Column-Chromatographic Analysis of Naturally Fluorescing Compounds: III. Rapid Analysis of Serotonin and Tryptamine

1974 ◽  
Vol 20 (4) ◽  
pp. 421-423 ◽  
Author(s):  
D D Chilcote
Keyword(s):  
Ammonium Acetate ◽  
Exchange Resin ◽  
Detection System ◽  
Cation Exchange Resin ◽  
Rapid Analysis ◽  
Buffer Solution ◽  
Column Temperature ◽  
Strong Cation Exchange ◽  
Acetic Acid Buffer

Abstract A previously described [Clin. Chem. 18, 778 (1972)] chromatographic separation and fluorimetric detection system has been adapted to the determination of serotonin and tryptamine. A strong cation-exchange resin, "Aminex A-6," is used. At a column temperature of 60 °C, serotonin and tryptamine are eluted in 0.5 and 1.0 h, respectively, with an ammonium acetate-acetic acid buffer solution (6 mol of acetate per liter). The sensitivity of the detector allows reliable quantitation of 100 ng of each amine in the sample loaded onto the column.

Ion Exchange Method for Rapid Determination of Alkalinity of Water-Soluble Tea Ash Containing High Levels of Manganese

1980 ◽  
Vol 63 (3) ◽  
pp. 460-461
Author(s):  
Saidul Z Qureshi ◽  
Fadhil M Najib ◽  
Fahmi A Mohammed
Keyword(s):  
Ion Exchange ◽  
Cation Exchange ◽  
Exchange Resin ◽  
Rapid Determination ◽  
Cation Exchange Resin ◽  
Water Soluble ◽  
Exchange Method ◽  
Strong Cation Exchange ◽  
Ion Exchange Method

Abstract An ion exchange method to determine the alkalinity of water-soluble tea ash containing high levels of manganese is described. A chromatographic column containing a strong cation exchange resin (20–50 mesh) in Na+ form, with a bed volume of 5 mL is used. The present ion exchange method is compared to pH titrations and also to the official AOAC methods (31.012, 31.015, 31.016). Results with the new method are accurate and precise.

Determination of formaldehyde in fruit juice based on magnetic strong cation-exchange resin modified with 2,4-dinitrophenylhydrazine

2012 ◽  
Vol 131 (1) ◽  
pp. 380-385 ◽  
Author(s):  
Hui Wang ◽  
Jie Ding ◽  
Xiaobo Du ◽  
Xin Sun ◽  
Ligang Chen ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Fruit Juice ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Strong Cation Exchange

Column-Chromatographic Analysis of Naturally Fluorescing Compounds: II. Rapid Analysis of Indoleacetic Acid and 5-Hydroxyindoleacetic Acid in Biological Samples

1972 ◽  
Vol 18 (11) ◽  
pp. 1376-1378 ◽  
Author(s):  
D D Chilcote
Keyword(s):  
Acetic Acid ◽  
Ammonium Acetate ◽  
Indoleacetic Acid ◽  
Exchange Resin ◽  
Biological Extracts ◽  
Strong Anion Exchange ◽  
Hydroxyindoleacetic Acid ◽  
Acetic Acid Buffer ◽  
Indole 3 Acetic Acid

Abstract A previously described [Clin. Chem. 18, 778( 1972)] chromatographic separation and fluorometric system has been adapted to determination of 5-hydroxyindole-3-acetic acid and indole-3-acetic acid in body fluids and biological extracts. A strong anion-exchange resin, "Aminex A-27," is used. The indoleacetic acids are eluted at 60°C with a single ammonium acetate-acetic acid buffer. No gradient programming or post-column reaction designed to enhance the fluorescence of the desired compounds is necessary. The sensitivity of the detector allows analysis of 100 ng of each acid in the sample loaded onto the column.

