Column-Chromatographic Analysis of Naturally Fluorescing Compounds: II. Rapid Analysis of Indoleacetic Acid and 5-Hydroxyindoleacetic Acid in Biological Samples

1972 ◽  
Vol 18 (11) ◽  
pp. 1376-1378 ◽  
Author(s):  
D D Chilcote
Keyword(s):  
Acetic Acid ◽  
Ammonium Acetate ◽  
Indoleacetic Acid ◽  
Exchange Resin ◽  
Biological Extracts ◽  
Strong Anion Exchange ◽  
Hydroxyindoleacetic Acid ◽  
Acetic Acid Buffer ◽  
Indole 3 Acetic Acid

Abstract A previously described [Clin. Chem. 18, 778( 1972)] chromatographic separation and fluorometric system has been adapted to determination of 5-hydroxyindole-3-acetic acid and indole-3-acetic acid in body fluids and biological extracts. A strong anion-exchange resin, "Aminex A-27," is used. The indoleacetic acids are eluted at 60°C with a single ammonium acetate-acetic acid buffer. No gradient programming or post-column reaction designed to enhance the fluorescence of the desired compounds is necessary. The sensitivity of the detector allows analysis of 100 ng of each acid in the sample loaded onto the column.

Column-Chromatographic Analysis of Naturally Fluorescing Compounds: III. Rapid Analysis of Serotonin and Tryptamine

1974 ◽  
Vol 20 (4) ◽  
pp. 421-423 ◽  
Author(s):  
D D Chilcote
Keyword(s):  
Ammonium Acetate ◽  
Exchange Resin ◽  
Detection System ◽  
Cation Exchange Resin ◽  
Rapid Analysis ◽  
Buffer Solution ◽  
Column Temperature ◽  
Strong Cation Exchange ◽  
Acetic Acid Buffer

Abstract A previously described [Clin. Chem. 18, 778 (1972)] chromatographic separation and fluorimetric detection system has been adapted to the determination of serotonin and tryptamine. A strong cation-exchange resin, "Aminex A-6," is used. At a column temperature of 60 °C, serotonin and tryptamine are eluted in 0.5 and 1.0 h, respectively, with an ammonium acetate-acetic acid buffer solution (6 mol of acetate per liter). The sensitivity of the detector allows reliable quantitation of 100 ng of each amine in the sample loaded onto the column.

Determination of indole-3-acetic acid in plant tissues by capillary electrophoresis

1997 ◽  
Vol 779 (1-2) ◽  
pp. 342-346 ◽  
Author(s):  
M. Brüns ◽  
A. Bazzanella ◽  
H. Lochmann ◽  
K. Bächmann ◽  
C. Ullrich-Eberius
Keyword(s):  
Acetic Acid ◽  
Capillary Electrophoresis ◽  
Plant Tissues ◽  
Indole 3 Acetic Acid

Determination of Plant Hormone Indole-3-Acetic Acid in Aqueous Solution

2012 ◽  
Vol 25 (1) ◽  
pp. 303-307 ◽  
Author(s):  
Jana Bulíčková ◽  
Romana Sokolová ◽  
Stefania Giannarelli ◽  
Beatrice Muscatello
Keyword(s):  
Aqueous Solution ◽  
Acetic Acid ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid

Analytical Methods for Diethylstilbestrol in Feeds and Premixes

1966 ◽  
Vol 49 (5) ◽  
pp. 895-898
Author(s):  
Loyal R Stone
Keyword(s):  
Acetic Acid ◽  
Analytical Methods ◽  
Sodium Acetate ◽  
Buffer System ◽  
Official Method ◽  
Sodium Acetate Solution ◽  
Acetate Solution ◽  
Acid Buffer System ◽  
Acetic Acid Buffer

Abstract Methods are presented in which diethylstilbestrol is extracted from feeds in the Goldfisch apparatus, transferred into alkaline sodium acetate solution to avoid emulsions, and measured colorimetrically in a sodium acetate-acetic acid buffer system. The procedure is rapid, and results agree closely with those obtained by the official method. Procedures are also presented for determination of diethylstilbestrol in molasses and fat mixtures.

Gold nanoparticle-doped three-dimensional reduced graphene hydrogel modified electrodes for amperometric determination of indole-3-acetic acid and salicylic acid

Nanoscale ◽  
2019 ◽  
Vol 11 (21) ◽  
pp. 10247-10256 ◽  
Author(s):  
Xiaodong Cao ◽  
Xueting Zhu ◽  
Shudong He ◽  
Xuan Xu ◽  
Yongkang Ye ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Gold Nanoparticle ◽  
Three Dimensional ◽  
Modified Electrodes ◽  
Amperometric Determination ◽  
Reduced Graphene ◽  
Indole 3 Acetic Acid

Three-dimensional (3D) networked nanomaterials have attracted great interest because of their unique porous and 3D-networked structures.

scholarly journals Determination of Indole-3-Acetic Acid in Soil Using Excitation-Emission Matrix Fluorescence with Trilinear Decomposition-based Calibration Methods

2009 ◽  
Vol 25 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Yuan-Na LI ◽  
Hai-Long WU ◽  
Shao-Hua ZHU ◽  
Jin-Fang NIE ◽  
Yong-Jie YU ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Trilinear Decomposition ◽  
Excitation Emission Matrix ◽  
Calibration Methods ◽  
Indole 3 Acetic Acid
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