Use of the Berthelot Reaction in the Automated Analysis of Serum Urea Nitrogen

1966 ◽  
Vol 12 (3) ◽  
pp. 151-157 ◽  
Author(s):  
Alan A Wilcox ◽  
Wallace E Carroll ◽  
Rex E Sterling ◽  
H A Davis ◽  
Arnold G Ware
Keyword(s):  
Automated Analysis ◽  
Serum Urea ◽  
Urea Nitrogen ◽  
Serum Urea Nitrogen ◽  
Enzymatic Action ◽  
Diacetyl Monoxime ◽  
Automated Determination

Abstract A method is presented for the automated determination of urea nitrogen using the Berthelot reaction for measuring the ammonia released by the enzymatic action of urease. The method compares favorably with the automated diacetyl monoxime method and employs less dangerous and less expensive reagents.

Combination of Chlorogenic Acid and Bamboo Charcoal Particles Improve Husbandry Performance and Environment

2011 ◽  
Vol 356-360 ◽  
pp. 142-145
Author(s):  
Jian Hui Wang ◽  
Dong Min Liu ◽  
Yong Le Liu ◽  
Chi Lin Li ◽  
Fa Xiang Wang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Chlorogenic Acid ◽  
Ammonia Concentration ◽  
Bamboo Charcoal ◽  
Serum Urea ◽  
Growth Promoters ◽  
Urea Nitrogen ◽  
Serum Urea Nitrogen ◽  
Antibiotic Growth Promoters ◽  
Ad Libitum

The objective of this study was to determine the effects of chlorogenic acid (CA) and bamboo charcoal particles activated by microwave (BCP) on performance, serum urea nitrogen and husbandry environment of broilers. The diets were devoid of antibiotic growth promoters (AGP). Two hundred and forty Avian broilers were allocated to a 2 × 2 factorial experiment involving two levels of CA (0 or 1000 mg CA/kg feed), and two levels of BCP supplementation (0 or 100 mg/kg feed). The diets and water were offered ad libitum. The growth performance and serum urea nitrogen was significantly increased when CA supplemented (P<0.05), while BCP significantly affects the ammonia concentration of the husbandry environment (P<0.05). These results indicate that CA and BCP combination can effectively improve the performance and husbandry environment.

Rapid-flow enthalpimetric determination of urea in serum, with use of an immobilized urease reactor.

1976 ◽  
Vol 22 (8) ◽  
pp. 1314-1318 ◽  
Author(s):  
L D Bowers ◽  
L M Canning ◽  
C N Sayers ◽  
P W Carr
Keyword(s):  
Carbon Dioxide ◽  
Measured Temperature ◽  
Serum Urea ◽  
Serum Samples ◽  
Thermal Detector ◽  
Temperature Changes ◽  
Rapid Flow ◽  
Diacetyl Monoxime ◽  
Flow Stream

Abstract We used a differential thermal detector in conjunction with an immobilized urease reactor to determine urea in serum. Samples (120 mul) are introduced into a flow stream and passed through an "adiabatic" column, which is packed with enough insolubilized urease to completely convert urea to ammonia and carbon dioxide. Measured temperature changes are directly proportional to the serum urea concentration. Urea in the presence of protein, bilirubin, and hemoglobin can thus be rapidly, simply, and inexpensively measured. Results correlate well with those obtained by the manual diacetyl monoxime and urease/indophenol methods.

Sign in / Sign up

Export Citation Format

Share Document