Immunohistochemically demonstrated glucose-6-phosphate dehydrogenase, γ-glutamyl transpeptidase, ornithine decarboxylase and glutathione S-transferase enzymes: absence of direct correlation with cell proliferation in rat liver putative preneoplastic lesions

1986 ◽  
Vol 7 (9) ◽  
pp. 1419-1424 ◽  
Author(s):  
Malcolm A. Moore ◽  
Toshikazu Nakamura ◽  
Nobuyuki Ito
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Rat Liver ◽  
Glutathione S Transferase ◽  
Preneoplastic Lesions ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Glutamyl Transpeptidase

Lack of potential of low dose N-nitrosodimethylamine to induce preneoplastic lesions, glutathione S-transferase placental form-positive foci, in rat liver

2005 ◽  
Vol 222 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Shoji Fukushima ◽  
Hideki Wanibuchi ◽  
Keiichirou Morimura ◽  
Dai Nakae ◽  
Hiroyuki Tsuda ◽  
...  
Keyword(s):  
Rat Liver ◽  
Low Dose ◽  
Glutathione S Transferase ◽  
Preneoplastic Lesions

Enhancing effects of mustard oil on preneoplastic hepatic foci development in Wistar rats

2003 ◽  
Vol 22 (2) ◽  
pp. 51-55 ◽  
Author(s):  
Yogeshwer Shukla ◽  
Annu Arora
Keyword(s):  
Wistar Rats ◽  
Dietary Habits ◽  
Liver Weight ◽  
Mustard Oil ◽  
Contributory Factor ◽  
Oil Consumption ◽  
Glutathione S Transferase ◽  
Preneoplastic Lesions ◽  
Altered Hepatic Foci ◽  
Glutamyl Transpeptidase

Dietary habits are known to be the major contributory factor in the development of cancer. Mustard oil, which is extensively used in India and elsewhere as a frying and cooking medium, is reported to induce an inflammatory response. The development of altered hepatic foci is an early carcinogenic change in rat liver in diethylnitrosamine (DEN)-induced hepatocarcinogenesis. In the present study, the development of preneoplastic lesions was observed following administration of mustard oil (0.5 mL/day for 8 weeks) in DEN-initiated and partially hepatomized Wistar rats. A significant decrease in the relative and absolute liver weight of mustard oil-exposed rats was recorded. The results revealed a significant increase in the number and area of placental glutathione S-transferase (GST-P) and g-glutamyl transpeptidase (GGT)-positive foci in mustard oil-administered animals. The GST-P and GGT-positive foci were more prominent in the animals given boiled (up to 3008C for 3 hours) mustard oil in comparison to the animals given fresh mustard oil. These results indicate the possible tumourigenic risk associated with mustard oil consumption.

Genotoxicity and Cell Proliferative Activity of 3-Chloro-4-(Dichloromethyl)-5-Hydroxy-2(5H)-Furanone [MX] in Rat Glandular Stomach

1992 ◽  
Vol 25 (11) ◽  
pp. 341-345 ◽  
Author(s):  
C. Furihata ◽  
M. Yamashita ◽  
N. Kinae ◽  
T. Matsushima
Keyword(s):  
Cell Proliferation ◽  
Body Weight ◽  
Dna Synthesis ◽  
Ornithine Decarboxylase ◽  
Tap Water ◽  
Fold Increase ◽  
Single Strand ◽  
Glandular Stomach ◽  
Decarboxylase Activity ◽  
Pyloric Mucosa

MX is a strong direct acting mutagen on Salmonella typhimurium TA100 and is present in chlorinated tap water which contains organic compounds. MX was administered orally to 7-week-old male F344 rats, and its geno-toxicity in the pyloric mucosa of stomach was examined by analysis of DNA single strand scissions by the alkaline elution method. The effect of MX on cell proliferation was examined by assays of the inductions of replicative DNA synthesis and ornithine decarboxylase. MX at closes of 20-48 mg/kg body weight induced DNA single strand scissions dose-dependently (p<0.02) in the pyloric mucosa of the stomach 2 h after its administration. Moreover at doses of 10-60 mg/kg body weight, it induced up to 21-fold increase in replicative DNA synthesis (p<0.01) 16 h after its administration. At doses of 10-60 mg/kg body weight, it induced up to 100-fold increase in ornithine decarboxylase activity with a maximum 16 h after its administration. These results suggest that MX is genotoxic and induces cell proliferation in the glandular stomach of rats.

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