), and 31 +/- 17% (P less than 0.01) at 1, 2, 4, 8, and 24 h post transfusion. BV rose in the H pups by 41 +/- 8, 35 +/- 10, 23 +/- 11, 27 +/- 6, and 43 +/- 9% (all P less than 0.01). Thus newborn dogs with induced N or H equilibrate rapidly to a BV significantly higher than C levels.(ABSTRACT TRUNCATED AT 250 WORDS)

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