scholarly journals EVALUATION OF THE RELEASE RATE OF A CHEMOTHERAPEUTIC AGENT INCORPORATED AS A COATING ON A BIODEGRADABLE URETERAL STENT. IN VITRO STUDY

2021 ◽  
Vol 108 (Supplement_3) ◽  
Author(s):  
M Soto Gallardo ◽  
J E de la Cruz Conty ◽  
S D Aznar Cervantes ◽  
J L Cenis Anadón ◽  
F M Sánchez Margallo ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Release Rate ◽  
In Vitro Study ◽  
Dip Coating ◽  
Polymeric Matrix ◽  
Ureteral Stent ◽  
Artificial Urine ◽  
Dip Coating Technique ◽  
Mean Concentration

Abstract INTRODUCTION To assess the release rate of Mitomycin C (MMC) after its adherence to a biodegradable ureteral stent (BraidStent™-MMC) by dip coating in order to evaluate its potential application as an adjuvant treatment for upper tract urothelial carcinoma. MATERIAL AND METHODS The dip coating technique is applied to a total of 10 fragments of the BraidStent™ catheter which has a polymeric matrix as a coating. Each 3 cm fragment is immersed 10 times in pure MMC crystallising in methanol and finally obtaining the formation of microlayers on its surface. After drying, each fraction of the stent is immersed in 5 ml of artificial urine to study its interaction with this medium. The samples remain in an orbital shaker at 36ºC and the medium is exchanged under sterile conditions after 12, 24, 48, 48, 72, 96 and 120h. At each replacement, the remaining urine is analysed by HPLC-DAD to quantify the presence of the cytotoxic agent.    RESULTS During the first 12h, MMC is completely released reaching a mean concentration of 52.22 mg/L. Comparing this result with those previously obtained with the first BraidStent ™ -MMC formulation (10.82mg/L), it is determined that this technique allows to increase the release rate up to 5 times. CONCLUSIONS The release rate of Mitomycin C from a biodegradable ureteral stent is increased by integrating a polymeric matrix with microlayers in its coating. Further trials are needed to achieve the therapeutic dose for clinical application in oncology patients.

scholarly journals The effect of platelet-rich fibrin on normal dermal fibroblast proliferation after mitomycin-c treatment: An in vitro study

2021 ◽  
Vol 62 ◽  
pp. 473-476
Author(s):  
Ishandono Dachlan ◽  
Hendy Satrya Kurniawan ◽  
Aditya Wicaksana ◽  
Aditya Rifqi Fauzi ◽  
Firdian Makrufardi ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Dermal Fibroblast ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibroblast Proliferation ◽  
Platelet Rich Fibrin

Anti-Reflux Ureteral Stent with Polymeric Flap Valve Using Three-Dimensional Printing: An In Vitro Study

2015 ◽  
Vol 29 (8) ◽  
pp. 933-938 ◽  
Author(s):  
Chang-Ju Park ◽  
Hyeon-Woo Kim ◽  
Sangdo Jeong ◽  
Seungwan Seo ◽  
Yangkyu Park ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ureteral Stent ◽  
Three Dimensional Printing ◽  
Dimensional Printing ◽  
Flap Valve

Effect of Mitomycin C on Keloid Fibroblasts: An In Vitro Study

2003 ◽  
Vol 50 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Richard Simman ◽  
Hashim Alani ◽  
Frances Williams
Keyword(s):  
Mitomycin C ◽  
In Vitro Study ◽  
Vitro Study ◽  
Keloid Fibroblasts

scholarly journals Development and Characterization of Bioactive Glass Containing Composite Coatings with Ion Releasing Function for Antibiotic-Free Antibacterial Surgical Sutures

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 423 ◽  
Author(s):  
Francesca Ciraldo ◽  
Kristin Schnepf ◽  
Wolfgang Goldmann ◽  
Aldo Boccaccini
Keyword(s):  
Bioactive Glass ◽  
Composite Coatings ◽  
Antibacterial Properties ◽  
Dip Coating ◽  
Carbonate Apatite ◽  
Polymeric Layer ◽  
Surgical Sutures ◽  
Dip Coating Technique ◽  
Doped Glass

Resorbable (Vicryl® Plus) sutures were coated with zinc-doped glass (Zn-BG) and silver-doped ordered mesoporous bioactive glass (Ag-MBG) particles by a dip coating technique. A multilayer approach was used to achieve robust coatings. The first coating was a polymeric layer (e.g., PCL or chitosan) and the second one was a composite made of BG particles in a polymer matrix. The coatings were characterized in terms of morphology by scanning electron microscopy (SEM), in vitro bioactivity, and antibacterial properties. Chitosan/Ag-MBG coatings showed the ability to form hydroxyl-carbonate-apatite on their surfaces after immersion in SBF. An antibacterial effect against Gram (+) and Gram (-) bacteria was confirmed, highlighting the potential application of the coated sutures for antibiotic-free approaches.

scholarly journals Preparation and Characterization of MUC-30-Loaded Polymeric Micelles against MCF-7 Cell Lines Using Molecular Docking Methods and In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Norased Nasongkla ◽  
Patoomratana Tuchinda ◽  
Bamroong Munyoo ◽  
Komgrit Eawsakul
Keyword(s):  
Molecular Docking ◽  
Cytotoxic Activity ◽  
Release Rate ◽  
Water Solubility ◽  
Polymeric Micelles ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
Breast Cancer Chemotherapy ◽  
Mcf 7

