A Bioanalytical Method for Eliglustat Quantification in Rat Plasma

2019 ◽  
Vol 57 (7) ◽  
pp. 600-605 ◽  
Author(s):  
Venkata Krishna Reddy ◽  
Narayana Swamy ◽  
Rajeswari Rathod ◽  
Pinaki Sengupta
Keyword(s):  
Extraction Method ◽  
Ammonium Acetate ◽  
Rat Plasma ◽  
Liquid Extraction ◽  
Good Recovery ◽  
Sprague Dawley ◽  
Bioanalytical Method ◽  
Liquid Liquid Extraction ◽  
Dose Oral ◽  
First Time

Abstract A simple and sensitive bioanalytical HPLC–UV method has been developed and validated for quantification of eliglustat in rat plasma. The liquid–liquid extraction method was found to be more efficient compared to protein precipitation technique. Chromatographic separation of eliglustat was achieved using Kromasil C18 column with a mobile phase consisting of a mixture of methanol and ammonium acetate (pH 3.2) in a ratio of 60:40. Detection wavelength was set at 282 nm. The developed method was specific, accurate, precise with good recovery and stability profile. The calibration curve constructed over a range of 0.3–10 μg/mL was linear (R2 > 0.997). Accuracy in intra and inter-day assay were found to be 96.27–107.35% and 96.80–106.57%, respectively. The corresponding precision (%CV) values were within 4.31–10.90% and 4.82–9.97%, respectively. Till date, no method is available for bioanalysis of eliglustat in any type of biological matrix. This is the first time to report a bioanalytical method for this molecule. The developed bioanalytical method was applied to quantitate eliglustat in the plasma samples of a single dose oral pharmacokinetic study in Sprague Dawley rat.

scholarly journals Homogeneous Liquid-Liquid Extraction Method for Selective Separation and Preconcentration of Trace Amounts of Palladium

2009 ◽  
Vol 6 (4) ◽  
pp. 1077-1084 ◽  
Author(s):  
Mohammad Reza Jamali ◽  
Yaghoub Assadi ◽  
Reyhaneh Rahnama Kozani ◽  
Farzaneh Shemirani
Keyword(s):  
Extraction Method ◽  
Limit Of Detection ◽  
Liquid Extraction ◽  
Selective Separation ◽  
Complexing Agent ◽  
Homogeneous Liquid ◽  
Liquid Liquid Extraction ◽  
Relative Standard ◽  
Separation And Preconcentration ◽  
Ternary Component System

A simple and effective homogeneous liquid-liquid extraction method for selective separation, preconcentration and spectrophotometric determination of palladium(II) ion was developed by using a ternary component system (water / tetrabutylammonium ion (TBA+) / chloroform). The phase separation phenomenon occurred by an ion–pair formation of TBA+and perchlorate ion. Thio-Michler’s ketone (TMK), 4, 4ˊ-bis (dimethylamino) thiobenzophenone, was used as a complexing agent. After optimization of complexation and extraction conditions ([TMK]=5.0x10-2mol L-1, [TBA+] = 2.0×10-2mol L-1, [CHCl3] = 60.0 µL, [ClO4-] = 2.5×10-2mol L-1and pH= 3.0), a preconcentration factor 10 was obtained for 10 mL of sample. The analytical curve was linear in the range of 2-100 ng mL-1and the limit of detection was 0.4 ng mL-1. The relative standard deviation was 3.2% (n=10). Accuracy and application of the method was estimated by using test samples of natural and synthetic water spiked with different amounts of palladium(II) ion. The method is very simple and inexpensive.

