Isolation of 2-Acetamido-1-β-(L-β-Aspartamido)-1,2-Dideoxy-D-Glucose from Normal Human Urine
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Abstract A glycopeptide was isolated from normal human urine by fractionation on a column of Sephadex G-10 and preparative ion-exchange chromatography. Elution behavior during ion-exchange chromatography in two different solvent systems, amino acids formed upon hydrolysis, and migration on high-voltage electrophoresis and thin-layer chromatography were essentially identical for this substance and for authentic 2-acetamido-l-β-(L-β-aspartamido)-1,2-dideoxy-D-glucose. A technique was developed to permit analytical-scale fractionation of individual urines followed by analysis for this glycopeptide; urine from two normal individuals contained 7 and 11 µmol of 2-acetamido-1-β-(L-β-aspartamido)-1,2-dideoxy-D-glucose per liter.
1988 ◽
Vol 452
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pp. 459-468
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2013 ◽
Vol 28
(9)
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pp. 1402
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2003 ◽
Vol 38
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pp. 115-128
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1967 ◽
Vol 140
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pp. 371-374
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2001 ◽
Vol 16
(4)
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pp. 339-344
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1970 ◽
Vol 29
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pp. 421-427
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