An Automated Device for In Situ Pre-Column Derivatization and Injection of Amino Acids for HPLC Analysis

1983 ◽  
Vol 21 (11) ◽  
pp. 503-507 ◽  
Author(s):  
J. C. Hodgin ◽  
P. Y. Howard ◽  
D. M. Ball ◽  
C. Cloete ◽  
L. De Jaeger
Keyword(s):  
Amino Acids ◽  
Hplc Analysis

HPLC Analysis of Racemic Amides, Ureas, and Hydantoins Derived from Amino Acids on Silica-Based Chiral Columns

1991 ◽  
Vol 29 (10) ◽  
pp. 419-422 ◽  
Author(s):  
E. Aziz ◽  
J. S. Davies ◽  
G. Llewellyn ◽  
S. Szajda ◽  
R. S. Ward ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hplc Analysis

scholarly journals Determination of amino acids in human biological fluids by high-performance liquid chromatography: critical review

Amino Acids ◽  
2021 ◽  
Author(s):  
Grażyna Gałęzowska ◽  
Joanna Ratajczyk ◽  
Lidia Wolska
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Analytical Techniques ◽  
Epidemiological Studies ◽  
Preparation Conditions

AbstractThe quantitation and qualification of amino acids are most commonly used in clinical and epidemiological studies, and provide an excellent way of monitoring compounds in human fluids which have not been monitored previously, to prevent some diseases. Because of this, it is not surprising that scientific interest in evaluating these compounds has resurfaced in recent years and has precipitated the development of a multitude of new analytical techniques. This review considers recent developments in HPLC analytics on the basis of publications from the last few years. It helps to update and systematize knowledge in this area. Particular attention is paid to the progress of analytical methods, pointing out the advantages and drawbacks of the various techniques used for the preparation, separation and determination of amino acids. Depending on the type of sample, the preparation conditions for HPLC analysis change. For this reason, the review has focused on three types of samples, namely urine, blood and cerebrospinal fluid. Despite time-consuming sample preparation before HPLC analysis, an additional derivatization technique should be used, depending on the detection technique used. There are proposals for columns that are specially modified for amino acid separation without derivatization, but the limit of detection of the substance is less beneficial. In view of the fact that amino acid analyses have been performed for years and new solutions may generate increased costs, it may turn out that older proposals are much more advantageous.

A facile one pot route for the synthesis of imide tethered peptidomimetics

2016 ◽  
Vol 14 (2) ◽  
pp. 556-563 ◽  
Author(s):  
Veladi Panduranga ◽  
Girish Prabhu ◽  
Roopesh Kumar ◽  
Basavaprabhu Basavaprabhu ◽  
Vommina V. Sureshbabu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Efficient Method ◽  
One Pot ◽  
Protected Amino Acid

A simple and efficient method for the synthesis of N,N’-orthogonally protected imide tethered peptidomimetics is presented. The imide peptidomimetics were synthesized by coupling the in situ generated selenocarboxylate of Nα-protected amino acids with Nα-protected amino acid azides in good yields.

In situ studies on alginic acid synthesis and other aspects of the metabolism of Laminaria digitata

1972 ◽  
Vol 50 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Johan A. Hellebust ◽  
Arne Haug
Keyword(s):  
Amino Acids ◽  
Time Course ◽  
Alginic Acid ◽  
Acid Synthesis ◽  
Short Term ◽  
Fresh Weight Basis ◽  
Mannuronic Acid ◽  
Guluronic Acid ◽  
Rate Of Photosynthesis

Amino acids, particularly alanine and aspartate, become more strongly labeled than mannitol in short-term 14C-photoassimilation experiments. The amino acids are the most likely sources of carbon for alginic acid synthesis and respiration in the dark, in contrast to mannitol, which appears to be relatively unavailable. Temperature is very important in determining the rate of loss of recent photoassimilate in L. digitata. The rate of photosynthesis, on a fresh weight basis, is much higher for blades than for stipes.The time course for incorporation of photoassimilated carbon into alginate differs for the stipe and blade both in light and dark periods. Very little 14C enters alginate in blades in the dark, while alginate in stipes acquires considerable amounts of activity during dark periods. Alginate in both blade and stipe acquires 14C predominantly in mannuronic acid residues of their alginate during short-term photoassimilation periods, while guluronic acid residues become relatively more rapidly labeled during dark periods.

scholarly journals Innentitelbild: In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with FluorescentD-Amino Acids (Angew. Chem. 50/2012)