Polarographic Determination of Zinc in Compounded Rubber

1950 ◽  
Vol 23 (4) ◽  
pp. 975-980
Author(s):  
F. C. J. Poulton ◽  
L. Tarrant
Keyword(s):  
Zinc Oxide ◽  
Ammonium Acetate ◽  
Potassium Thiocyanate ◽  
Acetate Buffer Solution ◽  
Acid Electrolyte ◽  
Buffer Solution ◽  
Ammonium Acetate Buffer ◽  
Ammonium Acetate Buffer Solution ◽  
Gravimetric Procedure

Abstract Reasons are advanced for the unsatisfactory nature of some of the older methods for the determination of very small amounts of zinc in compounded rubber, particularly in latex mixings. The polarographic technique offers a possible solution, but most of the commoner electrolytes for the electroreduction of this metal are alkaline, and give rise to similar errors as are met in the gravimetric procedure. The development of a suitable acid electrolyte was therefore undertaken, and ways of dealing with likely interferences were examined. The electroltye finally recommended is a potassium thiocyanate-ammonium acetate buffer solution; iron, when present, is reduced to the ferrous condition by potassium iodide. The method was used to determine zinc oxide in a series of mixings of known composition ranging from 0.8 to 40 per cent. In all except the highest proportions of zinc oxide, the figures obtained agree well with the theoretical.

Determination of Potential Genotoxic Impurity, 5-Amino-2-Chloropyridine, in Active Pharmaceutical Ingredient Using the HPLC-UV System

2020 ◽  
Author(s):  
Murat Soyseven ◽  
Rüstem Keçili ◽  
Hassan Y Aboul-Enein ◽  
Göksel Arli
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Orthophosphoric Acid ◽  
Limit Of Detection ◽  
Detection System ◽  
Active Pharmaceutical Ingredient ◽  
Limit Of Quantification ◽  
Column Temperature ◽  
Water Ph

Abstract A novel analytical method, based on high-performance liquid chromatography with a UV (HPLC-UV) detection system for the sensitive detection of a genotoxic impurity (GTI) 5-amino-2-chloropyridine (5A2Cl) in a model active pharmaceutical ingredient (API) tenoxicam (TNX), has been developed and validated. The HPLC-UV method was used for the determination of GTI 5A2Cl in API TNX. The compounds were separated using a mobile phase composed of water (pH 3 adjusted with orthophosphoric acid): MeOH, (50:50: v/v) on a C18 column (150 × 4.6 mm i.d., 2.7 μm) at a flow rate of 0.7 mL min−1. Detection was carried out in the 254 nm wavelength. Column temperature was maintained at 40°C during the analyses and 10 μL volume was injected into the HPLC-UV system. The method was validated in the range of 1–40 μg mL−1. The obtained calibration curves for the GTI compound was found linear with equation, y = 40766x − 1125,6 (R2 = 0.999). The developed analytical method toward the target compounds was accurate, and the achieved limit of detection and limit of quantification values for the target compound 5A2Cl were 0.015 and 0.048 μg mL−1, respectively. The recovery values were calculated and found to be between 98.80 and 100.03%. The developed RP-HPLC-UV analytical method in this research is accurate, precise, rapid, simple and appropriate for the sensitive analysis of target GTI 5A2Cl in model API TNX.

An improved separation scheme for Sr through fluoride coprecipitation combined with a cation-exchange resin from geological samples with high Rb/Sr ratios for high-precision determination of Sr isotope ratios

2020 ◽  
Vol 35 (5) ◽  
pp. 953-960
Author(s):  
Wen-Gang Liu ◽  
Shuang Wei ◽  
Jian Zhang ◽  
Cong Ao ◽  
Fu-Tian Liu ◽  
...  
Keyword(s):  
Cation Exchange ◽  
High Precision ◽  
Exchange Resin ◽  
Cation Exchange Resin ◽  
Separation Scheme ◽  
Geological Samples ◽  
Sr Isotope ◽  
Precision Determination ◽  
High Precision Determination

A new separation method for Sr involving HF coprecipitation combined with AG50 resin from samples with high Rb/Sr ratios.

Sign in / Sign up

Export Citation Format

Share Document