A novel dextrin produced by the enzymatic reaction of 6-α-glucosyltransferase Ⅱ: Practical advantages of the novel dextrin as a food modifier

Author(s):  
Rika Sumida ◽  
Seiichiro Kishishita ◽  
Akiko Yasuda ◽  
Manabu Miyata ◽  
Akiko Mizote ◽  
...  

ABSTRACT High-molecular-weight dextrin (WS-1000) was produced from waxy corn starch and enzymatically modified to link glucose by α-1,6 glycosidic bond at the terminal point of the glucose chain, forming MWS-1000. In this study, the physical properties of MWS-1000 were characterized, and the advantages of its use as a food modifier were described. From rheological and calorimetric studies, it was found that MWS-1000 does not undergo retrogradation, but it does not prevent the retrogradation of WS-1000, suggesting that they have no intermolecular interaction in solution. Investigation of the effect of MWS-1000 on the viscoelasticity of gelatinized wheat starch showed that in the linear viscoelastic region, storage modulus decreased and tan δ increased with increase in replaced MWS-1000 content. In addition, it was confirmed that gelatinized starch containing MWS-1000 showed viscoelastic behavior similar to that of commercially available custard cream.

Author(s):  
Akiko Yasuda ◽  
Manabu Miyata ◽  
Osamu Sano ◽  
Tatsufumi Sogo ◽  
Seiichiro Kishishita ◽  
...  

Abstract We prepared a high-molecular-weight modified dextrin (MWS-1000) from a partial hydrolysate of waxy corn starch with a weight average molecular weight of 1 × 106 (WS-1000) using Paenibacillus alginolyticus PP710 α-glucosyltransferase. The gel permeation chromatography showed that the weight average molecular weight of MWS-1000 was almost the same as that of WS-1000. The side chain length of WS-1000 and MWS-1000 after isomaltodextranase digestion were also shown to be similar to each other by high performance anion exchange chromatography with pulsed amperometric detection. Since MWS-1000 confirmed the presence of α-1,6 bonds by enzyme digestibility, methylation, and 1H-NMR analyses, it was presumed that the structure of MWS-1000 was based on the introduction of α-1,6 glucosyl residues at the non-reducing ends of the partial hydrolysate of waxy corn starch. Furthermore, the MWS-1000 solution was not retrograded even during refrigerated storage or after repeated freeze-thaw cycles.


2013 ◽  
Vol 61 (2) ◽  
pp. 379-386 ◽  
Author(s):  
Hanyu Yangcheng ◽  
Hongxin Jiang ◽  
Michael Blanco ◽  
Jay-lin Jane

2019 ◽  
Vol 51 ◽  
pp. 350-358 ◽  
Author(s):  
Qing-Yu Yang ◽  
Xuan-Xuan Lu ◽  
Yong-Zhi Chen ◽  
Zhi-Gang Luo ◽  
Zhi-Gang Xiao

2017 ◽  
Vol 70 ◽  
pp. 201-210 ◽  
Author(s):  
Marzena Włodarczyk-Stasiak ◽  
Artur Mazurek ◽  
Jerzy Jamroz ◽  
Mieczysław Hajnos ◽  
Zofia Sokołowska

2000 ◽  
Vol 48 (10) ◽  
pp. 4469-4476 ◽  
Author(s):  
Kenneth S. Lewen ◽  
Iris H. McCormick ◽  
Paul Molitor ◽  
Shelly J. Schmidt ◽  
Thomas M. Eads

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