MUC-30 is a hydrophobic compound which is active against the MCF-7 cancer cell line. In this study, MUC-30 was loaded in polymeric micelles to improve the water solubility and release rate. For prolonged MUC-30 release, MUC-30 was encapsulated in polymeric micelles using PEG-b-PLA and PEG-b-PCL as materials. Micelles prepared with 1 : 9 w per w ratios by film hydration achieved the highest entrapment efficiency (EE%). The EE% of MUC-30-loaded PEG-b-PCL micelles was approximately 30% greater than that of PEG-b-PLA micelles, due to the different H-bond formations between MUC-30 and the polymer membrane (PCL, 4; PLA, 3). The cytotoxic activity of MUC-30 against EGFR theoretically presented 399.31 nM (IC50 = 282.26 ng/mL) by molecular docking. In vitro cytotoxic activity of MUC-30 was confirmed by MTT assay. MUC-30 (IC50 = 11 ± 0.39 ng/mL) showed three-fold higher activity over MUC-30-loaded PEG-b-PLA micelles (IC50 = 37 ± 1.18 ng/mL) and two-fold higher over PEG-b-PCL micelles (IC50 = 75 ± 3.97 ng/mL). This was due to the higher release rate of MUC-30 from PEG-b-PLA micelles compared to PEG-b-PCL micelles. Therefore, MUC-30-loaded PEG-b-PLA micelles could be a promising candidate for breast cancer chemotherapy.

scholarly journals In Vitro Study of Combined Application of Bevacizumab and 5-Fluorouracil or Bevacizumab and Mitomycin C to Inhibit Scar Formation in Glaucoma Filtration Surgery

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Yuanyuan Zhang ◽  
Shaopin Zhu ◽  
Xun Xu ◽  
Lei Zuo
Keyword(s):  
Mitomycin C ◽  
In Vitro Study ◽  
Drug Combinations ◽  
Vitro Study ◽  
Type I ◽  
Subconjunctival Injection ◽  
Filtration Surgery ◽  
Glaucoma Filtration Surgery ◽  
Combined Application

This is an in vitro study conducted to observe the safety and antiscarring effects of combined application of bevacizumab (BVZ) and 5-fluorouracil (5-Fu) or BVZ and mitomycin C (MMC) during glaucoma filtration surgery (GFS). The cytotoxicity of drug combinations in human Tenon’s fibroblasts (HTFs) and human umbilical vein endothelial cells (HUVECs) was evaluated. Their effects on the levels of vascular endothelial growth factor (VEGF) in HUVECs, cell proliferation and migration in HTFs, and the expression of collagen type I alpha 1 (Col1A1) gene in HTFs were evaluated. In addition, the effects of combined drugs on VEGF(R) mRNA in HTFs were detected to explore the possible underlying drug mechanisms. The results showed that BVZ combined with 5-Fu demonstrated more significant antiscarring effects than BVZ or 5-Fu alone. However, the inhibitory effects of BVZ combined with MMC were similar to those of MMC alone. The cytotoxicity of the drug combinations was significantly greater than that of single drug, suggesting that combined application of BVZ and antimetabolites after GFS was safer when applied at different sites (such as subconjunctival injection at bilateral sides of the filtering bleb) or at varied time points.

scholarly journals HEPARIN AS AN ANTIBACTERIAL COATING ON BIODEGRADABLE URETERAL STENTS: EXPERIMENTAL STUDY OF BRAIDSTENT®-H

2021 ◽  
Vol 108 (Supplement_3) ◽  
Author(s):  
J E de la Cruz Conty ◽  
A Budía Alba ◽  
J L Sanz Migueláñez ◽  
J A Galán Llopis ◽  
T Fernández Aparicio ◽  
...  
Keyword(s):  
Dip Coating ◽  
Ureteral Stents ◽  
Biodegradable Stent ◽  
Antibacterial Coating ◽  
Heparin Coating ◽  
Double J Stent ◽  
Effective Inhibition ◽  
Artificial Urine

Abstract INTRODUCTION The aim of this study is to assess the effectiveness of heparin to inhibit the development of early bacteriuria as a coating for biodegradable ureteral stents. MATERIAL AND METHODS The BraidStent®-H biodegradable stent, whose heparin coating is incorporated by dip coating, was chosen for this study. Twenty-four swine were randomly divided into two groups: 12 animals underwent unilateral placement of the BraidStent®-H and 12 were fitted with a standard double-j stent (DJS). Bacteriuria is comparatively analyzed over time by consecutive urine sampling at 0, 1, 3, 6, 12, 24 and 48 hours. In addition, the concentration of heparin released in vitro in artificial urine at 0, 3, 6, 12, 24, 48, 72, 92 and 120 hours is determined via ELISA. RESULTS BraidStent®-H generates a significantly lower bacteriuria rate than a DJS at 6 and 12 hours. Heparin coating shows a significant delaying effect on the onset of bacteriuria, reaching 100% of the animals at 48 hours, compared to the DJS, which takes place at 6 hours. ELISA results reveal the presence of heparin in urine for a total of 72 hours. The coating does not affect the degradation of the device, which is completed at 6 weeks. CONCLUSIONS Heparin evidences an effective inhibition of early bacteriuria, showing its potential as an antibacterial coating for biodegradable ureteral stents. Future studies should focus on the development of long-term heparin coatings for biodegradable materials.

Sign in / Sign up

Export Citation Format

Share Document