Emergence and Recovery of Ceftazidime-avibactam Resistance in blaKPC-33-Harboring Klebsiella pneumoniae Sequence Type 11 Isolates in China

2020 ◽  
Vol 71 (Supplement_4) ◽  
pp. S436-S439
Author(s):  
Qingyu Shi ◽  
Dandan Yin ◽  
Renru Han ◽  
Yan Guo ◽  
Yonggui Zheng ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Polymyxin B ◽  
Sequence Type ◽  
First Report ◽  
Clinical Strains

Abstract This is the first report of ceftazidime–avibactam resistance caused by the blaKPC-33 mutation through the D179Y variant during the treatment of blaKPC-2-positive Klebsiella pneumoniae-related infections in China. The blaKPC-33-containing K. pneumoniae was susceptible to meropenem–vaborbactam, cefepime–zidebactam, tigecycline, and polymyxin B. The blaKPC-33 gene was located on a 77 551-bp transformable plasmid harboring qnrS1 and blaLAP-2. Detecting blaKPC-33-positive K. pneumoniae clinical strains is important for infection control.

First report of polymyxin B activity in Klebsiella pneumoniae biofilm

2019 ◽  
Vol 31 (3) ◽  
pp. 127-131 ◽  
Author(s):  
Karina Marjorie Silva Herrera ◽  
Fernanda Kelen da Silva ◽  
Michael Eder de Oliveira ◽  
Magna Cristina de Paiva ◽  
Adriana Cristina Soares ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Polymyxin B ◽  
First Report

scholarly journals Complete Nucleotide Sequences of Two blaKPC-2-Bearing IncN Plasmids Isolated from Sequence Type 442 Klebsiella pneumoniae Clinical Strains Four Years Apart

2014 ◽  
Vol 58 (5) ◽  
pp. 2958-2960 ◽  
Author(s):  
P. J. Perez-Chaparro ◽  
L. T. Cerdeira ◽  
M. G. Queiroz ◽  
C. P. S. de Lima ◽  
C. E. Levy ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nucleotide Sequences ◽  
Sequence Type ◽  
Clinical Strains

scholarly journals First Report of Chronic Pulmonary Infection by KPC-3-Producing and Colistin-Resistant Klebsiella pneumoniae Sequence Type 258 (ST258) in an Adult Patient with Cystic Fibrosis: TABLE 1

2015 ◽  
Vol 53 (4) ◽  
pp. 1442-1444 ◽  
Author(s):  
Emanuele Delfino ◽  
Daniele Roberto Giacobbe ◽  
Valerio Del Bono ◽  
Erika Coppo ◽  
Anna Marchese ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Klebsiella Pneumoniae ◽  
Adult Patient ◽  
Pulmonary Infection ◽  
Lung Infection ◽  
Sequence Type ◽  
Content Type ◽  
First Report ◽  
Chronic Lung Infection

The spread ofKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniaecontinues to increase, and the possible development of KPC-producingK. pneumoniaeinfections in cystic fibrosis (CF) patients is a matter of concern. Here, we describe the establishment of a chronic lung infection due to a colistin-resistant KPC-producingK. pneumoniaeisolate in an Italian CF patient.

scholarly journals Outbreak of NDM-1-Producing Klebsiella pneumoniae in a Neonatal Unit in Colombia

2013 ◽  
Vol 57 (4) ◽  
pp. 1957-1960 ◽  
Author(s):  
Javier Antonio Escobar Pérez ◽  
Narda María Olarte Escobar ◽  
Betsy Castro-Cardozo ◽  
Ismael Alberto Valderrama Márquez ◽  
Martha Isabel Garzón Aguilar ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
South America ◽  
Molecular Analysis ◽  
Multilocus Sequence Typing ◽  
Sequence Type ◽  
Neonatal Unit ◽  
Content Type ◽  
First Report ◽  
Sequence Types

ABSTRACTSix multiresistant, NDM-1-producingKlebsiella pneumoniaestrains were recovered from an outbreak that affected six neonatal patients in a Colombian hospital. Molecular analysis showed that all of the isolates harbored theblaNDM-1,qnrA, andintI1genes and were clonally related. Multilocus sequence typing showed that the isolates belonged to a new sequence type (ST1043) that was different from the sequence types that had previously been reported. This is the first report of NDM-1-producing isolates in South America.

scholarly journals Emerging carbapenem-resistant Klebsiella pneumoniae sequence type 16 causing multiple outbreaks in a tertiary hospital in southern Vietnam

2021 ◽  
Author(s):  
To Nguyen Thi Nguyen ◽  
Phuong Luong Nha Nguyen ◽  
Ngan Thi Quynh Le ◽  
Lan Phu Huong Nguyen ◽  
Thuy Bich Duong ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Type Species ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Health Concern ◽  
Environmental Isolates ◽  
Content Type ◽  
Link Type ◽  
Carbapenem Resistant

The emergence of carbapenem resistance in Klebsiella pneumoniae represents a major global public health concern. Nosocomial outbreaks caused by multidrug-resistant K. pneumoniae are commonly reported to result in high morbidity and mortality due to limited treatment options. Between October 2019 and January 2020, two concurrent high-mortality nosocomial outbreaks occurred in a referral hospital in Ho Chi Minh City, Vietnam. We performed genome sequencing and phylogenetic analysis of eight K. pneumoniae isolates from infected patients and two environmental isolates for outbreak investigation. We identified two outbreaks caused by two distinct lineages of the international sequence type (ST) 16 clone, which displayed extensive drug resistance, including resistance to carbapenem and colistin. Carbapenem-resistant ST16 outbreak strains clustered tightly with previously described ST16 K. pneumoniae from other hospitals in Vietnam, suggesting local persistence and transmission of this particular clone in this setting. We found environmental isolates from a hospital bed and blood pressure cuff that were genetically linked to an outbreak case cluster, confirming the potential of high-touch surfaces as sources for nosocomial spread of K. pneumoniae . Further, we found colistin resistance caused by disruption of the mgrB gene by an ISL3-like element, and carbapenem resistance mediated by a transferable IncF/bla OXA-181 plasmid carrying the ISL3-like element. Our study highlights the importance of coordinated efforts between clinical and molecular microbiologists and infection control teams to rapidly identify, investigate and contain nosocomial outbreaks. Routine surveillance with advanced sequencing technology should be implemented to strengthen hospital infection control and prevention measures.

