scholarly journals A New Assay Based on Solid-Phase Extraction Procedure with LC-MS to Measure Plasmatic Concentrations of Tenofovir and Emtricitabine in HIV Infected Patients

2008 ◽  
Vol 46 (6) ◽  
pp. 524-528 ◽  
Author(s):  
A. D'Avolio ◽  
M. Sciandra ◽  
M. Siccardi ◽  
L. Baietto ◽  
D. G. de Requena ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Phase Extraction ◽  
Solid Phase Extraction Procedure

scholarly journals Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection

1997 ◽  
Vol 43 (2) ◽  
pp. 312-325 ◽  
Author(s):  
Chi-Kong Lai ◽  
Ting Lee ◽  
Kam-Ming Au ◽  
Albert Yan-Wo Chan
Keyword(s):  
Solid Phase Extraction ◽  
Drug Screening ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Phase Extraction ◽  
Urine Metabolites ◽  
Ultraviolet Spectra ◽  
Solid Phase Extraction Procedure ◽  
Array Detection ◽  
Serum And Urine

Abstract In this HPLC–diode-array detection method for toxicological drug screening, a mixed-mode solid-phase extraction procedure is optimized for isolation of a broad range of drugs from serum and urine. Basic, neutral, and weakly acidic drugs are uniformly recovered. The extract from the solid-phase cartridge is readily injected to a reversed-phase HPLC column for separation by gradient elution. Unknown drugs and metabolites in urine and serum samples from acute drug poisoning cases are rapidly identified by matching their retention times and ultraviolet spectra with hundreds of reference compounds in the library. Urine metabolites of common toxicants from various medications and drugs of abuse are recorded, with their changes of retention times and ultraviolet spectra as related to their metabolic transformations. Glucuronide conjugates of common benzodiazepines, tricyclic antidepressants, and beta-blockers are examined directly without chemical or enzymatic hydrolysis. The system is reliable for diverse clinical investigations of drug overdoses, drug-induced psychoses, and substance abuse.

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