Salting-out assisted liquid-liquid extraction method combined with GC-MS for the determination of topiramate in aqueous solutions: development and application of the methodology

2019 ◽  
Vol 55 (13) ◽  
pp. 2303-2312 ◽  
Author(s):  
Janiele Mayara Ferreira De Almeida ◽  
Elania Maria Fernandes Silva ◽  
Lourena Mafra Veríssimo ◽  
Nedja Suely Fernandes
Keyword(s):  
Aqueous Solutions ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Salting Out ◽  
Liquid Liquid Extraction

Tuning the structure and solubility of nanojars by peripheral ligand substitution, leading to unprecedented liquid–liquid extraction of the carbonate ion from water into aliphatic solvents

2016 ◽  
Vol 45 (20) ◽  
pp. 8327-8339 ◽  
Author(s):  
Basil M. Ahmed ◽  
Brice Calco ◽  
Gellert Mezei
Keyword(s):  
Solvent Extraction ◽  
Ligand Substitution ◽  
Liquid Extraction ◽  
Hydration Energy ◽  
Carbonate Ion ◽  
Liquid Liquid Extraction ◽  
First Time

Carbonate, an anion with large hydration energy, is extracted from water by solvent extraction for the first time, using functionalized nanojars.

Optimization of a liquid–liquid extraction method for HPLC–DAD determination of penicillin-V in human plasma

2002 ◽  
Vol 72 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Andrei Medvedovici ◽  
Mihaela Ionescu ◽  
Constantin Mircioiu ◽  
Victor David
Keyword(s):  
Human Plasma ◽  
Extraction Method ◽  
Liquid Extraction ◽  
Penicillin V ◽  
Liquid Liquid Extraction

Determination of Parabens from Water Samples Using Cloud Point Extraction, Vortex Extraction and Liquid–Liquid Extraction Method Coupled with High Performance Liquid Chromatography

2020 ◽  
Vol 17 (2) ◽  
pp. 765-772
Author(s):  
Noorashikin Md Saleh ◽  
N. M. Hafiz ◽  
Nik Nur Atiqah NikWee
Keyword(s):  
Cloud Point ◽  
Water Samples ◽  
Extraction Method ◽  
Optimum Parameter ◽  
Cloud Point Extraction ◽  
Liquid Extraction ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Liquid Liquid Extraction ◽  
Method Of Extraction

A straightforward and efficient way for extraction of parabens that is methylparaben, ethylparaben, propylparaben and benzylparaben in environmental water samples was developed through optimizing parameters for each method of extraction. In this study, methods involved were cloud point extraction, vortex extraction, and liquid–liquid extraction. The parameters affecting the method of extraction were such as salt concentration, surfactant concentration, type of solvent, temperature, ratio of solvent to water and extraction time. The optimum parameter for cloud point extraction method were 1.0 M of salt, 1.0% v/v of surfactant, ratio of surfactant to water is 1:1, extraction time is 1 minute at 30 °C while vortex extraction method, optimum parameter is 1.0 M salt, using acetonitrile as a solvent, ratio 1 solvent: 4 water, and extracted at 1 minute. For the liquid–liquid extraction method, the optimum parameter was at 1.0 M salt, acetonitrile as a solvent, ratio of solvent to water is 1:1 and extraction time at 1 minute. The correlation coefficient for the calibration of paraben at concentration 0.2 ppm–1.0 ppm was in the range from 0.9703 to 0.9942. The limit of detection of studied paraben were 0.1627, 0.0837, 0.1156 and 0.1918 ppm, respectively. Percentage recovery for cloud point extraction, vortex extraction and liquid–liquid extraction were between 41%–147.9%, 26.5%–134.7%, and 31.4%–142.4% respectively. Each sample is repeated with triplication which the value of the relative standard deviation is less than 17.9%. Thus, the most suitable, efficient and effective method in extraction of paraben from water samples is cloud point extraction. The cloud point extraction shows the potential to be explore on the future extraction of others organic pollutants from water samples.

Pharmacokinetic Analysis of Glaucocalyxin A in Rat Plasma by Liquid-Liquid Extraction and HPLC

Planta Medica ◽  
2009 ◽  
Vol 75 (06) ◽  
pp. 629-631 ◽  
Author(s):  
Luye Cao ◽  
Jin Sun ◽  
Yun Shen Li ◽  
Zijun Chen ◽  
Yikui Li
Keyword(s):  
Rat Plasma ◽  
Liquid Extraction ◽  
Pharmacokinetic Analysis ◽  
Liquid Liquid Extraction
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