scholarly journals Klebsiella pneumoniae Sequence Type 11 from Companion Animals Bearing ArmA Methyltransferase, DHA-1 β-Lactamase, and QnrB4

2013 ◽  
Vol 57 (9) ◽  
pp. 4532-4534 ◽  
Author(s):  
Laura Hidalgo ◽  
Belen Gutierrez ◽  
Cristina M. Ovejero ◽  
Laura Carrilero ◽  
Stephanie Matrat ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multilocus Sequence Typing ◽  
Companion Animals ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Resistance Determinant ◽  
Epidemic Clone ◽  
Content Type ◽  
First Report

ABSTRACTSevenKlebsiella pneumoniaeisolates from dogs and cats in Spain were found to be highly resistant to aminoglycosides, and ArmA methyltransferase was responsible for this phenotype. All isolates were typed by multilocus sequence typing (MLST) as ST11, a human epidemic clone reported worldwide and associated with, among others, OXA-48 and NDM carbapenemases. In the seven strains,armAwas borne by an IncR plasmid, pB1025, of 50 kb. The isolates were found to coproduce DHA-1 and SHV-11 β-lactamases, as well as the QnrB4 resistance determinant. This first report of the ArmA methyltransferase in pets illustrates their importance as a reservoir for human multidrug-resistantK. pneumoniae.

scholarly journals Comparison of Commensal and Clinical Isolates for Diversity of Plasmids in Escherichia coli and Klebsiella pneumoniae

2020 ◽  
Vol 64 (5) ◽  
Author(s):  
Judith Rodríguez-Navarro ◽  
Elisenda Miró ◽  
Maryury Brown-Jaque ◽  
Juan Carlos Hurtado ◽  
Albert Moreno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Plasmid Content ◽  
Clinical Strains ◽  
C And N

ABSTRACT In this study, the plasmid content of clinical and commensal strains was analyzed and compared. The replicon profile was similar in both populations, except for L, M, A/C, and N (detected only in clinical strains) and HI1 (only in commensal strains). Although I1 and F were the most frequent replicons, only IncI1, sequence type 12 (ST12) was associated with blaCMY-2 in both populations. In contrast, the widespread resistant IncF plasmids were not linked to a single epidemic plasmid.

The emergence of multidrug-resistantKlebsiella pneumoniaeof international clones ST13, ST16, ST35, ST48 and ST101 in a teaching hospital in the Paris region

2012 ◽  
Vol 141 (8) ◽  
pp. 1705-1712 ◽  
Author(s):  
G. MARCADE ◽  
S. BRISSE ◽  
S. BIALEK ◽  
E. MARCON ◽  
V. LEFLON-GUIBOUT ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Teaching Hospital ◽  
Sequence Type ◽  
Control Measures ◽  
Incidence Density ◽  
Spatial Relationships ◽  
Cross Contamination ◽  
Infection Control Measures ◽  
Paris Region

SUMMARYDespite infection control measures, an important increase in the extended-spectrum β-lactamase (ESBL)-producingKlebsiella pneumoniaeincidence density occurred in our hospital from 2006 onwards. This study, focusing on the 2005–2007 period, was performed in an attempt to explain this increase. ESBLs were characterized, isolates were typed by ERIC2-PCR, and sequence type (ST) of clustered isolates was determined. Temporal-spatial relationships of patients were analysed to assess possible cross-contamination. Of the 74 ESBL-producing isolates, 30 (40%) were detected at admission, 53 (71·5%) produced CTX-M enzymes, 40 displayed unique ERIC2-PCR profiles and 34 were assigned into six clusters: ST16 (n = 21), ST101, ST48, ST35, ST13, and ST436. Relationships were identified in 22 of the 34 patients harbouring clustered isolates. This study highlights the complex epidemiology of ESBL-producingK. pneumoniaein the mid-2000s with potential cross-contamination for only 30% of the 74 patients in our hospital, and the emergence of clones that are currently spreading worldwide.

scholarly journals Clonal Dissemination of OXA-232 Carbapenemase-Producing Klebsiella pneumoniae in Neonates

2017 ◽  
Vol 61 (8) ◽  
Author(s):  
Dandan Yin ◽  
Dong Dong ◽  
Ke Li ◽  
Lei Zhang ◽  
Jianliang Liang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Content Type ◽  
First Report ◽  
Clone Sequence

ABSTRACT Five OXA-232 carbapenemase-producing Klebsiella pneumoniae isolates, belonging to the pandemic clone sequence type 15 (ST15), were isolated from neonates and coproduced bla CTX-M-15 and bla SHV-1 genes. All isolates were resistant to ertapenem (MICs of >32 μg/ml) and meropenem (MICs of 4 to 8 μg/ml) and susceptible or intermediate to imipenem (MICs of 1 to 2 μg/ml). The bla OXA-232 gene was located on a ColE-type transformable plasmid of 6,141 bp. To the best of our knowledge, this is the first report of OXA-232 carbapenemase among clinical isolates in China.

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