2012 ◽  
Vol 124 (50) ◽  
pp. 12546-12546
Author(s):  
Erkin Kuru ◽  
H. Velocity Hughes ◽  
Pamela J. Brown ◽  
Edward Hall ◽  
Srinivas Tekkam ◽  
...  
Keyword(s):  
Amino Acids ◽  
Live Bacteria

The entry of acidic amino acids into brain and CSF during development, using in situ perfusion in the rat

1995 ◽  
Vol 90 (1-2) ◽  
pp. 151-158 ◽  
Author(s):  
Hameed Al-Sarraf ◽  
Jane E. Preston ◽  
Malcolm B. Segal
Keyword(s):  
Amino Acids ◽  
In Situ Perfusion ◽  
Acidic Amino Acids

Phytates reduce uptake of leucine and glutamate but not lysine and glucose from the intestinal lumen of chickens: Short communication

2008 ◽  
Vol 56 (4) ◽  
pp. 511-514 ◽  
Author(s):  
Edward Onyango ◽  
Elikplimi Asem ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Short Communication ◽  
Intestinal Lumen ◽  
Depressive Effect ◽  
Leucine Uptake ◽  
Small Intestinal

An investigation into the influence of phytates on the in situ absorption of amino acids (lysine, glutamate and leucine) and glucose from the intestinal lumen of 3-week-old chickens was carried out. Birds were anaesthetised and the intestines exteriorised. Uptake of 5 mM of each nutrient over a 4-min period was measured in the presence of four phytate concentrations (0, 50, 250 and 500 mM). Five birds were used for each nutrient at each concentration of phytate tested. Leucine uptake decreased linearly (P < 0.001) and that of glutamate showed a tendency to decrease (P = 0.055) as the phytate concentration increased. Absorption of lysine and glucose were unaffected by the presence of phytate. In conclusion, phytate in the small intestinal lumen exerted a depressive effect on the absorption of specific free amino acids from the lumen. Its depressive effect was greatest for leucine followed by glutamate, and phytate had little effect on the absorption of lysine.

scholarly journals Genomics, Exometabolomics, and Metabolic Probing Reveal Conserved Proteolytic Metabolism of Thermoflexus hugenholtzii and Three Candidate Species From China and Japan

2021 ◽  
Vol 12 ◽  
Author(s):  
Scott C. Thomas ◽  
Devon Payne ◽  
Kevin O. Tamadonfar ◽  
Cale O. Seymour ◽  
Jian-Yu Jiao ◽  
...  
Keyword(s):  
United States ◽  
Amino Acids ◽  
Draft Genome ◽  
The United States ◽  
Pentose Phosphate ◽  
High Abundance ◽  
Comparative Genomic ◽  
Labeled Compounds ◽  
Geothermal Systems

Thermoflexus hugenholtzii JAD2T, the only cultured representative of the Chloroflexota order Thermoflexales, is abundant in Great Boiling Spring (GBS), NV, United States, and close relatives inhabit geothermal systems globally. However, no defined medium exists for T. hugenholtzii JAD2T and no single carbon source is known to support its growth, leaving key knowledge gaps in its metabolism and nutritional needs. Here, we report comparative genomic analysis of the draft genome of T. hugenholtzii JAD2T and eight closely related metagenome-assembled genomes (MAGs) from geothermal sites in China, Japan, and the United States, representing “Candidatus Thermoflexus japonica,” “Candidatus Thermoflexus tengchongensis,” and “Candidatus Thermoflexus sinensis.” Genomics was integrated with targeted exometabolomics and 13C metabolic probing of T. hugenholtzii. The Thermoflexus genomes each code for complete central carbon metabolic pathways and an unusually high abundance and diversity of peptidases, particularly Metallo- and Serine peptidase families, along with ABC transporters for peptides and some amino acids. The T. hugenholtzii JAD2T exometabolome provided evidence of extracellular proteolytic activity based on the accumulation of free amino acids. However, several neutral and polar amino acids appear not to be utilized, based on their accumulation in the medium and the lack of annotated transporters. Adenine and adenosine were scavenged, and thymine and nicotinic acid were released, suggesting interdependency with other organisms in situ. Metabolic probing of T. hugenholtzii JAD2T using 13C-labeled compounds provided evidence of oxidation of glucose, pyruvate, cysteine, and citrate, and functioning glycolytic, tricarboxylic acid (TCA), and oxidative pentose-phosphate pathways (PPPs). However, differential use of position-specific 13C-labeled compounds showed that glycolysis and the TCA cycle were uncoupled. Thus, despite the high abundance of Thermoflexus in sediments of some geothermal systems, they appear to be highly focused on chemoorganotrophy, particularly protein degradation, and may interact extensively with other microorganisms in situ.

Sign in / Sign up

Export Citation Format

Share Document